FastQCFastQC Report
Sat 18 Jun 2016
SRR3549869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269143
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550664.338833370234875No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC31160.24552000838361002No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC25810.2033655781893766No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT14310.1127532516036412No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA13010.1025101190330798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG302.1656815E-645.0000041
CTTCGAA207.033565E-445.014
CGGTTAG351.2120654E-745.01
GCGATAC253.891036E-545.09
GCTAACG406.8157533E-945.01
GCTCGAA207.033565E-445.09
CCGTTCA207.033565E-445.027
CGTTTTT355600.044.506471
ACGGGTC1100.042.9545445
CCCTCGT950.042.6315814
GTTTTTT395350.040.2649572
TAGGGAC5200.040.2403835
CGGTAGT1200.039.37500412
CGCGACC1150.039.13043610
TACGGGT1050.038.571434
GTATTCG356.248805E-638.5714261
TATAGCG356.248805E-638.5714261
GCCCGAA356.248805E-638.57142625
GCTACGA1300.038.0769210
GGCACGT601.5643309E-1037.5000048