##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1269143
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.662554968195074	34.0	31.0	34.0	31.0	34.0
2	32.81985323954826	34.0	31.0	34.0	31.0	34.0
3	32.862143194265734	34.0	31.0	34.0	31.0	34.0
4	36.236067960820805	37.0	37.0	37.0	35.0	37.0
5	36.13836108303004	37.0	37.0	37.0	35.0	37.0
6	36.131293321556356	37.0	36.0	37.0	35.0	37.0
7	36.26449659337049	37.0	37.0	37.0	35.0	37.0
8	36.26452023136873	37.0	37.0	37.0	35.0	37.0
9	38.04963506870384	39.0	39.0	39.0	37.0	39.0
10	37.67223472847425	39.0	38.0	39.0	35.0	39.0
11	37.56793836470752	39.0	37.0	39.0	35.0	39.0
12	37.513341680173156	39.0	37.0	39.0	35.0	39.0
13	37.48568207049954	39.0	37.0	39.0	35.0	39.0
14	38.80754729766465	40.0	38.0	41.0	35.0	41.0
15	38.85655517148186	40.0	38.0	41.0	35.0	41.0
16	38.9432963818892	40.0	38.0	41.0	35.0	41.0
17	38.94965263961587	40.0	38.0	41.0	35.0	41.0
18	38.42620886692831	39.0	38.0	41.0	35.0	41.0
19	37.934409282484324	38.0	37.0	41.0	35.0	41.0
20	37.35379937485374	38.0	35.0	41.0	34.0	41.0
21	37.302031370775396	38.0	35.0	41.0	34.0	41.0
22	37.108557506916085	38.0	35.0	41.0	34.0	41.0
23	37.016023411073455	38.0	35.0	40.0	34.0	41.0
24	36.821176179516414	38.0	35.0	40.0	33.0	41.0
25	36.66182061438309	37.0	35.0	40.0	33.0	41.0
26	36.769625645021875	38.0	35.0	40.0	33.0	41.0
27	36.797541333009754	38.0	35.0	40.0	33.0	41.0
28	36.73303165994691	38.0	35.0	40.0	33.0	41.0
29	36.59041258550061	38.0	35.0	40.0	33.0	41.0
30	36.284272142697866	38.0	35.0	40.0	33.0	41.0
31	35.802054614807	38.0	35.0	40.0	31.0	41.0
32	35.05381899439228	38.0	35.0	40.0	23.0	41.0
33	34.090596567920244	38.0	34.0	40.0	17.0	41.0
34	33.29214989957791	38.0	33.0	41.0	12.0	41.0
35	32.77956384741515	38.0	33.0	41.0	10.0	41.0
36	32.54049386081789	38.0	32.0	41.0	8.0	41.0
37	32.3200151598362	38.0	31.0	41.0	8.0	41.0
38	32.1737613491939	37.0	31.0	41.0	8.0	41.0
39	32.082407577396715	37.0	31.0	40.0	8.0	41.0
40	31.9344006152183	37.0	30.0	40.0	7.0	41.0
41	31.79502861379687	37.0	30.0	40.0	7.0	41.0
42	31.660339299826735	37.0	29.0	40.0	7.0	41.0
43	31.557473822886784	37.0	29.0	40.0	7.0	41.0
44	31.451588197705064	36.0	27.0	40.0	7.0	41.0
45	31.431181513824683	36.0	27.0	40.0	7.0	41.0
46	31.405914069572933	36.0	27.0	40.0	7.0	41.0
47	31.42664301816265	36.0	27.0	40.0	7.0	41.0
48	31.390116795349304	36.0	27.0	40.0	7.0	41.0
49	31.28118738392758	36.0	27.0	40.0	7.0	41.0
50	31.202234106006966	35.0	27.0	40.0	7.0	41.0
51	30.680823989101306	35.0	25.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	13.0
11	16.0
12	20.0
13	27.0
14	33.0
15	69.0
16	111.0
17	239.0
18	414.0
19	833.0
20	1437.0
21	2261.0
22	3561.0
23	6046.0
24	11972.0
25	26827.0
26	49859.0
27	64933.0
28	60594.0
29	48109.0
30	37129.0
31	31571.0
32	30190.0
33	35821.0
34	62715.0
35	84371.0
36	90471.0
37	111797.0
38	208149.0
39	299342.0
40	203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.08178432217646	15.826506548119479	18.149412635140404	37.94229649456366
2	50.8168110291748	18.239946168398674	17.936434271000195	13.006808531426325
3	23.279331013132484	17.58848293691097	46.193218573478326	12.938967476478222
4	21.03206652047878	19.127159035664224	45.785620690497446	14.055153753359551
5	18.83869666381172	22.068829123274526	44.890686077140245	14.20178813577351
6	20.102541636364066	24.32688830179105	44.24781131834632	11.322758743498564
7	58.41272417686581	4.786931023533203	32.99368156307052	3.8066632365304773
8	58.956240549725294	4.476721693300124	32.62248619737886	3.944551559595727
9	54.263152379203916	6.302678263993893	34.36106096791299	5.073108388889196
10	29.98992233341712	19.844729868895783	39.49192486583466	10.67342293185244
11	23.32841925614371	18.46182817854253	44.07659341776301	14.13315914755075
12	21.64925465451884	16.24151100388215	47.56122832494053	14.548006016658485
13	18.4487484861832	18.317400009297614	48.11735163019455	15.116499874324644
14	14.655874081959244	20.896935963874835	47.65932601763552	16.787863936530396
15	14.012132596563193	19.251337319750412	51.85979830484035	14.87673177884604
16	15.759847393083366	18.243019108169843	50.32127979274203	15.67585370600476
17	15.95793381833253	17.930682358095186	47.72519723939698	18.386186584175306
18	16.0934583415738	18.99352555228213	48.67182027557178	16.241195830572284
19	17.592186223301866	20.126888774551016	46.60964130913538	15.671283693011743
20	19.529556559032354	19.04655346166665	46.76163363781701	14.662256341483978
21	18.474592697591994	19.13235939527697	46.846415258170275	15.546632648960756
22	16.553453787319476	19.189563351017185	46.179350947844334	18.07763191381901
23	16.628622621721902	19.077834412670597	47.07239452134235	17.221148444265147
24	17.12809352452797	18.02499797107182	48.16683383984311	16.680074664557107
25	16.148850050782297	19.720236411499727	46.58198485119486	17.54892868652311
26	15.499750619118572	21.2440993646894	46.47860800555966	16.77754201063237
27	15.895844676289434	21.216048940111556	47.06609105514509	15.822015328453926
28	15.091916356155297	20.708147151266644	47.72637913930897	16.473557353269097
29	16.23914720405817	20.474524935330376	46.4291257959111	16.857202064700353
30	16.559520873534346	21.291375361168914	45.466192540950864	16.68291122434588
31	18.246722394560738	22.120281166109727	42.66753234269109	16.965464096638442
32	18.78622030771946	22.821935747193184	42.00653511858002	16.385308826507337
33	18.78298978129336	24.202158464412598	40.064437183201576	16.950414571092463
34	18.511546768173485	24.331458314784072	38.63142293658004	18.52557198046241
35	18.19818570484177	25.384767516347644	36.30229217668931	20.114754602121273
36	18.926787603918548	27.66473123989968	35.79809367423529	17.61038748194648
37	20.016972082736146	26.988842076897562	36.04258936936184	16.95159647100445
38	19.63119995146331	26.611264451681173	34.61942428867354	19.13811130818198
39	19.911625403914297	25.602946240100604	36.34996213980615	18.13546621617895
40	19.580378255247833	25.953970513960993	35.79312969460494	18.672521536186228
41	17.334453249161047	26.903745283234436	35.11921036478947	20.64259110281505
42	17.980400947726142	27.12373625351911	34.91095960029721	19.984903198457545
43	20.02776676859897	25.889044812129132	34.702236075840155	19.380952343431748
44	18.83964218374131	26.580219880659627	34.3248160372787	20.255321898320364
45	19.198466997020823	25.706244292408343	34.11845631264562	20.976832397925214
46	18.768255429057245	26.364326163403177	35.200288698751834	19.66712970878774
47	17.891049314379863	25.413211907562822	37.10637808347838	19.58936069457894
48	18.06187324832584	23.96845745514887	37.364977784221324	20.604691512303972
49	17.84109434476651	23.666836597609567	38.257391011099614	20.23467804652431
50	17.683901656472123	23.463077052782864	38.08806415037549	20.764957140369525
51	17.156143949105815	23.519099108611087	36.19040565168779	23.134351290595305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	386.0
1	726.0
2	1066.0
3	54057.0
4	107048.0
5	73955.5
6	40863.0
7	38767.5
8	36672.0
9	35138.5
10	33605.0
11	31649.5
12	29694.0
13	27546.0
14	25398.0
15	23174.0
16	20950.0
17	19349.5
18	17749.0
19	16320.5
20	14892.0
21	13703.5
22	12515.0
23	11486.0
24	10457.0
25	10812.5
26	11721.5
27	12275.0
28	13489.5
29	14704.0
30	16446.5
31	18189.0
32	19924.0
33	21659.0
34	23444.5
35	25230.0
36	26488.0
37	27746.0
38	30818.5
39	33891.0
40	38107.0
41	42323.0
42	46973.0
43	51623.0
44	57670.5
45	63718.0
46	69454.5
47	75191.0
48	83922.5
49	92654.0
50	88630.0
51	84606.0
52	74454.5
53	64303.0
54	57739.0
55	51175.0
56	47276.0
57	43377.0
58	43393.5
59	43410.0
60	41406.5
61	39403.0
62	37340.5
63	35278.0
64	30067.5
65	24857.0
66	19966.5
67	15076.0
68	12888.0
69	10700.0
70	8448.0
71	6196.0
72	5064.0
73	3932.0
74	3138.0
75	1925.0
76	1506.0
77	1096.5
78	687.0
79	532.0
80	377.0
81	239.0
82	101.0
83	104.0
84	107.0
85	60.5
86	14.0
87	14.5
88	15.0
89	11.5
90	8.0
91	6.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1269143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.322393129055705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92549396820013	25.18738450816024
2	9.667672101198024	6.249645965954532
3	3.444361404492952	3.3399001018370287
4	1.7429975688779675	2.253514105770481
5	0.9982704082413761	1.6133244292140345
6	0.6714260484860056	1.3021258017751856
7	0.45678885303941424	1.0335156219437278
8	0.36252962328565386	0.9374260003813896
9	0.2736089915942179	0.7959327650957528
>10	3.5609184336011386	29.465109156517137
>50	0.8032345357988047	16.515194380431833
>100	0.08698209682478215	5.0322091444957815
>500	0.0034792838710146056	0.8307490814262549
>1k	0.001988162212008346	1.0206437660609162
>5k	0.0	0.0
>10k+	2.4852027650104327E-4	4.423325170935811
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55066	4.338833370234875	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	3116	0.24552000838361002	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2581	0.2033655781893766	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	1431	0.1127532516036412	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1301	0.1025101190330798	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.879332746585688E-5	0.0	0.0	0.12220845089954402	0.0
2	7.879332746585688E-5	0.0	0.0	0.7750111689541683	0.0
3	7.879332746585688E-5	0.0	0.0	1.115949896898931	0.0
4	7.879332746585688E-5	0.0	0.0	1.4873816425729804	0.0
5	7.879332746585688E-5	0.0	0.0	2.4469267844521854	0.0
6	7.879332746585688E-5	0.0	0.0	2.934893861448237	0.0
7	7.879332746585688E-5	0.0	0.0	3.4675367551174294	0.0
8	7.879332746585688E-5	0.0	0.0	4.242075164106803	0.0
9	7.879332746585688E-5	0.0	0.0	4.542908088371444	0.0
10	7.879332746585688E-5	0.0	0.0	5.200911166038815	0.0
11	7.879332746585688E-5	0.0	0.0	6.165105114238506	0.0
12	7.879332746585688E-5	0.0	0.0	6.962729968175375	0.0
13	7.879332746585688E-5	0.0	0.0	7.266320658901321	0.0
14	7.879332746585688E-5	0.0	0.0	7.393020329466419	0.0
15	7.879332746585688E-5	0.0	0.0	7.641534484293732	0.0
16	7.879332746585688E-5	0.0	0.0	8.149830239775975	0.0
17	7.879332746585688E-5	0.0	0.0	8.75449023474896	0.0
18	7.879332746585688E-5	0.0	0.0	9.447950309775967	0.0
19	7.879332746585688E-5	0.0	0.0	9.83908038731648	0.0
20	7.879332746585688E-5	0.0	0.0	10.242974983906462	0.0
21	7.879332746585688E-5	0.0	0.0	10.737560700409647	0.0
22	7.879332746585688E-5	0.0	0.0	11.25554803517019	0.0
23	7.879332746585688E-5	0.0	0.0	11.780311596092796	0.0
24	7.879332746585688E-5	0.0	0.0	12.196576745094918	0.0
25	7.879332746585688E-5	0.0	0.0	12.571317810522533	0.0
26	7.879332746585688E-5	0.0	0.0	12.910129118625719	0.0
27	7.879332746585688E-5	0.0	0.0	13.233890901182924	0.0
28	1.5758665493171377E-4	0.0	0.0	13.578138948881254	0.0
29	1.5758665493171377E-4	0.0	0.0	13.931763402548018	0.0
30	1.5758665493171377E-4	0.0	0.0	14.327463493081552	0.0
31	2.3637998239757065E-4	0.0	0.0	14.706065431554995	0.0
32	2.3637998239757065E-4	0.0	0.0	15.077891143866372	0.0
33	2.3637998239757065E-4	0.0	0.0	15.426000064610529	0.0
34	2.3637998239757065E-4	0.0	0.0	15.756932039967127	0.0
35	2.3637998239757065E-4	0.0	0.0	16.13285500530673	0.0
36	3.1517330986342753E-4	0.0	0.0	16.511772117090036	0.0
37	3.1517330986342753E-4	0.0	0.0	16.871857623609003	0.0
38	3.1517330986342753E-4	0.0	0.0	17.21689360458199	0.0
39	3.1517330986342753E-4	0.0	0.0	17.568627018389574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	30	2.1656815E-6	45.000004	1
CTTCGAA	20	7.033565E-4	45.0	14
CGGTTAG	35	1.2120654E-7	45.0	1
GCGATAC	25	3.891036E-5	45.0	9
GCTAACG	40	6.8157533E-9	45.0	1
GCTCGAA	20	7.033565E-4	45.0	9
CCGTTCA	20	7.033565E-4	45.0	27
CGTTTTT	35560	0.0	44.50647	1
ACGGGTC	110	0.0	42.954544	5
CCCTCGT	95	0.0	42.63158	14
GTTTTTT	39535	0.0	40.264957	2
TAGGGAC	520	0.0	40.240383	5
CGGTAGT	120	0.0	39.375004	12
CGCGACC	115	0.0	39.130436	10
TACGGGT	105	0.0	38.57143	4
GTATTCG	35	6.248805E-6	38.571426	1
TATAGCG	35	6.248805E-6	38.571426	1
GCCCGAA	35	6.248805E-6	38.571426	25
GCTACGA	130	0.0	38.07692	10
GGCACGT	60	1.5643309E-10	37.500004	8
>>END_MODULE