##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1588702
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70445873423713	34.0	31.0	34.0	31.0	34.0
2	32.867324394379814	34.0	31.0	34.0	31.0	34.0
3	32.88491485502001	34.0	31.0	34.0	31.0	34.0
4	36.27102439601637	37.0	37.0	37.0	35.0	37.0
5	36.17234572625955	37.0	37.0	37.0	35.0	37.0
6	36.162509394461644	37.0	37.0	37.0	35.0	37.0
7	36.28017274479418	37.0	37.0	37.0	35.0	37.0
8	36.283463481508804	37.0	37.0	37.0	35.0	37.0
9	38.055865731899374	39.0	39.0	39.0	37.0	39.0
10	37.71646727957792	39.0	38.0	39.0	35.0	39.0
11	37.63307530298319	39.0	37.0	39.0	35.0	39.0
12	37.55080436733887	39.0	37.0	39.0	35.0	39.0
13	37.521740389324115	39.0	37.0	39.0	35.0	39.0
14	38.86305424176466	40.0	38.0	41.0	35.0	41.0
15	38.897756155654115	41.0	38.0	41.0	35.0	41.0
16	38.94979234620464	41.0	38.0	41.0	35.0	41.0
17	38.90298306416181	41.0	38.0	41.0	35.0	41.0
18	38.307206763760604	39.0	38.0	40.0	35.0	41.0
19	37.721775386447554	37.0	37.0	40.0	35.0	41.0
20	37.067912673364795	37.0	35.0	40.0	34.0	41.0
21	37.002886003794295	37.0	35.0	40.0	34.0	41.0
22	36.802546355452435	37.0	35.0	40.0	34.0	41.0
23	36.71972087905724	36.0	35.0	40.0	33.0	41.0
24	36.53672809626978	36.0	35.0	40.0	33.0	41.0
25	36.37332803760554	35.0	35.0	40.0	33.0	41.0
26	36.482666352783596	36.0	35.0	40.0	33.0	41.0
27	36.5111065511342	36.0	35.0	40.0	33.0	41.0
28	36.4153988602016	36.0	35.0	40.0	33.0	41.0
29	36.256851190468694	36.0	35.0	40.0	33.0	41.0
30	35.89567584103249	36.0	35.0	40.0	32.0	41.0
31	35.28626136304984	36.0	35.0	40.0	30.0	41.0
32	34.41609754378102	36.0	35.0	40.0	21.0	41.0
33	33.26692545235041	36.0	33.0	40.0	15.0	41.0
34	32.27785450008875	36.0	31.0	40.0	10.0	41.0
35	31.721825742020844	35.0	30.0	40.0	8.0	41.0
36	31.46934163864589	35.0	29.0	40.0	7.0	41.0
37	31.192222329927198	35.0	25.0	40.0	7.0	41.0
38	31.02440168137259	35.0	24.0	40.0	7.0	41.0
39	30.956305839609946	35.0	23.0	40.0	7.0	41.0
40	30.818422208822046	35.0	23.0	40.0	7.0	41.0
41	30.65634839006938	35.0	23.0	40.0	7.0	41.0
42	30.489420923496038	35.0	23.0	40.0	7.0	41.0
43	30.350377226188424	35.0	22.0	40.0	7.0	41.0
44	30.20929664594115	35.0	22.0	40.0	7.0	41.0
45	30.172453990742127	35.0	22.0	40.0	7.0	41.0
46	30.129683225677315	35.0	23.0	40.0	7.0	41.0
47	30.127169223680717	35.0	23.0	40.0	7.0	41.0
48	30.077013184347976	35.0	23.0	40.0	7.0	41.0
49	29.93741242851082	35.0	22.0	40.0	7.0	41.0
50	29.846344374212407	35.0	22.0	40.0	7.0	41.0
51	29.330881436543795	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	4.0
9	6.0
10	12.0
11	15.0
12	27.0
13	24.0
14	42.0
15	81.0
16	160.0
17	316.0
18	629.0
19	1188.0
20	1977.0
21	3133.0
22	5112.0
23	8413.0
24	17178.0
25	37723.0
26	70574.0
27	93179.0
28	89008.0
29	72202.0
30	56540.0
31	47576.0
32	43213.0
33	48183.0
34	85796.0
35	106243.0
36	110103.0
37	128584.0
38	232902.0
39	328309.0
40	246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.280450329891952	15.207886689888978	17.64031265775457	42.8713503224645
2	55.069925007962475	17.242440684281885	15.579384931850026	12.108249375905613
3	21.402692260726052	16.713581275783625	49.51570527386508	12.368021189625242
4	20.462238985033064	17.092066353538925	49.200857051857426	13.244837609570581
5	17.648243660548044	19.94798269278946	48.514699421288576	13.889074225373921
6	18.483579676994175	22.501954425688393	48.12803156287334	10.886434334444093
7	54.30999646252097	4.4883181364409435	37.56425056429714	3.637434836740937
8	54.958513302054136	4.018626526560676	37.54077227824979	3.482087893135402
9	50.21753607662104	5.914199138667919	38.916108873785014	4.9521559109260265
10	28.436169904739845	18.330246956320316	43.559018620232116	9.674564518707724
11	22.832790542216223	16.64490886270679	47.79650305721274	12.725797537864244
12	21.8312811339068	14.192277721057819	50.46509666381738	13.511344481218002
13	17.627723764431593	16.127568291599054	50.66802962418377	15.576678319785586
14	13.828206926157327	18.373426860418128	51.558882660184224	16.23948355324032
15	14.163512099814819	17.629549153963424	53.987720793452766	14.21921795276899
16	16.148843521314884	16.924445238943488	52.86749812110767	14.059213118633954
17	15.468036170408295	16.993747096686477	51.0483400914709	16.48987664143433
18	16.599337068877613	18.068523864135628	50.29904916088731	15.033089906099445
19	17.171816992739984	19.38639216165146	49.19399610499641	14.247794740612147
20	19.362976820070724	17.946726321235825	49.429660188002536	13.260636670690918
21	17.49956883040369	18.7657597208287	49.58053807447841	14.154133374289199
22	16.37273698906403	17.761166033655147	50.03996973630045	15.826127240980373
23	17.51285011285943	17.8057936604851	50.11915387530198	14.562202351353495
24	17.759592421989776	15.69192963815744	50.74305942838871	15.805418511464076
25	15.863579198616229	17.765635090784805	49.16630053968586	17.204485170913113
26	14.983993222139835	18.92563866603051	49.982375549347836	16.107992562481822
27	15.489940844790276	18.928722944894638	49.55542323229907	16.025912978016017
28	14.179751772201458	20.007213435874068	49.97444454655436	15.83859024537012
29	15.915382494640278	18.092883372715587	49.45232019598389	16.539413936660242
30	16.771741962935778	17.410250632277165	49.535721614248615	16.282285790538438
31	16.94918241432314	19.523170487605604	46.82180799168126	16.70583910639
32	18.459723724147135	20.66617905686529	44.39529880367747	16.47879841531011
33	18.741022545449052	22.038305484603153	42.80425152105304	16.41642044889476
34	16.87314549865236	23.282276978313114	41.83062651145401	18.013951011580524
35	17.153626041888284	25.337917369022005	38.07668146700892	19.431775122080793
36	17.89674841474361	27.79797595773153	36.60950889468258	17.695766732842284
37	18.938982892952865	27.159719066256606	37.000834643627314	16.900463397163218
38	18.20039252169381	27.13164583414637	36.04061680541725	18.62734483874257
39	19.653780255831492	24.599893497962487	36.95110851500156	18.795217731204467
40	19.535948214328428	24.486719346988924	37.37887911011631	18.59845332856634
41	16.68632632173938	27.009092957647184	36.426969941499415	19.87761077911402
42	17.721070408421465	26.301974819695573	36.28811444814698	19.68884032373598
43	18.853819029622926	25.62456646998619	35.827738619325714	19.69387588106517
44	17.98014983300833	25.57735812002503	35.518744232713246	20.923747814253396
45	18.643395677729366	24.73900076918138	35.321287440942356	21.296316112146897
46	17.65875538647273	26.23670140781594	35.68296634611148	20.42157685959985
47	16.82354525895983	25.13045240705935	38.30265210215635	19.743350231824472
48	16.953273804653108	23.061153067095024	39.5740673833104	20.41150574494147
49	17.533747675775572	23.256155024667937	39.229005817327604	19.981091482228887
50	16.016219530157326	23.03981489291258	39.10758594122749	21.836379635702606
51	15.572586929455618	22.732016451165794	38.91988554178191	22.77551107759668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	518.0
1	1061.0
2	1604.0
3	85663.0
4	169722.0
5	115252.5
6	60783.0
7	56833.0
8	52883.0
9	50395.5
10	47908.0
11	44682.5
12	41457.0
13	38326.0
14	35195.0
15	32381.5
16	29568.0
17	27094.5
18	24621.0
19	22599.0
20	20577.0
21	18421.0
22	16265.0
23	15472.5
24	14680.0
25	14279.0
26	14584.5
27	15291.0
28	16838.5
29	18386.0
30	19513.5
31	20641.0
32	22656.5
33	24672.0
34	27656.5
35	30641.0
36	31827.5
37	33014.0
38	34777.0
39	36540.0
40	39782.0
41	43024.0
42	46661.0
43	50298.0
44	53631.5
45	56965.0
46	60619.5
47	64274.0
48	70103.0
49	75932.0
50	83241.5
51	90551.0
52	91153.5
53	91756.0
54	81561.0
55	71366.0
56	67611.0
57	63856.0
58	62495.5
59	61135.0
60	61459.0
61	61783.0
62	56890.0
63	51997.0
64	43036.0
65	34075.0
66	28217.0
67	22359.0
68	18631.0
69	14903.0
70	12156.0
71	9409.0
72	8024.5
73	6640.0
74	5615.0
75	3564.5
76	2539.0
77	1959.0
78	1379.0
79	937.0
80	495.0
81	410.0
82	325.0
83	222.5
84	120.0
85	85.5
86	51.0
87	32.0
88	13.0
89	15.5
90	18.0
91	10.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1588702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.39598152111575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66584268706983	25.484571860333133
2	9.113234868769462	5.904643768124865
3	3.370299797099272	3.275525098423446
4	1.6146565102933021	2.0923352988164425
5	0.9494946685376896	1.537990586817246
6	0.6024337466362648	1.170985951423498
7	0.44346981976591904	1.0056648060416473
8	0.33243192660518356	0.8615566841064348
9	0.26655254149872754	0.7771708087919293
>10	3.918408707711804	31.55520189243396
>50	0.6226434415288937	12.798295392669626
>100	0.09101353067021963	5.835791517948273
>500	0.0061468826711870185	1.3015674627390403
>1k	0.0031725846044836224	1.6023887255959608
>5k	0.0	0.0
>10k+	1.982865377802264E-4	4.7963101457345365
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74666	4.6998115442669555	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	3309	0.20828324002865234	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2111	0.13287576902402087	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1749	0.11008987210943273	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1703	0.10719442664514804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	1680	0.10574670391300571	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT	1622	0.10209592484934243	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.776667996893061E-4	0.0	0.0	0.07792524966922683	0.0
2	3.776667996893061E-4	0.0	0.0	0.5131232918445372	0.0
3	3.776667996893061E-4	0.0	0.0	0.79731755861074	0.0
4	3.776667996893061E-4	0.0	0.0	1.0811971030438685	0.0
5	3.776667996893061E-4	0.0	0.0	1.8180250292377047	0.0
6	3.776667996893061E-4	0.0	0.0	2.252782460146711	0.0
7	3.776667996893061E-4	0.0	0.0	2.6986810616465515	0.0
8	3.776667996893061E-4	0.0	0.0	3.3203835584017645	0.0
9	3.776667996893061E-4	0.0	0.0	3.5690142015305577	0.0
10	3.776667996893061E-4	0.0	0.0	4.047203314403834	0.0
11	3.776667996893061E-4	0.0	0.0	4.843199039215661	0.0
12	3.776667996893061E-4	0.0	0.0	5.471007149232518	0.0
13	3.776667996893061E-4	0.0	0.0	5.712588012100444	0.0
14	3.776667996893061E-4	0.0	0.0	5.815187492682706	0.0
15	4.406112663041905E-4	0.0	0.0	6.0013142804629185	0.0
16	4.406112663041905E-4	0.0	0.0	6.405165978264017	0.0
17	4.406112663041905E-4	0.0	0.0	6.873095143079067	0.0
18	5.035557329190749E-4	0.0	0.0	7.397296661047824	0.0
19	5.035557329190749E-4	0.0	0.0	7.720201774782181	6.294446661488436E-5
20	5.035557329190749E-4	0.0	0.0	8.043610444249456	6.294446661488436E-5
21	5.035557329190749E-4	0.0	0.0	8.423354411337053	6.294446661488436E-5
22	5.035557329190749E-4	0.0	0.0	8.841557447526345	6.294446661488436E-5
23	5.035557329190749E-4	0.0	0.0	9.22514102707745	6.294446661488436E-5
24	5.035557329190749E-4	0.0	0.0	9.543891806015225	6.294446661488436E-5
25	5.035557329190749E-4	0.0	0.0	9.848291246564806	6.294446661488436E-5
26	5.035557329190749E-4	0.0	0.0	10.108314838150893	6.294446661488436E-5
27	5.035557329190749E-4	0.0	0.0	10.376835932729989	6.294446661488436E-5
28	5.035557329190749E-4	0.0	0.0	10.659456587830821	6.294446661488436E-5
29	5.035557329190749E-4	0.0	0.0	10.936664018802771	6.294446661488436E-5
30	5.035557329190749E-4	0.0	0.0	11.257617854072066	6.294446661488436E-5
31	5.035557329190749E-4	0.0	0.0	11.569759464015277	6.294446661488436E-5
32	5.035557329190749E-4	0.0	0.0	11.888258465086592	6.294446661488436E-5
33	5.035557329190749E-4	0.0	0.0	12.195679240033687	6.294446661488436E-5
34	5.035557329190749E-4	0.0	0.0	12.490007565924888	6.294446661488436E-5
35	5.035557329190749E-4	0.0	0.0	12.796043562606455	6.294446661488436E-5
36	5.035557329190749E-4	0.0	0.0	13.112843063079168	6.294446661488436E-5
37	5.035557329190749E-4	0.0	0.0	13.442168512408243	6.294446661488436E-5
38	5.035557329190749E-4	0.0	0.0	13.780369131530016	6.294446661488436E-5
39	5.035557329190749E-4	0.0	0.0	14.103274245264373	6.294446661488436E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	20	7.034103E-4	45.0	43
TACGCTA	20	7.034103E-4	45.0	24
TACGCGC	20	7.034103E-4	45.0	12
TCGTAAG	20	7.034103E-4	45.0	1
TATAACG	20	7.034103E-4	45.0	1
CGTTTTT	51715	0.0	44.534466	1
AATACGG	80	0.0	42.1875	2
TATAGCG	60	3.6379788E-12	41.249996	1
TAGACGG	105	0.0	40.714287	2
GTTTTTT	57340	0.0	40.3658	2
TCATGCG	45	1.9290383E-8	40.0	1
ATTAGCG	40	3.4593359E-7	39.375	1
CATACGG	40	3.4593359E-7	39.375	2
GCGATAC	80	0.0	39.375	9
CATCGGA	40	3.4593359E-7	39.375	27
TAATACG	35	6.2497966E-6	38.57143	1
ACTAGCG	35	6.2497966E-6	38.57143	1
TATTCGA	35	6.2497966E-6	38.57143	19
CGTACTG	35	6.2497966E-6	38.57143	1
TCGATGG	90	0.0	37.5	2
>>END_MODULE