SRR3549861_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438387
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17246	3.933967020007436	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1548	0.3531126607312717	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1443	0.3291612205653909	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	928	0.2116851092755944	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	602	0.13732159028438343	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	569	0.12979399480367804	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	563	0.1284253410799134	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	446	0.10173659346650334	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	30	2.1624164E-6	45.000004	8
GGGTATC	35	1.209628E-7	45.000004	7
ATAGAAC	35	1.209628E-7	45.000004	37
GTTGACG	25	3.8868442E-5	45.0	1
TCGCACC	20	7.0285064E-4	45.0	13
AAATCGA	20	7.0285064E-4	45.0	20
ACACGGC	20	7.0285064E-4	45.0	43
CTCCGTC	20	7.0285064E-4	45.0	14
GCGAAGA	25	3.8868442E-5	45.0	11
AACCCCG	25	3.8868442E-5	45.0	45
GACGTGA	20	7.0285064E-4	45.0	13
CGGTTGA	20	7.0285064E-4	45.0	29
ATGTGCG	20	7.0285064E-4	45.0	1
GACGTAA	20	7.0285064E-4	45.0	11
ACTCTCG	20	7.0285064E-4	45.0	25
TTGTCGC	20	7.0285064E-4	45.0	38
CGCGCTC	20	7.0285064E-4	45.0	22
CGATTTC	25	3.8868442E-5	45.0	10
GCGTTAT	20	7.0285064E-4	45.0	17
GCTTGCG	25	3.8868442E-5	45.0	1