##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438387
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54142800767359	34.0	31.0	34.0	31.0	34.0
2	32.71339706697507	34.0	31.0	34.0	31.0	34.0
3	32.75579111606868	34.0	31.0	34.0	31.0	34.0
4	36.16676589406164	37.0	37.0	37.0	35.0	37.0
5	36.049923925663855	37.0	35.0	37.0	35.0	37.0
6	36.04249897921243	37.0	35.0	37.0	35.0	37.0
7	36.18533624400359	37.0	37.0	37.0	35.0	37.0
8	36.17837207763916	37.0	37.0	37.0	35.0	37.0
9	37.95024943714115	39.0	39.0	39.0	35.0	39.0
10	37.57853677230392	39.0	38.0	39.0	35.0	39.0
11	37.49250776140716	39.0	37.0	39.0	35.0	39.0
12	37.36090942477765	39.0	37.0	39.0	35.0	39.0
13	37.27030682935397	39.0	37.0	39.0	34.0	39.0
14	38.516495699005674	40.0	38.0	41.0	34.0	41.0
15	38.59219365537756	40.0	38.0	41.0	34.0	41.0
16	38.69156247790195	40.0	38.0	41.0	35.0	41.0
17	38.70581016316633	40.0	38.0	41.0	35.0	41.0
18	38.23180660010448	39.0	38.0	41.0	35.0	41.0
19	37.74460693405598	38.0	37.0	41.0	35.0	41.0
20	37.190744707301995	38.0	35.0	40.0	34.0	41.0
21	37.094580815580755	38.0	35.0	40.0	34.0	41.0
22	36.90006546726978	38.0	35.0	40.0	33.0	41.0
23	36.76467824091499	37.0	35.0	40.0	33.0	41.0
24	36.57529534406814	37.0	35.0	40.0	33.0	41.0
25	36.41528375613328	37.0	35.0	40.0	33.0	41.0
26	36.535134481633804	37.0	35.0	40.0	33.0	41.0
27	36.58418246891445	37.0	35.0	40.0	33.0	41.0
28	36.534621236487396	37.0	35.0	40.0	33.0	41.0
29	36.41283614705728	37.0	35.0	40.0	33.0	41.0
30	36.091968968057905	37.0	35.0	40.0	32.0	41.0
31	35.564211529995184	37.0	35.0	40.0	30.0	41.0
32	34.84898731029889	37.0	35.0	40.0	23.0	41.0
33	33.92053368370868	37.0	34.0	40.0	17.0	41.0
34	33.07699361523038	37.0	33.0	40.0	12.0	41.0
35	32.59654141204005	37.0	33.0	40.0	10.0	41.0
36	32.34881736912819	37.0	32.0	40.0	8.0	41.0
37	32.09293843111224	36.0	31.0	40.0	8.0	41.0
38	31.96279771069854	36.0	30.0	40.0	8.0	41.0
39	31.880256485707832	36.0	30.0	40.0	8.0	41.0
40	31.693763729307665	36.0	30.0	40.0	8.0	41.0
41	31.57036590957305	36.0	29.0	40.0	7.0	41.0
42	31.428057857555082	36.0	29.0	40.0	7.0	41.0
43	31.264850463175232	35.0	28.0	40.0	7.0	41.0
44	31.131034907513225	35.0	27.0	40.0	7.0	41.0
45	31.104357565347513	35.0	26.0	40.0	7.0	41.0
46	31.06192473773173	35.0	26.0	40.0	7.0	41.0
47	31.045199789227325	35.0	26.0	40.0	7.0	41.0
48	31.00557954501388	35.0	26.0	40.0	7.0	41.0
49	30.86997789624236	35.0	26.0	40.0	7.0	41.0
50	30.745361974693594	35.0	25.0	40.0	7.0	41.0
51	30.230253178128002	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	8.0
12	7.0
13	13.0
14	12.0
15	34.0
16	51.0
17	92.0
18	218.0
19	358.0
20	614.0
21	1033.0
22	1627.0
23	2628.0
24	4899.0
25	10083.0
26	18033.0
27	22445.0
28	20035.0
29	15758.0
30	12222.0
31	10865.0
32	10944.0
33	13476.0
34	24465.0
35	36766.0
36	31966.0
37	39952.0
38	71354.0
39	88359.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.156202168403716	15.9477812982593	18.594757600020074	36.30125893331691
2	49.87351358502875	19.089297812207022	17.909062084414	13.128126518350225
3	22.397105753592143	18.904985777406722	46.051319952462094	12.64658851653904
4	21.184250445382734	19.41458118055508	45.75614696603686	13.645021408025329
5	19.282049878303873	21.685177708280584	44.46527839557286	14.567494017842684
6	19.51494912029782	25.119586119113933	43.388832241831985	11.976632518756258
7	58.433758300314565	5.092304288220226	32.37299463715849	4.10094277430672
8	58.44060156893338	4.850965014929731	31.899896666643855	4.808536749493028
9	53.71623702345188	6.64298895724554	33.806659412801935	5.834114606500649
10	31.112692666525238	20.034809426374416	38.41651326339513	10.435984643705218
11	25.48410422754324	18.956766509955816	41.7688024508026	13.790326811698339
12	23.31684105596197	16.918156788408414	45.382960717357044	14.382041438272578
13	18.261490418283387	21.07065218631027	46.20415295161581	14.463704443790531
14	13.223704170059788	24.341050259245826	45.360606039868884	17.074639530825504
15	12.991489254927725	19.262660617217207	52.76046050635625	14.985389621498813
16	13.854881645669238	18.435765659109418	51.523425649027	16.185927046194344
17	13.976235609176365	18.365964319197424	46.58874464799367	21.069055423632545
18	15.350820165743967	19.800997748564626	46.783093476768244	18.065088608923165
19	18.228186511005116	19.738267786225414	44.771172502834254	17.262373199935215
20	20.544632938476735	19.437163966997197	44.42558743758369	15.592615656942382
21	17.845191577304984	21.146156250071286	44.898913517052286	16.109738655571448
22	16.490452499731973	19.827458387224073	43.56972264232288	20.112366470721074
23	16.030128630639137	21.4112188545737	44.73216587170696	17.8264866430802
24	17.64263082618782	18.75671495733222	46.402835850515636	17.197818365964316
25	15.537869507991797	20.277289244434712	45.151658238040824	19.03318300953267
26	14.846015050628782	21.627466142928508	45.26959056723854	18.25692823920417
27	16.094683464610036	21.806531671787713	45.51914176287162	16.57964310073063
28	14.487655883956412	21.416921578422716	47.74480082666685	16.35062171095402
29	16.0896650676229	19.081313998818395	46.302696019726866	18.526324913831843
30	16.443690164170015	20.805133363899934	44.69566843907324	18.055508032856814
31	17.357266524782897	23.277150097972797	41.185984073432834	18.17959930381147
32	19.837951399106267	22.876134556909765	39.93503456991197	17.350879474071995
33	19.916420879268774	24.088305538257295	37.79628501757579	18.198988564898137
34	18.106832547497987	23.92680439885307	38.854710563953766	19.111652489695178
35	19.22502263981368	23.392117010769024	35.58454059997217	21.798319749445124
36	20.524787459482148	25.692595811463388	35.556711307588955	18.22590542146551
37	20.531630728100968	26.30438402598617	36.58206105564262	16.581924190270243
38	20.790762499800405	26.45128619233691	33.8579839274431	18.899967380419582
39	21.74608279898811	25.483876118589283	33.92618850467738	18.843852577745235
40	21.24196201073481	25.376436801273762	34.892458033655195	18.489143154336237
41	18.87761270293143	25.360925392404425	33.51399562487026	22.247466279793883
42	21.18949695132383	23.961476959855105	33.70651958201315	21.142506506807912
43	21.361947320518173	24.5290120373095	34.35913929929492	19.74990134287741
44	19.36964371662481	25.66727571757374	33.456512168472145	21.5065683973293
45	19.575626102051384	24.91086642623983	32.882818149260814	22.630689322447974
46	19.92668578219701	25.85911534785475	33.49278149215191	20.72141737779633
47	18.55210122562941	24.924324854523512	36.074518633079904	20.44905528676717
48	19.693786540203064	22.03053466457719	35.60438607896676	22.671292716252992
49	18.997598012714793	22.355361815017325	36.97395223854722	21.673087933720662
50	18.1643160038961	22.89369894636474	36.38953709849973	22.55244795123943
51	17.716538127271107	23.738614511835433	33.20604853702322	25.338798823870235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	286.0
1	375.5
2	465.0
3	16174.5
4	31884.0
5	21897.0
6	11910.0
7	11554.5
8	11199.0
9	11098.5
10	10998.0
11	10497.5
12	9997.0
13	9450.0
14	8903.0
15	8193.5
16	7484.0
17	7050.5
18	6617.0
19	6143.0
20	5669.0
21	5247.5
22	4826.0
23	4523.5
24	4221.0
25	4144.0
26	4044.5
27	4022.0
28	4600.0
29	5178.0
30	5555.5
31	5933.0
32	5841.5
33	5750.0
34	6664.5
35	7579.0
36	7738.5
37	7898.0
38	8829.0
39	9760.0
40	11098.0
41	12436.0
42	16090.0
43	19744.0
44	21362.5
45	22981.0
46	26180.5
47	29380.0
48	32569.5
49	35759.0
50	34302.5
51	32846.0
52	27924.5
53	23003.0
54	20210.5
55	17418.0
56	16070.0
57	14722.0
58	14311.5
59	13901.0
60	14192.0
61	14483.0
62	13346.5
63	12210.0
64	10096.0
65	7982.0
66	6797.5
67	5613.0
68	4767.0
69	3921.0
70	3345.0
71	2769.0
72	2200.0
73	1631.0
74	1373.0
75	1010.0
76	905.0
77	645.5
78	386.0
79	362.0
80	338.0
81	226.5
82	115.0
83	75.5
84	36.0
85	40.0
86	44.0
87	22.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	438387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.81081559773134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34432501578536	30.378844574960535
2	8.713453226086992	6.589255463020651
3	2.9815360949326735	3.3820293445043794
4	1.4767843185379952	2.2335367818344594
5	0.8240188190107084	1.5578411807337127
6	0.5490228838249794	1.2455401811544584
7	0.36437633939184744	0.964415660384506
8	0.32871128161751617	0.9943073323307072
9	0.2606167837864585	0.8868719838081238
>10	3.7370027644821953	34.98021087946556
>50	0.37441744287604833	8.672217761874592
>100	0.041466426898324575	2.8350949058813675
>500	0.002439201582254387	0.6137786792010572
>1k	0.0012196007911271934	0.6896363747146363
>5k	0.0	0.0
>10k+	6.098003955635967E-4	3.9764188961312668
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17246	3.933967020007436	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1548	0.3531126607312717	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1443	0.3291612205653909	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	928	0.2116851092755944	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	602	0.13732159028438343	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	569	0.12979399480367804	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	563	0.1284253410799134	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	446	0.10173659346650334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.281089539607698E-4	0.0	0.0	0.2237748838355152	0.0
2	2.281089539607698E-4	0.0	0.0	1.21490828879506	0.0
3	2.281089539607698E-4	0.0	0.0	1.7274691083449099	0.0
4	2.281089539607698E-4	0.0	0.0	2.2370645114932697	0.0
5	2.281089539607698E-4	0.0	0.0	3.5651148414528717	0.0
6	2.281089539607698E-4	0.0	0.0	4.290273206094159	0.0
7	2.281089539607698E-4	0.0	0.0	5.0656155406068155	0.0
8	2.281089539607698E-4	0.0	0.0	6.359221418518341	0.0
9	2.281089539607698E-4	0.0	0.0	6.882959576812269	0.0
10	2.281089539607698E-4	0.0	0.0	7.8816205772525185	0.0
11	2.281089539607698E-4	0.0	0.0	9.153784213491733	0.0
12	2.281089539607698E-4	0.0	0.0	10.263306165556916	0.0
13	2.281089539607698E-4	0.0	0.0	10.68850125573979	0.0
14	2.281089539607698E-4	0.0	0.0	10.854336465269272	0.0
15	2.281089539607698E-4	0.0	0.0	11.163652206840075	0.0
16	2.281089539607698E-4	0.0	0.0	11.768597152744036	0.0
17	2.281089539607698E-4	0.0	0.0	12.494667953201168	0.0
18	2.281089539607698E-4	0.0	0.0	13.474395910462674	0.0
19	2.281089539607698E-4	0.0	0.0	13.93905384968076	0.0
20	4.562179079215396E-4	0.0	0.0	14.364248939863636	0.0
21	4.562179079215396E-4	0.0	0.0	14.865632420669408	0.0
22	4.562179079215396E-4	0.0	0.0	15.362909940303886	0.0
23	4.562179079215396E-4	0.0	0.0	15.895316238848324	0.0
24	4.562179079215396E-4	0.0	0.0	16.27603008300885	0.0
25	4.562179079215396E-4	0.0	0.0	16.609297264745532	0.0
26	4.562179079215396E-4	0.0	0.0	16.912454064559395	0.0
27	4.562179079215396E-4	0.0	0.0	17.20306487190542	0.0
28	4.562179079215396E-4	0.0	0.0	17.511696286614338	0.0
29	4.562179079215396E-4	0.0	0.0	17.831505040067338	0.0
30	4.562179079215396E-4	0.0	0.0	18.20925346782637	0.0
31	4.562179079215396E-4	0.0	0.0	18.589739203032938	0.0
32	4.562179079215396E-4	0.0	0.0	18.942851863764208	0.0
33	4.562179079215396E-4	0.0	0.0	19.253080041150856	0.0
34	4.562179079215396E-4	0.0	0.0	19.55076222606966	0.0
35	4.562179079215396E-4	0.0	0.0	19.886310497345953	0.0
36	9.124358158430793E-4	0.0	0.0	20.225508511885618	0.0
37	9.124358158430793E-4	0.0	0.0	20.578621172616888	0.0
38	9.124358158430793E-4	0.0	0.0	20.900026688747612	0.0
39	9.124358158430793E-4	0.0	0.0	21.18630342596838	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	30	2.1624164E-6	45.000004	8
GGGTATC	35	1.209628E-7	45.000004	7
ATAGAAC	35	1.209628E-7	45.000004	37
GTTGACG	25	3.8868442E-5	45.0	1
TCGCACC	20	7.0285064E-4	45.0	13
AAATCGA	20	7.0285064E-4	45.0	20
ACACGGC	20	7.0285064E-4	45.0	43
CTCCGTC	20	7.0285064E-4	45.0	14
GCGAAGA	25	3.8868442E-5	45.0	11
AACCCCG	25	3.8868442E-5	45.0	45
GACGTGA	20	7.0285064E-4	45.0	13
CGGTTGA	20	7.0285064E-4	45.0	29
ATGTGCG	20	7.0285064E-4	45.0	1
GACGTAA	20	7.0285064E-4	45.0	11
ACTCTCG	20	7.0285064E-4	45.0	25
TTGTCGC	20	7.0285064E-4	45.0	38
CGCGCTC	20	7.0285064E-4	45.0	22
CGATTTC	25	3.8868442E-5	45.0	10
GCGTTAT	20	7.0285064E-4	45.0	17
GCTTGCG	25	3.8868442E-5	45.0	1
>>END_MODULE