SRR3549859_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1581971
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	6085	0.3846467476331741	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	4590	0.29014438317769414	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3779	0.2388792209212432	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	3121	0.1972855381040487	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2287	0.14456649331751342	TruSeq Adapter, Index 19 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1631	0.10309923506815231	TruSeq Adapter, Index 13 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	25	3.891473E-5	45.000004	42
TATATCG	35	1.21232E-7	45.000004	20
ACGTACG	25	3.891473E-5	45.000004	10
CCGCGCA	25	3.891473E-5	45.000004	14
GCCGTAA	25	3.891473E-5	45.000004	25
AGGTCGC	25	3.891473E-5	45.000004	10
GTCGCAA	20	7.0340926E-4	45.0	38
TAGACGC	20	7.0340926E-4	45.0	35
TTCACGC	20	7.0340926E-4	45.0	31
ATTCGCG	20	7.0340926E-4	45.0	1
CGATCAT	20	7.0340926E-4	45.0	10
TATCGGC	20	7.0340926E-4	45.0	19
TCGGTCG	20	7.0340926E-4	45.0	25
TGCGTAG	155	0.0	43.54839	1
AGTAACG	160	0.0	42.1875	1
TCAATCG	50	1.0822987E-9	40.500004	28
TCGCAAG	45	1.9290383E-8	40.0	1
GCTTCGC	45	1.9290383E-8	40.0	18
CGGTCTA	45	1.9290383E-8	40.0	31
GCGTAAG	130	0.0	39.80769	1