##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1581971
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32179603798047	33.0	31.0	34.0	30.0	34.0
2	32.50011409817247	34.0	31.0	34.0	31.0	34.0
3	32.501353691060075	34.0	31.0	34.0	31.0	34.0
4	36.01774874507814	37.0	35.0	37.0	35.0	37.0
5	35.91826145991298	37.0	35.0	37.0	35.0	37.0
6	35.85805997707923	37.0	35.0	37.0	35.0	37.0
7	36.14075668896585	37.0	35.0	37.0	35.0	37.0
8	36.00053730441329	37.0	35.0	37.0	35.0	37.0
9	37.723569521818035	39.0	38.0	39.0	35.0	39.0
10	37.402559212526654	39.0	37.0	39.0	35.0	39.0
11	37.32243068931099	39.0	37.0	39.0	34.0	39.0
12	37.253136119435815	39.0	37.0	39.0	34.0	39.0
13	37.208946308118165	39.0	37.0	39.0	34.0	39.0
14	38.40338982193732	40.0	38.0	41.0	34.0	41.0
15	38.47020014905456	40.0	38.0	41.0	34.0	41.0
16	38.54720345695338	40.0	38.0	41.0	34.0	41.0
17	38.53537454226405	40.0	38.0	41.0	34.0	41.0
18	38.50480381751625	40.0	38.0	41.0	34.0	41.0
19	38.49419489990651	40.0	38.0	41.0	34.0	41.0
20	38.4532933915982	40.0	37.0	41.0	34.0	41.0
21	38.38990032054949	40.0	37.0	41.0	34.0	41.0
22	38.32865014592556	40.0	37.0	41.0	34.0	41.0
23	38.176764302253325	40.0	37.0	41.0	34.0	41.0
24	37.92957456236555	40.0	36.0	41.0	33.0	41.0
25	37.86787747689433	40.0	36.0	41.0	33.0	41.0
26	38.02641641344879	40.0	37.0	41.0	34.0	41.0
27	38.08577021955523	40.0	37.0	41.0	34.0	41.0
28	38.05551745259553	40.0	37.0	41.0	34.0	41.0
29	38.059368977054575	40.0	37.0	41.0	34.0	41.0
30	37.95438980866274	40.0	37.0	41.0	34.0	41.0
31	37.899969721316005	40.0	37.0	41.0	34.0	41.0
32	37.85464272101069	40.0	37.0	41.0	34.0	41.0
33	37.74890500521185	40.0	36.0	41.0	33.0	41.0
34	37.69394002797776	40.0	36.0	41.0	33.0	41.0
35	37.65058019394793	40.0	36.0	41.0	33.0	41.0
36	37.578848790527765	40.0	36.0	41.0	33.0	41.0
37	37.529792265471364	40.0	36.0	41.0	33.0	41.0
38	37.45216062747041	40.0	36.0	41.0	33.0	41.0
39	37.47069889397467	40.0	36.0	41.0	33.0	41.0
40	37.40398401740613	40.0	36.0	41.0	33.0	41.0
41	37.34911638708927	40.0	36.0	41.0	33.0	41.0
42	37.25305900044944	40.0	35.0	41.0	33.0	41.0
43	37.20417378068245	39.0	35.0	41.0	33.0	41.0
44	37.142376819802635	39.0	35.0	41.0	32.0	41.0
45	37.10947103328696	39.0	35.0	41.0	32.0	41.0
46	37.06912895369131	39.0	35.0	41.0	32.0	41.0
47	36.963616273623224	39.0	35.0	41.0	32.0	41.0
48	36.91044968586655	39.0	35.0	41.0	32.0	41.0
49	36.86206257889683	39.0	35.0	41.0	32.0	41.0
50	36.765389504611655	39.0	35.0	41.0	32.0	41.0
51	35.84957309584057	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	14.0
10	20.0
11	19.0
12	18.0
13	28.0
14	33.0
15	47.0
16	68.0
17	159.0
18	284.0
19	539.0
20	837.0
21	1400.0
22	2130.0
23	3067.0
24	4463.0
25	6650.0
26	8962.0
27	11174.0
28	12852.0
29	15416.0
30	18734.0
31	24122.0
32	32100.0
33	45620.0
34	86287.0
35	119437.0
36	109816.0
37	172530.0
38	335240.0
39	569444.0
40	448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.79105811674171	22.10432428913046	26.36243015832781	11.742187435800023
2	30.50548967079675	24.15202301432833	26.63512795114449	18.707359363730436
3	30.815545923408205	23.93090644518768	28.167836199272934	17.085711432131184
4	28.35924299497273	25.67556548128885	27.42901102485444	18.536180498883986
5	25.102798976719548	29.7426438284899	26.136572667893404	19.017984526897145
6	26.31957222983228	33.923441074457116	24.557529815654018	15.199456880056589
7	82.4011944593169	5.362361256938338	7.840598847892913	4.395845435851858
8	84.51444432293638	3.68091450475388	7.158980790419041	4.645660381890692
9	79.11244896398227	5.627031089697599	9.272799564593788	5.98772038172634
10	38.461071663134156	25.713935337626292	18.061582671237336	17.76341032800222
11	28.702548908924374	27.72345384333847	24.018265821560572	19.555731426176585
12	28.50494730940074	22.953518111267528	28.267711607861333	20.273822971470402
13	24.0357756242055	23.91883289895959	30.154977556478595	21.890413920356313
14	19.40724577125624	28.11043944547656	28.70014684213554	23.782167941131664
15	18.61178239044837	26.40219068491142	33.7709098333661	21.215117091274113
16	20.97244513331787	25.705970589852782	31.95867686575797	21.362907411071376
17	21.498560972356636	24.98945935165689	28.388952768413578	25.123026907572893
18	21.750588348332556	26.790630169579593	29.370260263936572	22.08852121815128
19	23.405359516704163	27.572376484777532	27.168955688821093	21.853308309697205
20	26.728176433069883	26.423746073727017	27.061621230730527	19.786456262472573
21	24.447603653922858	26.326209519643534	28.436930891906364	20.789255934527244
22	22.25565449682706	25.221764495050795	27.037979836545677	25.48460117157647
23	21.570496551453854	26.863071446948144	28.57005596183495	22.996376039763057
24	22.947639368863275	24.508603507902485	29.493081731586734	23.050675391647506
25	21.191412484805348	26.32962298297504	27.89671871355417	24.58224581866545
26	20.91308879872008	28.209366669806208	28.07263849969437	22.804906031779343
27	22.162985288605164	28.401595225196925	28.356651291332142	21.07876819486577
28	19.70516526535569	27.98945113405998	29.85560417984906	22.449779420735272
29	20.8178278868576	26.32513491081695	29.52140083478142	23.335636367544033
30	22.178788359584342	27.437671107751026	27.782746965652343	22.60079356701229
31	21.888580764122732	28.68535516769903	25.592694177074044	23.833369891104198
32	22.77431128636366	27.22369752669297	27.00296023125582	22.99903095568756
33	23.205229425823862	28.190529409199033	25.466079972388872	23.138161192588232
34	20.129825388708138	27.0759072068957	27.94855278636587	24.845714618030293
35	20.97276119473745	24.278068308458245	29.324873844084376	25.42429665271993
36	23.180386998244597	27.2953170443706	26.976916770282134	22.54737918710267
37	21.844458589948868	27.195568060350034	28.21910136152938	22.74087198817172
38	20.87882774083722	27.644375276158666	27.28400204554951	24.192794937454607
39	22.055208344527173	25.876327695008317	27.41832814887251	24.650135811591994
40	22.272216115213237	24.205437394237947	30.902336389225844	22.620010101322972
41	20.126538349944468	25.791243960856423	29.942331433382783	24.13988625581632
42	22.75888748908798	25.993839330809475	28.33819330442846	22.90907987567408
43	23.507636992081395	25.97171503143863	27.349679608538967	23.170968367941004
44	21.54186138683958	26.59878088789238	26.803082989511186	25.05627473575685
45	21.15310584075182	24.740782226728555	26.943098198386693	27.163013734132928
46	21.300074400858172	26.655039820578253	28.046595038720685	23.998290739842894
47	20.430715860151672	25.314876189260104	29.83234205936771	24.42206589122051
48	20.800823782484002	24.276740850495994	29.638722833730835	25.283712533289172
49	21.437624330660928	24.377374806491396	30.18968110034887	23.995319762498806
50	19.877924437300052	24.49330613519464	29.463688019565463	26.165081407939844
51	19.709021214674603	24.04696419845876	27.885846200720493	28.358168386146144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	591.0
1	877.5
2	1164.0
3	4200.0
4	7236.0
5	5025.5
6	2815.0
7	2763.5
8	2712.0
9	2675.5
10	2639.0
11	2639.0
12	2639.0
13	2635.5
14	2632.0
15	2717.0
16	2802.0
17	2748.5
18	2695.0
19	3019.0
20	3343.0
21	3660.0
22	3977.0
23	4237.0
24	4497.0
25	5370.0
26	10262.0
27	14281.0
28	17778.5
29	21276.0
30	23440.5
31	25605.0
32	29393.0
33	33181.0
34	35578.5
35	37976.0
36	44365.5
37	50755.0
38	55735.5
39	60716.0
40	65988.0
41	71260.0
42	79937.5
43	88615.0
44	97570.5
45	106526.0
46	123646.5
47	140767.0
48	154527.5
49	168288.0
50	167662.0
51	167036.0
52	143017.0
53	118998.0
54	106211.0
55	93424.0
56	83539.5
57	73655.0
58	69570.5
59	65486.0
60	65566.0
61	65646.0
62	59220.5
63	52795.0
64	43458.0
65	34121.0
66	26497.0
67	18873.0
68	14773.5
69	10674.0
70	8481.5
71	6289.0
72	5202.5
73	4116.0
74	3530.5
75	2019.5
76	1094.0
77	927.0
78	760.0
79	542.0
80	324.0
81	339.5
82	355.0
83	231.5
84	108.0
85	64.5
86	21.0
87	13.0
88	5.0
89	5.0
90	5.0
91	7.0
92	9.0
93	4.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1581971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.050687309572666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.58265951252469	15.12294779769571
2	12.312225624997938	5.4298607468347475
3	5.35129061662303	3.5399890826941416
4	2.8960129377996555	2.554363029435885
5	1.7430985828978813	1.9218260900620203
6	1.1167449608483424	1.4774996361724662
7	0.817114045300083	1.261254841642049
8	0.5816479373496579	1.0260589432604177
9	0.45191871975729025	0.8968606540839367
>10	3.7926180563308223	19.555692249729198
>50	1.754275732440284	28.135379202070375
>100	0.594311141569683	16.980450834542758
>500	0.0034755037489662397	0.4867103790863181
>1k	0.002317002499310827	1.2224918090106678
>5k	2.8962531241385337E-4	0.38861470367933937
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	6085	0.3846467476331741	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	4590	0.29014438317769414	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3779	0.2388792209212432	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	3121	0.1972855381040487	TruSeq Adapter, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2287	0.14456649331751342	TruSeq Adapter, Index 19 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1631	0.10309923506815231	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2642456783341793E-4	0.0	0.0	0.20676738069155504	0.0
2	1.896368517501269E-4	0.0	0.0	1.1780241230717883	0.0
3	1.896368517501269E-4	0.0	0.0	1.7004736496433879	0.0
4	1.896368517501269E-4	0.0	0.0	2.2720391208182704	0.0
5	1.896368517501269E-4	0.0	0.0	3.7863525943269503	0.0
6	1.896368517501269E-4	0.0	0.0	4.584534103343234	0.0
7	1.896368517501269E-4	0.0	0.0	5.399530079881363	0.0
8	1.896368517501269E-4	0.0	0.0	6.674901120184884	0.0
9	2.5284913566683587E-4	0.0	0.0	7.106767443903839	0.0
10	2.5284913566683587E-4	0.0	0.0	8.17872135456339	0.0
11	2.5284913566683587E-4	0.0	0.0	9.772239819819706	0.0
12	2.5284913566683587E-4	0.0	0.0	10.944701261906824	0.0
13	2.5284913566683587E-4	0.0	0.0	11.402042136044214	0.0
14	2.5284913566683587E-4	0.0	0.0	11.574674883420746	0.0
15	2.5284913566683587E-4	0.0	0.0	11.924744511751479	0.0
16	2.5284913566683587E-4	0.0	0.0	12.734177807304938	0.0
17	2.5284913566683587E-4	0.0	0.0	13.676799385070902	0.0
18	2.5284913566683587E-4	0.0	0.0	14.701470507360755	0.0
19	2.5284913566683587E-4	0.0	0.0	15.300975808026822	0.0
20	3.1606141958354485E-4	0.0	0.0	15.913502839179731	0.0
21	3.1606141958354485E-4	0.0	0.0	16.748347472867707	0.0
22	3.1606141958354485E-4	0.0	0.0	17.59899517753486	0.0
23	3.1606141958354485E-4	0.0	0.0	18.444206625785174	0.0
24	3.1606141958354485E-4	0.0	0.0	19.079363654580266	0.0
25	3.1606141958354485E-4	0.0	0.0	19.612938543121206	0.0
26	3.1606141958354485E-4	0.0	0.0	20.108902122731706	0.0
27	3.1606141958354485E-4	0.0	0.0	20.58381601179794	0.0
28	3.1606141958354485E-4	0.0	0.0	21.098300790596035	0.0
29	3.792737035002538E-4	0.0	0.0	21.660131570047746	0.0
30	3.792737035002538E-4	0.0	0.0	22.27392284687899	0.0
31	3.792737035002538E-4	0.0	0.0	22.84435049694337	0.0
32	3.792737035002538E-4	0.0	0.0	23.41446208558817	0.0
33	3.792737035002538E-4	0.0	0.0	23.933561361112183	0.0
34	4.4248598741696276E-4	0.0	0.0	24.46852691989929	0.0
35	4.4248598741696276E-4	0.0	0.0	25.02479501836633	0.0
36	5.056982713336717E-4	0.0	0.0	25.58814289263204	0.0
37	5.056982713336717E-4	0.0	0.0	26.136193394189906	0.0
38	5.689105552503807E-4	0.0	0.0	26.671095740693097	0.0
39	6.321228391670897E-4	0.0	0.0	27.20827372941729	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	25	3.891473E-5	45.000004	42
TATATCG	35	1.21232E-7	45.000004	20
ACGTACG	25	3.891473E-5	45.000004	10
CCGCGCA	25	3.891473E-5	45.000004	14
GCCGTAA	25	3.891473E-5	45.000004	25
AGGTCGC	25	3.891473E-5	45.000004	10
GTCGCAA	20	7.0340926E-4	45.0	38
TAGACGC	20	7.0340926E-4	45.0	35
TTCACGC	20	7.0340926E-4	45.0	31
ATTCGCG	20	7.0340926E-4	45.0	1
CGATCAT	20	7.0340926E-4	45.0	10
TATCGGC	20	7.0340926E-4	45.0	19
TCGGTCG	20	7.0340926E-4	45.0	25
TGCGTAG	155	0.0	43.54839	1
AGTAACG	160	0.0	42.1875	1
TCAATCG	50	1.0822987E-9	40.500004	28
TCGCAAG	45	1.9290383E-8	40.0	1
GCTTCGC	45	1.9290383E-8	40.0	18
CGGTCTA	45	1.9290383E-8	40.0	31
GCGTAAG	130	0.0	39.80769	1
>>END_MODULE