##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808226
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19215170014328	33.0	31.0	34.0	30.0	34.0
2	32.37648256799459	34.0	31.0	34.0	30.0	34.0
3	32.389910742787286	34.0	31.0	34.0	30.0	34.0
4	35.941069700801506	37.0	35.0	37.0	35.0	37.0
5	35.81403840015045	37.0	35.0	37.0	35.0	37.0
6	35.71878781429947	37.0	35.0	37.0	35.0	37.0
7	35.96722080210238	37.0	35.0	37.0	35.0	37.0
8	35.59414569687191	37.0	35.0	37.0	35.0	37.0
9	37.372872191688955	39.0	37.0	39.0	35.0	39.0
10	37.229145065860294	39.0	37.0	39.0	34.0	39.0
11	37.159676872557924	39.0	37.0	39.0	34.0	39.0
12	36.9845612489576	39.0	35.0	39.0	33.0	39.0
13	36.78393295934553	39.0	35.0	39.0	33.0	39.0
14	37.94207560756521	40.0	37.0	41.0	33.0	41.0
15	37.94445736712256	40.0	37.0	41.0	33.0	41.0
16	38.1753247235303	40.0	37.0	41.0	34.0	41.0
17	38.030956687857106	40.0	36.0	41.0	33.0	41.0
18	38.06767290337109	40.0	37.0	41.0	34.0	41.0
19	38.01326732869272	40.0	37.0	41.0	34.0	41.0
20	37.75336972579452	40.0	36.0	41.0	33.0	41.0
21	37.846504566791964	40.0	36.0	41.0	33.0	41.0
22	37.77137335349271	40.0	36.0	41.0	33.0	41.0
23	37.50292863629727	39.0	35.0	41.0	33.0	41.0
24	37.28974816449854	39.0	35.0	41.0	32.0	41.0
25	37.265570521116615	39.0	35.0	41.0	32.0	41.0
26	37.372046927468304	39.0	35.0	41.0	33.0	41.0
27	37.34963240479767	39.0	35.0	41.0	33.0	41.0
28	37.30835805826588	39.0	35.0	41.0	32.0	41.0
29	37.27971879152613	39.0	35.0	41.0	32.0	41.0
30	37.21279567843648	39.0	35.0	41.0	32.0	41.0
31	37.16730468952991	39.0	35.0	41.0	32.0	41.0
32	36.92550845926758	39.0	35.0	41.0	31.0	41.0
33	36.78475079990003	39.0	35.0	41.0	31.0	41.0
34	36.62774026076865	39.0	35.0	41.0	31.0	41.0
35	36.542828367313106	39.0	35.0	41.0	31.0	41.0
36	36.51841440389198	39.0	35.0	41.0	30.0	41.0
37	36.39331696827372	39.0	35.0	41.0	30.0	41.0
38	36.391009445377904	39.0	35.0	41.0	30.0	41.0
39	36.38546272948408	39.0	35.0	41.0	30.0	41.0
40	36.38254151685296	39.0	35.0	41.0	30.0	41.0
41	36.142766998339575	39.0	35.0	41.0	30.0	41.0
42	36.160201478299385	39.0	35.0	41.0	30.0	41.0
43	36.10304296075603	39.0	35.0	41.0	30.0	41.0
44	36.15864622024038	39.0	35.0	41.0	30.0	41.0
45	36.11816744326463	39.0	35.0	41.0	30.0	41.0
46	36.05016171219436	39.0	35.0	40.0	30.0	41.0
47	35.82628002563639	38.0	35.0	40.0	29.0	41.0
48	35.8126477000245	38.0	35.0	40.0	29.0	41.0
49	35.86093369923759	38.0	35.0	40.0	29.0	41.0
50	35.75410466874364	38.0	35.0	40.0	29.0	41.0
51	34.8705708551816	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	15.0
11	15.0
12	14.0
13	10.0
14	17.0
15	23.0
16	47.0
17	98.0
18	229.0
19	486.0
20	981.0
21	1958.0
22	3134.0
23	4291.0
24	5315.0
25	6674.0
26	7791.0
27	8661.0
28	8871.0
29	9884.0
30	12037.0
31	15539.0
32	21052.0
33	28999.0
34	51939.0
35	74039.0
36	59736.0
37	91310.0
38	159945.0
39	234923.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.68134160494713	19.29002531470158	21.364321365558645	11.664311714792644
2	30.621385602541864	31.346554057899645	21.907115089096365	16.124945250462122
3	28.373252035940443	31.647336264856612	24.542516573334687	15.436895125868261
4	26.554948739585214	22.6213955007634	33.69020546233356	17.13345029731783
5	30.947779457725932	27.16962829703573	23.125957343614285	18.75663490162405
6	23.315384558279494	39.30187843499219	23.858054554048003	13.524682452680315
7	73.1250912492298	5.757053101483991	16.63804430938871	4.479811339897504
8	72.57524504284693	11.746590681319335	9.904036742198345	5.774127533635394
9	67.50648952149523	6.5667276232143	10.908582500439232	15.018200354851242
10	42.44035702885084	23.430946294724496	19.362034876383586	14.766661800041078
11	35.50603420330452	22.634263188761558	23.07844588023647	18.78125672769745
12	28.70348145197012	20.358538329625624	27.641649736583577	23.29633048182068
13	25.318784597377466	24.17949929846355	32.8620955029905	17.639620601168485
14	20.35321803554946	31.25648024191253	26.722352411330498	21.66794931120751
15	17.698886202621544	24.2945661238317	37.28301242474259	20.72353524880417
16	19.401875218070195	24.82028541521802	29.717554248440408	26.060285118271377
17	19.87501020754096	25.853659743685554	30.104203527231242	24.167126521542244
18	20.672188224580747	25.18231286793545	29.993714629323975	24.15178427815982
19	21.842776649105573	26.814282143855806	27.10454749042966	24.238393716608968
20	24.961087616582493	25.279067983460074	30.917094970961088	18.842749428996346
21	22.47861365509152	29.678085090061444	28.034609131604277	19.808692123242757
22	20.617748006126998	23.52794886578754	29.99198244055499	25.862320687530467
23	21.32856404025607	28.716225412199066	28.108350882055266	21.846859665489603
24	22.947913083716685	24.450586840809375	27.932409994234288	24.669090081239652
25	19.088472778653497	31.25672769745096	27.00977201921245	22.645027504683096
26	18.26840512430929	27.71007119295841	30.060775080237455	23.960748602494846
27	22.83037170296427	28.906889904556397	26.90534578199662	21.357392610482712
28	18.724094498321016	27.362272433700475	31.831319457676443	22.08231361030207
29	21.335740250870426	25.844503888763786	28.446746330853006	24.373009529512785
30	19.657620517033603	31.288402006369502	27.073120637049534	21.980856839547354
31	24.550558878333536	26.70490679587145	23.063474820161687	25.681059505633325
32	23.780353514982195	30.043205737009202	22.403634626948403	23.7728061210602
33	21.379787336710276	27.39864839784912	22.61248710138006	28.609077164060547
34	21.552016391454863	30.07054957400529	23.90692702288716	24.470507011652682
35	20.280342379482967	27.46410038776283	23.812027823900742	28.443529408853465
36	22.649357976605554	32.478415690660775	22.354143519263175	22.51808281347049
37	19.822797088933044	29.894113775107456	27.13411842727158	23.148970708687916
38	21.979990745162866	26.595407720117887	22.267410353044816	29.15719118167443
39	21.702593086587168	28.30532549064247	24.75941135276519	25.23267007000517
40	19.954195979837323	25.08939331325644	26.422065115450383	28.534345591455857
41	18.398937920829077	31.749040491149756	23.973863746031434	25.87815784198974
42	19.737548655945243	25.78486710400309	27.836026062017304	26.641558178034362
43	22.811688809813095	27.654022513504884	24.639147961090092	24.895140715591925
44	21.609549804138943	25.952270775748364	27.237554842333704	25.200624577778985
45	20.13533343396525	24.488323810419363	26.00188561120281	29.37445714441258
46	24.794921222529343	27.32738120278239	24.371895979589866	23.5058015950984
47	18.61335814487532	26.09690853795844	31.84282614021326	23.44690717695298
48	20.799009188024144	27.05542756605207	25.666830812173824	26.47873243374997
49	19.470049218906592	22.848188501730952	32.27191404384418	25.409848235518282
50	20.72526743757315	24.32525061059654	27.93377099969563	27.015710952134675
51	20.217241217184302	24.73553189330707	24.48374588295848	30.56348100655015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	189.0
1	412.0
2	635.0
3	2865.0
4	5095.0
5	3525.0
6	1955.0
7	1938.5
8	1922.0
9	2010.0
10	2098.0
11	2031.5
12	1965.0
13	1929.0
14	1893.0
15	1912.5
16	1932.0
17	1968.5
18	2005.0
19	2131.5
20	2258.0
21	2244.0
22	2230.0
23	2646.5
24	3063.0
25	3741.5
26	4943.0
27	5466.0
28	6731.5
29	7997.0
30	9248.5
31	10500.0
32	11502.5
33	12505.0
34	14696.5
35	16888.0
36	18537.0
37	20186.0
38	21795.5
39	23405.0
40	27856.0
41	32307.0
42	37146.5
43	41986.0
44	49167.0
45	56348.0
46	87072.5
47	117797.0
48	100222.5
49	82648.0
50	80953.5
51	79259.0
52	68414.0
53	57569.0
54	51978.5
55	46388.0
56	42271.5
57	38155.0
58	35925.5
59	33696.0
60	30529.0
61	27362.0
62	24433.0
63	21504.0
64	19407.0
65	17310.0
66	13396.0
67	9482.0
68	7956.0
69	6430.0
70	5433.5
71	4437.0
72	3619.5
73	2802.0
74	2193.5
75	1387.5
76	1190.0
77	1102.5
78	1015.0
79	599.0
80	183.0
81	147.5
82	112.0
83	65.5
84	19.0
85	13.0
86	7.0
87	16.0
88	25.0
89	13.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	808226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.455099002887668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4504244477474	16.758767792202605
2	11.62301609325505	5.452379863589076
3	4.827569090634955	3.396933328923698
4	2.4906641504336573	2.336750969254581
5	1.4701582959137243	1.7241354190286513
6	0.941351325192701	1.3247693117336583
7	0.6729006150383063	1.1048065381379233
8	0.475799880812972	0.8927946648024327
9	0.3710789765730954	0.7833324720070968
>10	3.775351936685477	21.633011416987117
>50	1.6411511137082626	27.419820920922728
>100	0.24825480412306505	7.865719034780145
>500	0.005872694291083259	0.8617804692800485
>1k	0.004271050393515098	2.41353629248818
>5k	5.338812991893873E-4	0.6276146050612618
>10k+	0.0016016438975681618	5.403846900800816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGC	16917	2.0931026717774484	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCG	14268	1.7653478111320349	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTC	11969	1.4808976697111946	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	5012	0.62012357929589	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCCACAT	3711	0.4591537515496903	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2913	0.3604189917176631	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCT	2791	0.345324203873669	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	2542	0.31451598933961544	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTA	2189	0.27084008680740285	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTC	1774	0.21949306258398021	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	1756	0.21726596273814502	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	1598	0.1977169752024805	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	910	0.11259226998389063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4745553842613326E-4	0.0	0.0	0.2307522895823693	0.0
2	2.4745553842613326E-4	0.0	0.0	1.4325201119488855	0.0
3	2.4745553842613326E-4	0.0	0.0	1.9059025569580785	0.0
4	2.4745553842613326E-4	0.0	0.0	2.58096126578457	0.0
5	2.4745553842613326E-4	0.0	0.0	5.414698364071436	0.0
6	2.4745553842613326E-4	0.0	0.0	6.084560506590979	0.0
7	2.4745553842613326E-4	0.0	0.0	7.106428152521696	0.0
8	2.4745553842613326E-4	0.0	0.0	8.32551786257804	0.0
9	2.4745553842613326E-4	0.0	0.0	8.66564550014476	0.0
10	2.4745553842613326E-4	0.0	0.0	11.374912462603282	0.0
11	2.4745553842613326E-4	0.0	0.0	12.691252199261097	0.0
12	2.4745553842613326E-4	0.0	0.0	15.989958254250668	0.0
13	2.4745553842613326E-4	0.0	0.0	16.50689287402286	0.0
14	2.4745553842613326E-4	0.0	0.0	16.74123326891241	0.0
15	2.4745553842613326E-4	0.0	0.0	17.36754323666895	0.0
16	2.4745553842613326E-4	0.0	0.0	18.126241917483476	0.0
17	2.4745553842613326E-4	0.0	0.0	18.94952649382722	0.0
18	2.4745553842613326E-4	0.0	0.0	19.778997458631622	0.0
19	2.4745553842613326E-4	0.0	0.0	20.850356212247565	0.0
20	2.4745553842613326E-4	0.0	0.0	21.45328163162284	0.0
21	2.4745553842613326E-4	0.0	0.0	22.088128815455082	0.0
22	2.4745553842613326E-4	0.0	0.0	22.813668454120506	0.0
23	2.4745553842613326E-4	0.0	0.0	23.5161947277123	0.0
24	2.4745553842613326E-4	0.0	0.0	24.076062883401423	0.0
25	2.4745553842613326E-4	0.0	0.0	24.55798254448632	0.0
26	3.7118330763919995E-4	0.0	0.0	25.01354819072883	0.0
27	3.7118330763919995E-4	0.0	0.0	25.50202542358202	0.0
28	4.949110768522665E-4	0.0	0.0	25.95759106982453	0.0
29	6.186388460653332E-4	0.0	0.0	26.391628084223967	0.0
30	6.186388460653332E-4	0.0	0.0	26.88443578899961	0.0
31	6.186388460653332E-4	0.0	0.0	27.40322632531	0.0
32	6.186388460653332E-4	0.0	0.0	27.854832682937694	0.0
33	6.186388460653332E-4	0.0	0.0	28.31707962871771	0.0
34	6.186388460653332E-4	0.0	0.0	28.765221608807437	0.0
35	6.186388460653332E-4	0.0	0.0	29.247264997661546	0.0
36	6.186388460653332E-4	0.0	0.0	29.715203420825365	0.0
37	6.186388460653332E-4	0.0	0.0	30.185987582681083	0.0
38	6.186388460653332E-4	0.0	0.0	30.62089069146501	0.0
39	6.186388460653332E-4	0.0	0.0	31.045648123173468	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTCC	30	2.1646993E-6	45.000004	19
AATGCGA	30	2.1646993E-6	45.000004	25
CCGTAAC	30	2.1646993E-6	45.000004	40
CGAATAT	30	2.1646993E-6	45.000004	14
GAGCCGT	30	2.1646993E-6	45.000004	31
TTACGTA	30	2.1646993E-6	45.000004	18
CTCGACG	30	2.1646993E-6	45.000004	1
ATGCGAA	30	2.1646993E-6	45.000004	26
CGGGTTA	40	6.8102963E-9	45.0	6
CTTCGAA	20	7.032042E-4	45.0	31
AGCCGTA	25	3.8897742E-5	45.0	32
GGTACGA	20	7.032042E-4	45.0	8
GCGACTA	25	3.8897742E-5	45.0	28
CTCGTCG	20	7.032042E-4	45.0	1
ATTAGCG	45	3.8380676E-10	45.0	1
CACGCAA	25	3.8897742E-5	45.0	41
CGTCTCA	20	7.032042E-4	45.0	40
ATCGTCC	20	7.032042E-4	45.0	45
AAACGCG	20	7.032042E-4	45.0	1
TACGAGC	25	3.8897742E-5	45.0	28
>>END_MODULE