SRR3549853_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1157624
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	3227	0.27876063385002386	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2835	0.24489817073592118	No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2797	0.24161558502588057	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	1954	0.16879401256366489	TruSeq Adapter, Index 15 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	20	7.033306E-4	45.000004	6
CTCGTCT	40	6.8157533E-9	45.000004	17
GTCGACG	20	7.033306E-4	45.000004	2
CGGTGAT	20	7.033306E-4	45.000004	45
CATACGT	20	7.033306E-4	45.000004	44
ATACGTC	20	7.033306E-4	45.000004	45
ATACGTA	20	7.033306E-4	45.000004	43
CGCCGTA	20	7.033306E-4	45.000004	20
GCGTATA	20	7.033306E-4	45.000004	7
TACGTAA	20	7.033306E-4	45.000004	44
TCGTAAG	55	1.8189894E-12	45.0	1
GTATACG	45	3.8562575E-10	45.0	1
TCGCGCA	35	1.211938E-7	45.0	34
CGAACTA	50	2.1827873E-11	44.999996	36
CGACACG	25	3.890824E-5	44.999996	33
CGTTAGT	25	3.890824E-5	44.999996	23
CCGAACT	55	6.184564E-11	40.909092	35
ATCGCAA	55	6.184564E-11	40.909092	39
CCCGAAC	55	6.184564E-11	40.909092	34
CGAGGGT	105	0.0	40.714287	4