FastQCFastQC Report
Sat 18 Jun 2016
SRR3549852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364117
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC114850.8419365787538753No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG104740.7678227014251711No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC91650.671863190620746No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC64110.46997434970753976No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC52390.38405796570235545No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41180.30188026393630457No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT32670.23949558578919553No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGACAAT27060.1983700811587276No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT21090.15460550671240078No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC18860.13825793535305256No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTA15820.11597245690802183No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTC15740.11538599694894207No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT13850.10153088041568283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA302.1658016E-645.00000433
CGCTATA302.1658016E-645.00000412
ATTACGC207.0337515E-445.025
ATATGCG207.0337515E-445.01
TGCGATC207.0337515E-445.042
CTCGACG207.0337515E-445.01
ACGTATC207.0337515E-445.036
ATACGAG207.0337515E-445.01
CGTCAAT207.0337515E-445.014
ATCGATT207.0337515E-445.014
CTACGCG253.8911916E-544.9999961
TATTACG253.8911916E-544.9999961
CCGAACG253.8911916E-544.99999637
CGTTTTT23950.042.557411
TCGTGCG501.0822987E-940.4999961
TGCGTAG900.040.01
TAATACG403.458863E-739.3751
GATACGA403.458863E-739.37511
GGATCGA800.039.3758
GCCCGAT403.458863E-739.37533