##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1364117
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23278721693227	33.0	31.0	34.0	30.0	34.0
2	32.40870614470753	34.0	31.0	34.0	30.0	34.0
3	32.42386833387459	34.0	31.0	34.0	30.0	34.0
4	35.96530356267095	37.0	35.0	37.0	35.0	37.0
5	35.85125249520386	37.0	35.0	37.0	35.0	37.0
6	35.770669231451556	37.0	35.0	37.0	35.0	37.0
7	36.053839223468366	37.0	35.0	37.0	35.0	37.0
8	35.78651831184568	37.0	35.0	37.0	35.0	37.0
9	37.5368842995139	39.0	38.0	39.0	35.0	39.0
10	37.32083098443902	39.0	37.0	39.0	34.0	39.0
11	37.219405666815966	39.0	37.0	39.0	34.0	39.0
12	37.12369540149415	39.0	37.0	39.0	34.0	39.0
13	37.05609269586113	39.0	37.0	39.0	33.0	39.0
14	38.229855650211825	40.0	37.0	41.0	33.0	41.0
15	38.267342903871146	40.0	37.0	41.0	34.0	41.0
16	38.39623947212739	40.0	38.0	41.0	34.0	41.0
17	38.329141855134125	40.0	37.0	41.0	34.0	41.0
18	38.31232878118226	40.0	37.0	41.0	34.0	41.0
19	38.309821664857196	40.0	37.0	41.0	34.0	41.0
20	38.17582142880706	40.0	37.0	41.0	34.0	41.0
21	38.175009181763734	40.0	37.0	41.0	34.0	41.0
22	38.118835847658225	40.0	37.0	41.0	34.0	41.0
23	37.93699147507142	40.0	36.0	41.0	33.0	41.0
24	37.697178467829374	40.0	36.0	41.0	33.0	41.0
25	37.6639239889247	40.0	36.0	41.0	33.0	41.0
26	37.79485484016401	40.0	36.0	41.0	33.0	41.0
27	37.79356682747888	40.0	36.0	41.0	33.0	41.0
28	37.77707117497986	40.0	36.0	41.0	33.0	41.0
29	37.79435854842363	40.0	36.0	41.0	33.0	41.0
30	37.610446904481066	40.0	36.0	41.0	33.0	41.0
31	37.58112977112667	40.0	36.0	41.0	33.0	41.0
32	37.440539191286376	40.0	36.0	41.0	33.0	41.0
33	37.25345773126499	40.0	35.0	41.0	32.0	41.0
34	37.24630218668926	40.0	36.0	41.0	32.0	41.0
35	37.11838867193943	40.0	35.0	41.0	32.0	41.0
36	37.05877061864928	39.0	35.0	41.0	31.0	41.0
37	36.98542133849222	39.0	35.0	41.0	31.0	41.0
38	36.910152868119084	39.0	35.0	41.0	31.0	41.0
39	36.961185147608305	39.0	35.0	41.0	31.0	41.0
40	36.83817663734123	39.0	35.0	41.0	31.0	41.0
41	36.78509028184533	39.0	35.0	41.0	31.0	41.0
42	36.70599882561393	39.0	35.0	41.0	31.0	41.0
43	36.69116652017386	39.0	35.0	41.0	31.0	41.0
44	36.65761074746521	39.0	35.0	41.0	31.0	41.0
45	36.59964944355946	39.0	35.0	41.0	31.0	41.0
46	36.53427381962104	39.0	35.0	41.0	31.0	41.0
47	36.3529587271473	39.0	35.0	41.0	30.0	41.0
48	36.32806790033406	39.0	35.0	40.0	31.0	41.0
49	36.30951157415383	39.0	35.0	40.0	31.0	41.0
50	36.21845413553236	39.0	35.0	40.0	30.0	41.0
51	35.276993102497805	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	15.0
10	24.0
11	26.0
12	18.0
13	17.0
14	20.0
15	38.0
16	79.0
17	136.0
18	302.0
19	585.0
20	1146.0
21	2106.0
22	3208.0
23	4235.0
24	5590.0
25	7604.0
26	9871.0
27	11917.0
28	13495.0
29	15618.0
30	19076.0
31	24881.0
32	32217.0
33	44755.0
34	81224.0
35	106197.0
36	99712.0
37	157073.0
38	286028.0
39	436591.0
40	301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61839930152619	21.154856951419855	23.264866576693937	11.96187717036002
2	29.572096821606948	27.793876918182235	24.730283399444474	17.903742860766343
3	30.09895778734522	27.2902544283225	26.288727433204045	16.32206035112824
4	27.83742157014391	24.288019282803454	29.681911448944632	18.192647698108008
5	28.2193536184946	28.327262250965273	24.291244812578395	19.162139317961728
6	25.032676815844972	35.66336318658883	23.876397699024352	15.427562298541842
7	78.2883726249288	5.357678263667999	11.494688505458111	4.859260605945091
8	78.64457374257486	7.470400266252822	8.544941526276704	5.3400844648956065
9	73.86778406837536	6.132025332137933	10.108883622152645	9.891306977334056
10	39.9989150490757	25.938170992664116	18.486024292637655	15.576889665622524
11	29.999626131776086	25.84287124931366	24.50244370534199	19.65505891356826
12	28.07244539874512	22.04121787207402	28.933955078633282	20.95238165054757
13	24.700447249026293	24.466229802868817	30.14953995881585	20.683782989289043
14	19.266235960698385	29.159082395425024	28.458849204283798	23.115832439592793
15	18.482798762862714	26.103112856155303	34.7695249014564	20.644563479525583
16	20.26219158620558	25.754022565513075	30.068828406947496	23.914957441333843
17	19.926516567127305	26.292466115443176	29.24470554945067	24.536311767978848
18	21.043869404164013	26.614799170452386	29.508465916046788	22.83286550933681
19	22.61191671975351	27.80362681500194	26.54735627515822	23.037100190086335
20	24.491300966119475	26.8371408024385	29.53023824202763	19.1413199894144
21	23.678320847845164	27.994152994207976	27.869310330418873	20.45821582752799
22	21.499842022348524	25.292038732747997	28.049060307876815	25.159058937026664
23	21.645577322179843	27.27420008694269	28.048693770402394	23.031528820475078
24	22.696660183840535	24.430529052859836	28.66946163708831	24.203349126211315
25	20.925551107419672	28.617120085740446	25.997550063520947	24.45977874331894
26	21.04724154892872	27.753337873510848	27.828551363262825	23.370869214297603
27	22.82670767976647	27.691466347827937	27.57256159112452	21.909264381281076
28	19.882385455206556	27.1678309118646	30.03642649420834	22.913357138720507
29	25.09454834152789	24.049110156973338	28.281958219126363	22.574383282372406
30	22.53860922486854	27.075756698289076	28.99304091951057	21.392593157331813
31	25.234785579242835	25.8924271158559	24.838851799369117	24.03393550553215
32	26.28117676123089	28.69717187015483	23.960554703152294	21.06109666546198
33	22.79833767924599	28.938500143316155	25.373116822090775	22.890045355347084
34	23.994935918253347	26.371711517413825	26.07217709331384	23.56117547101898
35	22.199195523551133	26.938378452874645	27.342595979670364	23.51983004390386
36	23.538303532614872	30.038112566590698	25.073142553021476	21.350441347772957
37	23.1699333708179	27.924804104046792	28.29354080331819	20.611721721817116
38	22.432753202254645	28.93637422596449	25.133694543796466	23.497178027984404
39	24.571719288008286	26.768524987226165	25.950706574289452	22.709049150476098
40	22.19553014880688	26.482845679659444	28.462294656543392	22.859329514990286
41	20.007887886449623	28.515076052860564	26.374863739693883	25.102172320995926
42	21.99943259998959	27.896360796031427	27.28321690881354	22.820989695165444
43	24.46366404054784	26.632612891709435	26.515394207388372	22.388328860354353
44	23.08233091443036	25.610559798023193	27.158520860014207	24.148588427532243
45	22.76930791127154	24.48609613398264	26.144604898260194	26.59999105648562
46	24.269472486597557	26.74491997387321	26.049451769899502	22.936155769629732
47	21.00311043700797	26.127817481931537	30.532791542074474	22.336280538986024
48	21.70972138020419	25.186109402639218	27.767852757498073	25.33631645965852
49	21.843287635884607	24.20474196861413	30.76656914326264	23.185401252238627
50	21.371993751269137	24.10328439569333	29.265891415472428	25.258830437565106
51	20.45367076284512	23.504288854988246	26.736709534446092	29.30533084772054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	287.0
1	808.0
2	1329.0
3	4474.5
4	7620.0
5	5234.5
6	2849.0
7	2751.0
8	2653.0
9	2610.5
10	2568.0
11	2596.0
12	2624.0
13	2477.5
14	2331.0
15	2275.5
16	2220.0
17	2287.0
18	2354.0
19	2486.5
20	2619.0
21	2875.0
22	3131.0
23	3783.5
24	4436.0
25	5695.5
26	8682.0
27	10409.0
28	12543.0
29	14677.0
30	17065.0
31	19453.0
32	22880.0
33	26307.0
34	29765.5
35	33224.0
36	36210.5
37	39197.0
38	44342.5
39	49488.0
40	54449.0
41	59410.0
42	64758.5
43	70107.0
44	77694.0
45	85281.0
46	121357.5
47	157434.0
48	150887.5
49	144341.0
50	143136.0
51	141931.0
52	121200.0
53	100469.0
54	90215.5
55	79962.0
56	72531.0
57	65100.0
58	61719.0
59	58338.0
60	55530.0
61	52722.0
62	46978.5
63	41235.0
64	34093.0
65	26951.0
66	22053.0
67	17155.0
68	14357.0
69	11559.0
70	8826.0
71	6093.0
72	5206.0
73	4319.0
74	3252.0
75	1843.0
76	1501.0
77	1132.5
78	764.0
79	460.5
80	157.0
81	177.0
82	197.0
83	172.0
84	147.0
85	81.0
86	15.0
87	11.5
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1364117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.559294870854693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39560515696685	16.584708195052414
2	12.009907895815648	5.658899229786537
3	4.948358467723213	3.497395088033456
4	2.5817732150099326	2.4329902584837417
5	1.5030218310635586	1.7705067257679163
6	1.0340238134190438	1.4616523154294934
7	0.7040593101824976	1.1610998626612006
8	0.5190856402884745	0.9783433330226065
9	0.3952926868657236	0.8381535273145813
>10	3.89188378939134	22.304993277182703
>50	1.7511156562877601	29.403098510307785
>100	0.2564444328377277	8.369166062046117
>500	0.004399781935823773	0.714410305841137
>1k	0.0034569715210043934	1.6576036819537128
>5k	9.428104148193799E-4	1.5411390316181226
>10k+	6.285402765462533E-4	1.6258405954985518
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	11485	0.8419365787538753	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	10474	0.7678227014251711	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	9165	0.671863190620746	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	6411	0.46997434970753976	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	5239	0.38405796570235545	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4118	0.30188026393630457	No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	3267	0.23949558578919553	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGACAAT	2706	0.1983700811587276	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT	2109	0.15460550671240078	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1886	0.13825793535305256	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTA	1582	0.11597245690802183	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTC	1574	0.11538599694894207	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT	1385	0.10153088041568283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1992248465490863E-4	0.0	0.0	0.25474354472526917	0.0
2	2.1992248465490863E-4	0.0	0.0	1.417693643580426	0.0
3	2.1992248465490863E-4	0.0	0.0	1.9578232658928816	0.0
4	2.1992248465490863E-4	0.0	0.0	2.5818166623537424	0.0
5	2.1992248465490863E-4	0.0	0.0	4.749592593597177	0.0
6	2.1992248465490863E-4	0.0	0.0	5.531783564019801	0.0
7	2.1992248465490863E-4	0.0	0.0	6.512051385621614	0.0
8	2.1992248465490863E-4	0.0	0.0	7.813186112334939	0.0
9	2.1992248465490863E-4	0.0	0.0	8.191452785941381	0.0
10	2.1992248465490863E-4	0.0	0.0	9.97590382643131	0.0
11	2.1992248465490863E-4	0.0	0.0	11.442860106574436	0.0
12	2.1992248465490863E-4	0.0	0.0	13.488652366329282	0.0
13	2.1992248465490863E-4	0.0	0.0	13.962658628255495	0.0
14	2.1992248465490863E-4	0.0	0.0	14.172977831080471	0.0
15	2.1992248465490863E-4	0.0	0.0	14.645957788078295	0.0
16	2.1992248465490863E-4	0.0	0.0	15.415686484370475	0.0
17	2.1992248465490863E-4	0.0	0.0	16.372642522598866	0.0
18	2.1992248465490863E-4	0.0	0.0	17.313177681973027	0.0
19	2.1992248465490863E-4	0.0	0.0	18.14499782643278	0.0
20	2.1992248465490863E-4	0.0	0.0	18.790910163864243	0.0
21	2.1992248465490863E-4	0.0	0.0	19.516800978215212	0.0
22	2.1992248465490863E-4	0.0	0.0	20.370540063645567	0.0
23	2.1992248465490863E-4	0.0	0.0	21.14562020706435	0.0
24	2.1992248465490863E-4	0.0	0.0	21.795930994188915	0.0
25	2.1992248465490863E-4	0.0	0.0	22.351455190427213	0.0
26	2.1992248465490863E-4	0.0	0.0	22.859329514990282	0.0
27	2.1992248465490863E-4	0.0	0.0	23.38193864602523	0.0
28	2.1992248465490863E-4	0.0	0.0	23.892085502929735	0.0
29	2.1992248465490863E-4	0.0	0.0	24.423271610866223	0.0
30	2.1992248465490863E-4	0.0	0.0	25.030477590998427	0.0
31	2.1992248465490863E-4	0.0	0.0	25.605135043401702	0.0
32	2.1992248465490863E-4	0.0	0.0	26.13881360616428	0.0
33	2.1992248465490863E-4	0.0	0.0	26.633932426617363	0.0
34	2.1992248465490863E-4	0.0	0.0	27.152436337938756	0.0
35	2.1992248465490863E-4	0.0	0.0	27.699750094749938	0.0
36	2.1992248465490863E-4	0.0	0.0	28.251462301254218	0.0
37	2.1992248465490863E-4	0.0	0.0	28.78829308629685	0.0
38	2.1992248465490863E-4	0.0	0.0	29.333847463230793	0.0
39	2.1992248465490863E-4	0.0	0.0	29.85674982424528	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	30	2.1658016E-6	45.000004	33
CGCTATA	30	2.1658016E-6	45.000004	12
ATTACGC	20	7.0337515E-4	45.0	25
ATATGCG	20	7.0337515E-4	45.0	1
TGCGATC	20	7.0337515E-4	45.0	42
CTCGACG	20	7.0337515E-4	45.0	1
ACGTATC	20	7.0337515E-4	45.0	36
ATACGAG	20	7.0337515E-4	45.0	1
CGTCAAT	20	7.0337515E-4	45.0	14
ATCGATT	20	7.0337515E-4	45.0	14
CTACGCG	25	3.8911916E-5	44.999996	1
TATTACG	25	3.8911916E-5	44.999996	1
CCGAACG	25	3.8911916E-5	44.999996	37
CGTTTTT	2395	0.0	42.55741	1
TCGTGCG	50	1.0822987E-9	40.499996	1
TGCGTAG	90	0.0	40.0	1
TAATACG	40	3.458863E-7	39.375	1
GATACGA	40	3.458863E-7	39.375	11
GGATCGA	80	0.0	39.375	8
GCCCGAT	40	3.458863E-7	39.375	33
>>END_MODULE