SRR3549851_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	759180
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	4200	0.5532284833636292	Illumina PCR Primer Index 11 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	3350	0.441265576016228	RNA PCR Primer, Index 11 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2958	0.38963091756895596	No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	2732	0.35986195632129403	TruSeq Adapter, Index 11 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC	2328	0.30664664506441164	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG	1976	0.26028082931584073	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	1879	0.24750388577149027	RNA PCR Primer, Index 11 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1420	0.18704391580389368	Illumina PCR Primer Index 11 (100% over 24bp)
ACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	987	0.13000869359045286	TruSeq Adapter, Index 11 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	962	0.12671566690376457	TruSeq Adapter, Index 11 (96% over 25bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	812	0.10695750678363497	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	779	0.10261071155720645	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGG	65	0.0	45.000004	2
GCCCCGT	20	7.031772E-4	45.0	29
TGTTTCG	20	7.031772E-4	45.0	19
TCGCATA	25	3.88955E-5	45.0	26
CGAACCG	20	7.031772E-4	45.0	36
GTCGATA	20	7.031772E-4	45.0	38
CGGTTCA	20	7.031772E-4	45.0	10
GACGTAG	50	2.1827873E-11	45.0	1
GACGTAC	35	1.2111923E-7	45.0	9
AAGCGTC	20	7.031772E-4	45.0	23
TACGGAT	20	7.031772E-4	45.0	24
TAACGCA	25	3.88955E-5	45.0	22
CCTAACG	20	7.031772E-4	45.0	40
CACTCGA	25	3.88955E-5	45.0	44
TCGTGCG	20	7.031772E-4	45.0	1
GCTTCGT	20	7.031772E-4	45.0	34
CTACGCG	20	7.031772E-4	45.0	1
CGCTAAT	20	7.031772E-4	45.0	45
TCTAAGT	20	7.031772E-4	45.0	12
AACGCAT	25	3.88955E-5	45.0	23