##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	759180
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19997629020786	33.0	31.0	34.0	30.0	34.0
2	32.38333728496536	34.0	31.0	34.0	30.0	34.0
3	32.379048447008614	34.0	31.0	34.0	30.0	34.0
4	35.91916541531653	37.0	35.0	37.0	35.0	37.0
5	35.814271977660106	37.0	35.0	37.0	35.0	37.0
6	35.7311349087173	37.0	35.0	37.0	35.0	37.0
7	36.02954503543297	37.0	35.0	37.0	35.0	37.0
8	35.79120234990384	37.0	35.0	37.0	35.0	37.0
9	37.46071814325983	39.0	37.0	39.0	35.0	39.0
10	37.321695777022576	39.0	37.0	39.0	34.0	39.0
11	37.1904554914513	39.0	37.0	39.0	34.0	39.0
12	36.96962907347401	39.0	35.0	39.0	33.0	39.0
13	36.83175004610237	39.0	35.0	39.0	33.0	39.0
14	37.88114281198135	40.0	36.0	41.0	33.0	41.0
15	37.96191417055244	40.0	36.0	41.0	33.0	41.0
16	38.09969440712347	40.0	36.0	41.0	33.0	41.0
17	38.073420005795725	40.0	36.0	41.0	33.0	41.0
18	38.052424984852074	40.0	36.0	41.0	34.0	41.0
19	38.01777970968677	40.0	36.0	41.0	34.0	41.0
20	37.94264996443531	40.0	36.0	41.0	34.0	41.0
21	37.885471166258334	40.0	36.0	41.0	33.0	41.0
22	37.83339392502437	40.0	35.0	41.0	33.0	41.0
23	37.662684738797125	39.0	35.0	41.0	33.0	41.0
24	37.42450275297031	39.0	35.0	41.0	33.0	41.0
25	37.35779130114071	39.0	35.0	41.0	33.0	41.0
26	37.517431966068656	39.0	35.0	41.0	33.0	41.0
27	37.54307805790458	39.0	35.0	41.0	33.0	41.0
28	37.438698332411285	39.0	35.0	41.0	33.0	41.0
29	37.449054242735585	39.0	35.0	41.0	33.0	41.0
30	37.32596222239785	39.0	35.0	41.0	33.0	41.0
31	37.15762269817434	39.0	35.0	41.0	32.0	41.0
32	37.0775665849996	39.0	35.0	41.0	32.0	41.0
33	36.96546405332069	39.0	35.0	41.0	32.0	41.0
34	36.83928317395084	39.0	35.0	41.0	31.0	41.0
35	36.80530967622962	39.0	35.0	41.0	31.0	41.0
36	36.74391053505098	39.0	35.0	41.0	31.0	41.0
37	36.62555125266735	39.0	35.0	41.0	31.0	41.0
38	36.55473932400748	39.0	35.0	41.0	31.0	41.0
39	36.590618825574964	39.0	35.0	41.0	31.0	41.0
40	36.5374285413209	39.0	35.0	41.0	31.0	41.0
41	36.45967491240549	39.0	35.0	41.0	31.0	41.0
42	36.3776811823283	39.0	35.0	41.0	31.0	41.0
43	36.3116823414737	39.0	35.0	41.0	31.0	41.0
44	36.23658025764641	39.0	35.0	41.0	30.0	41.0
45	36.17950025027003	38.0	35.0	40.0	30.0	41.0
46	36.115716957770225	38.0	35.0	40.0	30.0	41.0
47	35.97235174793857	38.0	35.0	40.0	30.0	41.0
48	35.95166890592481	38.0	35.0	40.0	30.0	41.0
49	35.92940936273347	38.0	35.0	40.0	30.0	41.0
50	35.833354408704125	38.0	35.0	40.0	30.0	41.0
51	34.86555889248926	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	9.0
11	26.0
12	19.0
13	17.0
14	23.0
15	36.0
16	69.0
17	111.0
18	192.0
19	340.0
20	625.0
21	1041.0
22	1638.0
23	2340.0
24	3353.0
25	4527.0
26	6320.0
27	7716.0
28	8613.0
29	9794.0
30	11714.0
31	15016.0
32	19692.0
33	28528.0
34	54818.0
35	69834.0
36	56943.0
37	87556.0
38	153717.0
39	214384.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97881925235122	21.90323770383835	24.24721409942306	12.870728944387366
2	34.10917042071709	24.63039068468611	23.545009088753655	17.715429805843147
3	32.22397850312179	23.76037303406307	26.745304143944782	17.27034431887036
4	29.202824099686502	24.44558602702916	27.871914433994572	18.47967543928976
5	26.257541031112513	29.797808161437338	24.33204246687215	19.612608340577992
6	26.310097737032063	33.870755288600854	24.209805316262283	15.609341658104798
7	77.73861271371743	6.213809636713296	10.761742933164731	5.285834716404541
8	76.87926446955926	6.523617587396928	10.138438841908375	6.458679101135435
9	70.92112542480045	7.485839985247241	12.461076424563345	9.131958165388971
10	42.06262019547407	24.68189362206591	19.349034484575466	13.90645169788456
11	32.519824020653864	23.519455201664954	24.758160120129613	19.20256065755157
12	30.144102847809478	20.605126583945836	29.46244632366501	19.788324244579677
13	25.800205484865252	24.376827629811114	29.911351721594354	19.911615163729287
14	19.570984483258254	27.53220580099581	29.69809531336442	23.19871440238152
15	18.65460101688664	25.687715693247977	35.79572696857135	19.861956321294027
16	22.144814141573804	24.074527779973128	31.736347111356988	22.044310967096077
17	21.211965541768752	25.3456360810348	28.255749624594955	25.186648752601492
18	21.932084617613743	26.085381595932454	30.477225427434863	21.50530835901894
19	23.418688585052294	26.413103611791673	28.232303274585735	21.935904528570298
20	24.583102821465268	27.034431887036014	28.40538475723807	19.97708053426065
21	23.802260333517744	27.084222450538743	30.045838931478702	19.06767828446482
22	21.484759872494006	24.8831634131563	28.43950051371216	25.19257620063753
23	21.246081298242842	25.890697858215443	29.67399035801786	23.189230485523854
24	23.701361995837615	23.43423167101346	29.31939724439527	23.545009088753655
25	21.84027503358887	27.10095102610712	26.67075001975816	24.38802392054585
26	21.553518269712058	26.36463025896362	27.44210859084802	24.639742880476305
27	22.741642298269184	26.887562896809712	28.46650333254301	21.904291472378095
28	19.239705998577413	26.03954266445375	30.99844569140388	23.72230564556495
29	22.252430253694776	25.356700650702074	28.39972075133697	23.991148344266183
30	24.192154693221635	26.11093548302115	26.697752838589007	22.999156985168206
31	23.565294133143656	26.826707763639718	26.396770199425696	23.21122790379093
32	24.68531836982007	28.416449326905347	25.197054716931426	21.70117758634316
33	23.961642825153458	26.656524144471668	25.144893174214282	24.236939856160593
34	21.261097499934138	26.336178508390635	28.762612292210015	23.640111699465212
35	22.45804684001159	25.424800442582786	28.231249506045998	23.885903211359626
36	23.548170394372875	26.33736399799784	26.670486577623226	23.44397903000606
37	21.680629099818223	29.105218788693065	28.7602413129956	20.453910798493112
38	20.53821228167233	28.135093126794697	27.253088859032115	24.073605732500855
39	21.677599515266472	26.44498011011881	27.211465001712376	24.665955372902342
40	22.353855475644774	24.402776680102214	29.589952316973577	23.653415527279435
41	20.093258515767012	25.67730972891804	27.383887879027373	26.845543876287575
42	21.54706393740615	24.20427303142865	28.737980452593586	25.510682578571615
43	22.47964909507627	23.020364077030482	28.58953080955768	25.91045601833557
44	22.16641639663848	23.547775231170473	27.86243051713691	26.423377855054138
45	21.8649068732053	24.447693564108643	26.35053610474459	27.33686345794146
46	22.85465897415633	25.29057667483337	27.767591348560288	24.08717300245001
47	19.460865670855394	25.110513975605258	31.992808029716276	23.435812323823072
48	20.34892910772149	23.135488289997102	30.18928317395084	26.326299428330568
49	21.18430411760057	22.74348639321373	31.978318712294847	24.093890776890856
50	20.79704417924603	23.064754076767038	30.125398456229092	26.012803287757848
51	19.43030638320293	23.255881345662424	27.11306936431413	30.200742906820516
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	388.0
1	697.0
2	1006.0
3	3192.0
4	5378.0
5	3713.0
6	2048.0
7	1932.0
8	1816.0
9	1812.0
10	1808.0
11	1833.0
12	1858.0
13	1779.5
14	1701.0
15	1781.0
16	1861.0
17	1810.0
18	1759.0
19	1718.5
20	1678.0
21	1725.0
22	1772.0
23	2405.5
24	3039.0
25	3533.5
26	4999.5
27	5971.0
28	7077.0
29	8183.0
30	9293.0
31	10403.0
32	11827.0
33	13251.0
34	15113.5
35	16976.0
36	18667.5
37	20359.0
38	21847.0
39	23335.0
40	26301.5
41	29268.0
42	33411.0
43	37554.0
44	43328.5
45	49103.0
46	61073.5
47	73044.0
48	78846.5
49	84649.0
50	83056.5
51	81464.0
52	68656.0
53	55848.0
54	50041.5
55	44235.0
56	40964.0
57	37693.0
58	35900.0
59	34107.0
60	31988.5
61	29870.0
62	27408.0
63	24946.0
64	20654.0
65	16362.0
66	14142.0
67	11922.0
68	9972.5
69	8023.0
70	6405.5
71	4788.0
72	3799.5
73	2811.0
74	2345.5
75	1680.5
76	1481.0
77	1171.5
78	862.0
79	633.0
80	404.0
81	272.5
82	141.0
83	110.5
84	80.0
85	49.5
86	19.0
87	12.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	759180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.09268111790038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.57403950007318	17.457991872595414
2	12.360376074642081	6.20309950676638
3	5.175199705397742	3.8957890778699253
4	2.7217303907423522	2.7318205113518497
5	1.5635372729537835	1.9616671103090426
6	1.0039787972971503	1.5115511885826323
7	0.7280051626392792	1.2787320978804844
8	0.5242164484324561	1.052319694181911
9	0.4074158975242224	0.9200841479044644
>10	4.148786162318019	25.625272678996208
>50	1.6412400378539773	28.123211629919666
>100	0.14137624678402938	5.404083284121438
>500	0.005846386145204222	1.0546504432475095
>1k	0.004251917196512161	2.779726756273121
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	4200	0.5532284833636292	Illumina PCR Primer Index 11 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	3350	0.441265576016228	RNA PCR Primer, Index 11 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2958	0.38963091756895596	No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	2732	0.35986195632129403	TruSeq Adapter, Index 11 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC	2328	0.30664664506441164	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG	1976	0.26028082931584073	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	1879	0.24750388577149027	RNA PCR Primer, Index 11 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1420	0.18704391580389368	Illumina PCR Primer Index 11 (100% over 24bp)
ACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	987	0.13000869359045286	TruSeq Adapter, Index 11 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	962	0.12671566690376457	TruSeq Adapter, Index 11 (96% over 25bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	812	0.10695750678363497	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	779	0.10261071155720645	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.634421349350615E-4	0.0	0.0	0.3842303538027872	0.0
2	2.634421349350615E-4	0.0	0.0	1.7825812060380937	0.0
3	2.634421349350615E-4	0.0	0.0	2.464632893384968	0.0
4	2.634421349350615E-4	0.0	0.0	3.2107010195210623	0.0
5	2.634421349350615E-4	0.0	0.0	5.2247161410996075	0.0
6	2.634421349350615E-4	0.0	0.0	6.151900735003556	0.0
7	2.634421349350615E-4	0.0	0.0	7.182486366869517	0.0
8	2.634421349350615E-4	0.0	0.0	8.64129718907242	0.0
9	2.634421349350615E-4	0.0	0.0	9.139202824099687	0.0
10	2.634421349350615E-4	0.0	0.0	10.499881451039279	0.0
11	2.634421349350615E-4	0.0	0.0	12.287204615506203	0.0
12	2.634421349350615E-4	0.0	0.0	13.996944071234754	0.0
13	2.634421349350615E-4	0.0	0.0	14.555836560499486	0.0
14	2.634421349350615E-4	0.0	0.0	14.789773176321821	0.0
15	2.634421349350615E-4	0.0	0.0	15.293737980452594	0.0
16	2.634421349350615E-4	0.0	0.0	16.202086461708685	0.0
17	2.634421349350615E-4	0.0	0.0	17.241892568297374	0.0
18	2.634421349350615E-4	0.0	0.0	18.326879001027425	0.0
19	2.634421349350615E-4	0.0	0.0	19.08690956031508	0.0
20	2.634421349350615E-4	0.0	0.0	19.79504201902052	0.0
21	2.634421349350615E-4	0.0	0.0	20.60170183619168	0.0
22	2.634421349350615E-4	0.0	0.0	21.41481598566875	0.0
23	2.634421349350615E-4	0.0	0.0	22.223451618851918	0.0
24	2.634421349350615E-4	0.0	0.0	22.88548170394373	0.0
25	2.634421349350615E-4	0.0	0.0	23.468610869622488	0.0
26	2.634421349350615E-4	0.0	0.0	23.99971021365157	0.0
27	2.634421349350615E-4	0.0	0.0	24.542005848415396	0.0
28	2.634421349350615E-4	0.0	0.0	25.040174925577595	0.0
29	2.634421349350615E-4	0.0	0.0	25.57245975921389	0.0
30	2.634421349350615E-4	0.0	0.0	26.18469928080297	0.0
31	3.9516320240259227E-4	0.0	0.0	26.731209989725755	0.0
32	3.9516320240259227E-4	0.0	0.0	27.290892805395295	0.0
33	3.9516320240259227E-4	0.0	0.0	27.76969888563977	0.0
34	3.9516320240259227E-4	0.0	0.0	28.28117179061619	0.0
35	3.9516320240259227E-4	0.0	0.0	28.837166416396638	0.0
36	3.9516320240259227E-4	0.0	0.0	29.361021101715007	0.0
37	3.9516320240259227E-4	0.0	0.0	29.883558576358702	0.0
38	3.9516320240259227E-4	0.0	0.0	30.405700887799995	0.0
39	5.26884269870123E-4	0.0	0.0	31.03427382175505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGG	65	0.0	45.000004	2
GCCCCGT	20	7.031772E-4	45.0	29
TGTTTCG	20	7.031772E-4	45.0	19
TCGCATA	25	3.88955E-5	45.0	26
CGAACCG	20	7.031772E-4	45.0	36
GTCGATA	20	7.031772E-4	45.0	38
CGGTTCA	20	7.031772E-4	45.0	10
GACGTAG	50	2.1827873E-11	45.0	1
GACGTAC	35	1.2111923E-7	45.0	9
AAGCGTC	20	7.031772E-4	45.0	23
TACGGAT	20	7.031772E-4	45.0	24
TAACGCA	25	3.88955E-5	45.0	22
CCTAACG	20	7.031772E-4	45.0	40
CACTCGA	25	3.88955E-5	45.0	44
TCGTGCG	20	7.031772E-4	45.0	1
GCTTCGT	20	7.031772E-4	45.0	34
CTACGCG	20	7.031772E-4	45.0	1
CGCTAAT	20	7.031772E-4	45.0	45
TCTAAGT	20	7.031772E-4	45.0	12
AACGCAT	25	3.88955E-5	45.0	23
>>END_MODULE