FastQCFastQC Report
Sat 18 Jun 2016
SRR3549849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800059
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40790.2266036835459282No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC37330.20738209136478303No Hit
CCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC34890.19382698011565178No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT19980.11099636178591925No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC207.034351E-445.00000429
CGTACCC207.034351E-445.00000431
CGAACCG502.1827873E-1145.02
AAACGCG253.89169E-545.01
TCGAACG253.89169E-545.01
CTATCGT302.1661908E-644.9999961
TACGCTA302.1661908E-644.99999617
TTGCGAG1500.040.5000041
TGTCGCG451.929402E-840.01
GCGTAAG1850.038.918921
CGCCTAG356.2502586E-638.5714333
CGTAAGG3100.038.4677432
CGTTTTT25850.038.036751
ACGCGTA950.037.89473724
ACTATCG301.1401788E-437.4999961
ATATCGC301.1401788E-437.49999634
CGTAGTG301.1401788E-437.49999627
GTATACG601.5643309E-1037.4999961
CCGGTAT301.1401788E-437.49999642
ATAGACG1150.037.1739121