##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800059
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41509528298795	33.0	31.0	34.0	31.0	34.0
2	32.587536852958706	34.0	31.0	34.0	31.0	34.0
3	32.58763407199431	34.0	31.0	34.0	31.0	34.0
4	36.08483444153775	37.0	35.0	37.0	35.0	37.0
5	35.98565324803243	37.0	35.0	37.0	35.0	37.0
6	35.92086148287362	37.0	35.0	37.0	35.0	37.0
7	36.16483070832678	37.0	35.0	37.0	35.0	37.0
8	35.98041675300643	37.0	36.0	37.0	35.0	37.0
9	37.62799830450002	39.0	38.0	39.0	35.0	39.0
10	37.44792254031673	39.0	37.0	39.0	35.0	39.0
11	37.33928221241637	39.0	37.0	39.0	35.0	39.0
12	37.25781932703317	39.0	37.0	39.0	34.0	39.0
13	37.23095354096727	39.0	37.0	39.0	34.0	39.0
14	38.46336814515524	40.0	38.0	41.0	34.0	41.0
15	38.535755216912335	40.0	38.0	41.0	34.0	41.0
16	38.607435089627614	40.0	38.0	41.0	35.0	41.0
17	38.59093785259261	40.0	38.0	41.0	35.0	41.0
18	38.56462038188748	40.0	38.0	41.0	35.0	41.0
19	38.56692252865045	40.0	38.0	41.0	35.0	41.0
20	38.495044884639896	40.0	38.0	41.0	34.0	41.0
21	38.42978369042348	40.0	37.0	41.0	34.0	41.0
22	38.374192734793695	40.0	37.0	41.0	34.0	41.0
23	38.2312696417173	40.0	37.0	41.0	34.0	41.0
24	37.99862337845593	40.0	37.0	41.0	34.0	41.0
25	37.912948408913266	40.0	36.0	41.0	33.0	41.0
26	38.03544883806586	40.0	37.0	41.0	34.0	41.0
27	38.088887642016175	40.0	37.0	41.0	34.0	41.0
28	38.034646086600496	40.0	37.0	41.0	34.0	41.0
29	38.02575193368662	40.0	37.0	41.0	34.0	41.0
30	37.930149511766004	40.0	36.0	41.0	34.0	41.0
31	37.851169322783306	40.0	36.0	41.0	33.0	41.0
32	37.784900383820755	40.0	36.0	41.0	33.0	41.0
33	37.68493754926922	40.0	36.0	41.0	33.0	41.0
34	37.610007227540876	40.0	36.0	41.0	33.0	41.0
35	37.55181913481725	40.0	36.0	41.0	33.0	41.0
36	37.48160254747206	40.0	36.0	41.0	33.0	41.0
37	37.42717933134414	40.0	36.0	41.0	33.0	41.0
38	37.352875655742395	40.0	35.0	41.0	33.0	41.0
39	37.33989996994543	40.0	35.0	41.0	33.0	41.0
40	37.24858907402479	40.0	35.0	41.0	33.0	41.0
41	37.168995016274465	40.0	35.0	41.0	32.0	41.0
42	37.08470722348545	39.0	35.0	41.0	32.0	41.0
43	37.04445520952369	39.0	35.0	41.0	32.0	41.0
44	36.97682464852541	39.0	35.0	41.0	32.0	41.0
45	36.93361328712003	39.0	35.0	41.0	32.0	41.0
46	36.882313301952884	39.0	35.0	41.0	32.0	41.0
47	36.78147771823035	39.0	35.0	41.0	31.0	41.0
48	36.71751481479218	39.0	35.0	41.0	31.0	41.0
49	36.6558373919966	39.0	35.0	41.0	31.0	41.0
50	36.5721812451703	39.0	35.0	41.0	31.0	41.0
51	35.66474876656821	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	22.0
10	42.0
11	34.0
12	30.0
13	46.0
14	49.0
15	72.0
16	107.0
17	189.0
18	386.0
19	691.0
20	1153.0
21	1880.0
22	2823.0
23	3997.0
24	5677.0
25	8208.0
26	11149.0
27	13491.0
28	15248.0
29	17830.0
30	21824.0
31	27672.0
32	36769.0
33	52624.0
34	100236.0
35	134889.0
36	123855.0
37	194066.0
38	365520.0
39	658908.0
40	565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22394932610542	21.596625443943783	24.77263245260294	11.406792777347853
2	32.15355718895881	24.6364146952961	25.525607771745257	17.684420343999836
3	31.643851673750696	24.780465529185435	26.617905301992877	16.957777495070996
4	28.795278376986534	26.25913928376792	26.356469426835456	18.589112912410094
5	26.93267276239279	30.6386068456645	23.847884985992128	18.580835405950584
6	26.923228627506095	35.30523166185108	22.66359047120122	15.107949239441595
7	83.10494267132356	5.538540681166562	7.229929685638082	4.126586961871805
8	85.17104161585814	4.749677649454823	6.2537394607621195	3.82554127392491
9	79.00096607944518	7.489587841287425	8.169954429271485	5.339491649995916
10	40.19773796303344	32.79470284029579	14.96489837277556	12.042660823895217
11	28.497732574321176	25.541496139848753	27.02489196187458	18.935879323955493
12	27.99147139065997	22.617425317725697	29.680082708400114	19.711020583214218
13	24.62397065873952	23.652558055041528	31.086425500497484	20.637045785721465
14	19.97806738556903	28.111189688782424	29.47864486664048	22.432098059008066
15	19.49102779408897	27.364769710326158	33.15191335395117	19.99228914163369
16	22.37654432438048	25.68171376604878	31.169311672561843	20.772430237008898
17	21.74912044549651	25.784988158721468	28.91916320520605	23.546728190575976
18	21.769897542247225	26.98628211630841	30.100957801938716	21.14286253950565
19	23.93738205247717	27.42199005699258	27.424712189989325	21.215915700540926
20	26.470354582822008	26.70334694585011	27.91508500554704	18.911213465780843
21	25.08489999494461	26.596517114161255	28.366014669519167	19.952568221374968
22	23.37790039104274	24.78374319952846	28.08452389616118	23.75383251326762
23	22.625369501777442	26.74540112296319	28.486899596068792	22.142329779190568
24	22.70786679769941	25.52421892837957	29.55264244116443	22.215271832756592
25	22.284769554775703	27.255939944190715	27.676370607852295	22.78291989318128
26	21.380354755038585	28.739557981155063	27.787644738311357	22.092442525495
27	22.01550060303579	28.707892352417336	27.607428423179464	21.66917862136741
28	20.538382353022875	28.3115164558495	29.125156453205147	22.024944737922482
29	21.770397525858876	26.56040718665333	29.224764299392408	22.44443098809539
30	22.145218573391208	26.646182152918318	28.63078376875425	22.577815504936225
31	22.563815963810075	28.558897236146148	26.429967017747753	22.447319782296024
32	23.955881446108155	28.366459099396184	25.85026379690888	21.827395657586777
33	23.067910551820802	27.68914796681664	26.552296341397696	22.690645139964857
34	20.863816130471278	27.424489975050818	29.28915107782578	22.422542816652122
35	22.01111185800021	26.173086548829787	27.95325042123619	23.86255117193381
36	22.821307523808944	28.69028181854039	26.382579682110418	22.105830975540247
37	22.564204839952467	28.526009425246617	27.79420007899741	21.115585655803503
38	22.198439051164435	27.425878818416503	27.235273954909257	23.1404081755098
39	23.186684436454584	25.638937390385536	28.738669121401024	22.43570905175886
40	22.71586653548578	25.176008119733854	30.190732637096897	21.91739270768347
41	20.53660463351479	26.841286868930407	28.429845910606268	24.19226258694854
42	20.922369766768757	27.314938010365214	27.8078662977158	23.95482592515023
43	23.169796101127798	26.12042160840284	27.627094445237628	23.08268784523174
44	22.260103696601057	26.549129778523927	27.66759311778114	23.52317340709388
45	22.085609416135803	25.949593874423005	27.30116068417757	24.663636025263617
46	21.248359081563436	26.595794915611098	28.40779107795911	23.748054924866352
47	20.904037034341652	26.23975103038289	29.956518091906986	22.899693843368468
48	21.42329779190571	24.89957273622698	28.97955011474624	24.69757935712107
49	21.29363537528492	25.251005661481095	30.069125511997107	23.386233451236876
50	20.495939299767397	25.130065181196837	29.5197546302649	24.854240888770867
51	19.88390380537527	25.115010119112764	27.984249405158383	27.016836670353584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	483.0
1	1215.5
2	1948.0
3	4999.5
4	8051.0
5	5715.0
6	3379.0
7	3230.5
8	3082.0
9	3111.5
10	3141.0
11	3018.5
12	2896.0
13	2903.5
14	2911.0
15	3263.5
16	3616.0
17	3380.0
18	3144.0
19	3480.0
20	3816.0
21	4636.5
22	5457.0
23	7405.0
24	9353.0
25	11405.0
26	15309.5
27	17162.0
28	22059.0
29	26956.0
30	32198.5
31	37441.0
32	40803.5
33	44166.0
34	51941.0
35	59716.0
36	65071.0
37	70426.0
38	73686.5
39	76947.0
40	83413.5
41	89880.0
42	95393.5
43	100907.0
44	112295.0
45	123683.0
46	134885.0
47	146087.0
48	153499.0
49	160911.0
50	155670.5
51	150430.0
52	135483.5
53	120537.0
54	106149.0
55	91761.0
56	87804.5
57	83848.0
58	80706.0
59	77564.0
60	76723.5
61	75883.0
62	69234.5
63	62586.0
64	53237.0
65	43888.0
66	35317.5
67	26747.0
68	21904.5
69	17062.0
70	14327.5
71	11593.0
72	9722.0
73	7851.0
74	6297.5
75	4126.5
76	3509.0
77	2479.0
78	1449.0
79	1178.0
80	907.0
81	675.5
82	444.0
83	298.0
84	152.0
85	107.0
86	62.0
87	40.5
88	19.0
89	11.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1800059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.747399286945274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.64394152431817	14.864254612955877
2	11.363581453287381	4.715295234821583
3	4.448490769216452	2.7688384263967216
4	2.2777342172799324	1.8902824510177811
5	1.3474799881972592	1.3978352673148422
6	0.8460569534646686	1.0532088859816773
7	0.6209336616635444	0.9017931026466977
8	0.4429763849950203	0.7352486347343423
9	0.3729299089727468	0.696359315475156
>10	3.8719054751725928	20.3504840883338
>50	2.106585280947747	31.833900223460944
>100	0.6500831162265278	17.00837505091959
>500	0.005137928107429021	0.7791799973619005
>1k	0.00216333815049643	1.0049447085790872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4079	0.2266036835459282	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	3733	0.20738209136478303	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	3489	0.19382698011565178	No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	1998	0.11099636178591925	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1110746925517441E-4	0.0	0.0	0.11777391741048487	0.0
2	1.1110746925517441E-4	0.0	0.0	0.6588117389485567	0.0
3	1.1110746925517441E-4	0.0	0.0	0.9582463685912518	0.0
4	1.1110746925517441E-4	0.0	0.0	1.289791056848692	0.0
5	1.1110746925517441E-4	0.0	0.0	2.264148008481944	0.0
6	1.1110746925517441E-4	0.0	0.0	2.7651871410881532	0.0
7	1.1110746925517441E-4	0.0	0.0	3.2731704905228107	0.0
8	1.1110746925517441E-4	0.0	0.0	4.072088748202142	0.0
9	1.1110746925517441E-4	0.0	0.0	4.356523869495389	0.0
10	1.666612038827616E-4	0.0	0.0	5.089999827783423	0.0
11	1.666612038827616E-4	0.0	0.0	6.132965641681745	0.0
12	1.666612038827616E-4	0.0	0.0	7.0074925321892225	0.0
13	1.666612038827616E-4	0.0	0.0	7.337537269611718	0.0
14	1.666612038827616E-4	0.0	0.0	7.464310892031873	0.0
15	1.666612038827616E-4	0.0	0.0	7.710858366309104	0.0
16	1.666612038827616E-4	0.0	0.0	8.266895696196624	0.0
17	1.666612038827616E-4	0.0	0.0	8.946040102018879	0.0
18	1.666612038827616E-4	0.0	0.0	9.649072613730995	0.0
19	1.666612038827616E-4	0.0	0.0	10.10183555094583	0.0
20	1.666612038827616E-4	0.0	0.0	10.537043508018348	0.0
21	1.666612038827616E-4	0.0	0.0	11.07391479945935	0.0
22	1.666612038827616E-4	0.0	0.0	11.665117643366134	0.0
23	1.666612038827616E-4	0.0	0.0	12.249542931648351	0.0
24	1.666612038827616E-4	0.0	0.0	12.724971792591242	0.0
25	1.666612038827616E-4	0.0	0.0	13.133402849573264	0.0
26	1.666612038827616E-4	0.0	0.0	13.508057235901711	0.0
27	1.666612038827616E-4	0.0	0.0	13.883322713311063	0.0
28	1.666612038827616E-4	0.0	0.0	14.268532309218754	0.0
29	2.2221493851034882E-4	0.0	0.0	14.682796508336672	0.0
30	2.2221493851034882E-4	0.0	0.0	15.154281054121004	0.0
31	2.2221493851034882E-4	0.0	0.0	15.581822595814915	0.0
32	2.2221493851034882E-4	0.0	0.0	16.005030946207874	0.0
33	2.2221493851034882E-4	0.0	0.0	16.397129205209385	0.0
34	2.7776867313793603E-4	0.0	0.0	16.81511550454735	0.0
35	2.7776867313793603E-4	0.0	0.0	17.242823707445144	0.0
36	2.7776867313793603E-4	0.0	0.0	17.675642853928675	0.0
37	3.333224077655232E-4	0.0	0.0	18.095073550366962	0.0
38	3.333224077655232E-4	0.0	0.0	18.5097821793619	0.0
39	3.333224077655232E-4	0.0	0.0	18.943212416926333	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAC	20	7.034351E-4	45.000004	29
CGTACCC	20	7.034351E-4	45.000004	31
CGAACCG	50	2.1827873E-11	45.0	2
AAACGCG	25	3.89169E-5	45.0	1
TCGAACG	25	3.89169E-5	45.0	1
CTATCGT	30	2.1661908E-6	44.999996	1
TACGCTA	30	2.1661908E-6	44.999996	17
TTGCGAG	150	0.0	40.500004	1
TGTCGCG	45	1.929402E-8	40.0	1
GCGTAAG	185	0.0	38.91892	1
CGCCTAG	35	6.2502586E-6	38.57143	33
CGTAAGG	310	0.0	38.467743	2
CGTTTTT	2585	0.0	38.03675	1
ACGCGTA	95	0.0	37.894737	24
ACTATCG	30	1.1401788E-4	37.499996	1
ATATCGC	30	1.1401788E-4	37.499996	34
CGTAGTG	30	1.1401788E-4	37.499996	27
GTATACG	60	1.5643309E-10	37.499996	1
CCGGTAT	30	1.1401788E-4	37.499996	42
ATAGACG	115	0.0	37.173912	1
>>END_MODULE