FastQCFastQC Report
Sat 18 Jun 2016
SRR3549842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344243
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60421.755155515144825No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC23560.6844002637671645Illumina PCR Primer Index 11 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19930.578951496471969No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC18800.5461258471486712No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC17710.5144621677129237Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT15080.4380626476064873Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC11600.3369712673896056Illumina PCR Primer Index 11 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10830.3146033470542611No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.23762284200404948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7900.22948905279119694No Hit
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC7030.2042162077369765Illumina PCR Primer Index 11 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.18678665942372102No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG5860.17022858852612835No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.1519275627972101No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.12839767257431525No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC4390.12752619515865246No Hit
ACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG4330.1257832403273269Illumina PCR Primer Index 11 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4060.11793994358636195No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC3660.10632024471085831No Hit
GCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG3560.1034153199919824Illumina PCR Primer Index 11 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG3550.10312482752009482Illumina PCR Primer Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG207.026392E-445.0000041
GCGAATG207.026392E-445.0000041
GGCCGAT253.8850914E-545.0000048
TAACGAG207.026392E-445.0000041
TATGCGG406.7902874E-945.0000042
ATCGTAG207.026392E-445.0000041
TGCGAAG207.026392E-445.0000041
GTAAGTC253.8850914E-545.00000419
GCGCTTA207.026392E-445.00000430
TATACGG207.026392E-445.0000042
ACAAGTA207.026392E-445.00000419
AGTAACG253.8850914E-545.0000041
TAACTAG207.026392E-445.00000424
CACGGGC302.1610558E-644.9999964
ATCCGCT302.1610558E-644.9999961
CGCTAGT302.1610558E-644.9999964
GCTACGA600.044.99999610
CGTTATT1500.043.51
AGCATGG800.042.1875042
CGTTTTT22200.042.1621671
Produced by FastQC (version 0.11.5)