##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	344243
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60295779434876	31.0	31.0	34.0	30.0	34.0
2	31.699624974218793	31.0	31.0	34.0	30.0	34.0
3	31.719390082006026	31.0	31.0	34.0	30.0	34.0
4	35.52916108679044	37.0	35.0	37.0	33.0	37.0
5	32.94064657814393	37.0	35.0	37.0	28.0	37.0
6	33.99035855485805	37.0	35.0	37.0	26.0	37.0
7	35.31956205354938	37.0	35.0	37.0	32.0	37.0
8	35.26446434640646	37.0	35.0	37.0	33.0	37.0
9	37.297548534029744	39.0	37.0	39.0	34.0	39.0
10	36.763945817344144	39.0	37.0	39.0	32.0	39.0
11	36.592895135122575	39.0	35.0	39.0	32.0	39.0
12	36.96064117498395	39.0	37.0	39.0	33.0	39.0
13	36.91911527612762	39.0	37.0	39.0	33.0	39.0
14	37.850381852354296	40.0	37.0	41.0	33.0	41.0
15	37.9935365425005	40.0	37.0	41.0	33.0	41.0
16	37.9587094000459	40.0	37.0	41.0	33.0	41.0
17	37.94076858498212	39.0	37.0	41.0	33.0	41.0
18	37.90486952530625	39.0	37.0	40.0	33.0	41.0
19	37.742850254035666	39.0	37.0	41.0	33.0	41.0
20	37.601209029668	39.0	35.0	40.0	33.0	41.0
21	37.52742394180855	39.0	35.0	40.0	33.0	41.0
22	37.50125347501619	39.0	35.0	40.0	33.0	41.0
23	37.25622888482845	39.0	35.0	40.0	32.0	41.0
24	36.97363490325148	39.0	35.0	40.0	32.0	41.0
25	36.784094375194265	39.0	35.0	40.0	31.0	41.0
26	37.09795115659578	39.0	35.0	40.0	32.0	41.0
27	37.00887164009145	39.0	35.0	40.0	32.0	41.0
28	37.07004644974626	39.0	35.0	40.0	32.0	41.0
29	37.1751640556235	39.0	35.0	40.0	33.0	41.0
30	36.92263023503746	39.0	35.0	40.0	32.0	41.0
31	36.7464872197837	39.0	35.0	40.0	31.0	41.0
32	36.59775507417725	39.0	35.0	40.0	31.0	41.0
33	36.48027991854591	39.0	35.0	40.0	31.0	41.0
34	36.3384789233187	39.0	35.0	40.0	30.0	41.0
35	36.26058336698205	39.0	35.0	40.0	30.0	41.0
36	36.11875041758293	39.0	35.0	40.0	30.0	41.0
37	36.00291073456831	39.0	35.0	40.0	29.0	41.0
38	35.924451622836195	39.0	35.0	40.0	29.0	41.0
39	35.887384783423336	39.0	35.0	40.0	29.0	41.0
40	35.79177499615098	39.0	35.0	40.0	27.0	41.0
41	35.73995404409095	39.0	35.0	40.0	27.0	41.0
42	35.83333865902865	39.0	35.0	40.0	27.0	41.0
43	35.74409356181535	39.0	35.0	40.0	27.0	41.0
44	35.64243281635356	39.0	35.0	40.0	26.0	41.0
45	35.62706866951543	39.0	35.0	40.0	26.0	41.0
46	35.519833373518125	39.0	35.0	40.0	25.0	41.0
47	35.451936568063836	39.0	35.0	40.0	25.0	41.0
48	35.44066255522988	39.0	35.0	40.0	25.0	41.0
49	35.46066005699462	39.0	35.0	40.0	26.0	41.0
50	35.40076631914084	39.0	35.0	40.0	26.0	41.0
51	34.472459861202694	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	6.0
11	6.0
12	13.0
13	12.0
14	19.0
15	21.0
16	50.0
17	89.0
18	132.0
19	222.0
20	391.0
21	676.0
22	952.0
23	1394.0
24	2140.0
25	3121.0
26	4537.0
27	5374.0
28	5697.0
29	5921.0
30	6436.0
31	7697.0
32	9909.0
33	13498.0
34	20626.0
35	28079.0
36	36462.0
37	50369.0
38	76769.0
39	63601.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.462739983093336	23.497064573571578	24.218647873740352	16.821547569594735
2	35.73231699700502	24.38626203001949	24.322353686204224	15.559067286771263
3	27.552629973594232	23.981896509151966	33.88042748872163	14.58504602853217
4	25.048294373451313	25.664138413853006	32.77103673858292	16.516530474112763
5	19.62073302870356	32.93865089486206	30.1075112638456	17.333104812588783
6	21.52955906147692	32.88287634025964	32.127886405823794	13.459678192439641
7	66.99511682154757	7.704150846930801	19.550433850506764	5.750298481014864
8	64.22323765479618	7.382575680551239	20.270564688316103	8.123621976336484
9	58.76604607791589	9.643769081724248	23.277452264824557	8.312732575535305
10	29.067838706959908	28.969652251461902	28.535075513518066	13.42743352806012
11	19.705847322966623	23.685594187826624	36.42775597470392	20.180802514502837
12	19.491754371185472	20.28189389471972	41.79954276484925	18.426808969245563
13	20.22263343045465	22.160218217944884	41.71733339530506	15.899814956295408
14	14.910978581990047	26.65239380321459	38.32844821826442	20.10817939653094
15	13.45764474513643	25.75738649732892	42.65678604938953	18.12818270814512
16	13.084652411232764	26.72617889107404	38.901299372826756	21.287869324866445
17	13.837899390837286	26.4766458577226	36.27844284415369	23.407011907286424
18	13.49250384176294	26.230598734033805	40.58005536786514	19.69684205633811
19	15.236039658032263	26.830756180953568	38.051027907611775	19.882176253402392
20	15.731038830128718	28.66492564845182	37.91623940065593	17.68779612076353
21	15.439965373297351	27.44079037191751	39.28852583785291	17.830718416932225
22	12.964969512815076	26.488556049069988	35.77327643554117	24.773198002573764
23	13.965425585995938	25.861092309792795	36.727544205691906	23.445937898519357
24	16.610069050060567	24.401077146085758	36.331893458981014	22.65696034487266
25	13.999413205206787	27.366133806642402	33.91790101759513	24.716551970555685
26	12.751748038449584	27.890763210871388	36.56806383862562	22.789424912053406
27	15.850721728546405	26.569893941198515	37.23212962936066	20.34725470089443
28	11.366389440017661	28.342479004656596	35.6204773953283	24.670654159997444
29	14.549896439433772	25.209517695348925	34.85909662651092	25.38148923870638
30	15.971276104379756	27.81726861548383	33.422030368083014	22.789424912053406
31	13.74552278477703	34.02073535264334	29.299361207054318	22.934380655525313
32	13.083199948873325	32.30595829109089	28.654467919463865	25.95637384057192
33	14.983892192433835	28.509802668463845	29.451579262323417	27.0547258767789
34	12.966712467646403	30.29023102866289	29.093692536957903	27.649363966732803
35	13.202011369875349	26.814198110055976	28.156563822648533	31.827226697420137
36	13.385893104580196	29.08236333055429	29.67061058612666	27.861132978738855
37	13.868401100385483	33.08912599529983	30.96533553332965	22.07713737098503
38	13.628744811078223	32.897110471382135	26.42784312244548	27.046301595094164
39	17.174786415410047	27.281890989795	27.52648565112435	28.016836943670604
40	16.843334504986306	26.879268423758802	29.440831040863575	26.836566030391324
41	14.685556423805277	26.633511792541896	27.118634220594174	31.562297563058657
42	15.540185276098569	25.503496076899168	29.677582405451965	29.278736241550302
43	17.91931862085794	24.99077686401757	31.450748453853816	25.639156061270675
44	16.46511330658866	26.706134910513796	28.36891381959836	28.459837963299183
45	18.312064442849962	24.493163259674127	27.756265196387435	29.438507101088472
46	18.432618818683313	25.81693745406588	29.71302248702225	26.037421240228557
47	14.043858553405588	26.441786761096086	33.137928730576945	26.37642595492138
48	13.961649183861399	24.430126393274517	32.176398648629025	29.431825774235058
49	17.76361465592619	22.142788669631628	33.73401928289028	26.3595773915519
50	16.56271877714289	21.248652841161622	32.28649529547442	29.90213308622107
51	15.884709347757253	20.38792364695869	28.832249312259073	34.895117693024986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1392.0
1	2698.0
2	4004.0
3	7410.5
4	10817.0
5	7017.5
6	3218.0
7	3123.5
8	3029.0
9	3074.5
10	3120.0
11	3131.0
12	3142.0
13	3010.5
14	2879.0
15	2954.5
16	3030.0
17	2914.0
18	2798.0
19	3266.5
20	3735.0
21	3532.5
22	3330.0
23	3442.5
24	3555.0
25	4621.0
26	5619.5
27	5552.0
28	5729.5
29	5907.0
30	6191.5
31	6476.0
32	6223.5
33	5971.0
34	6406.0
35	6841.0
36	7284.5
37	7728.0
38	8358.0
39	8988.0
40	10401.0
41	11814.0
42	14379.0
43	16944.0
44	19974.5
45	23005.0
46	29009.0
47	35013.0
48	40548.5
49	46084.0
50	44012.5
51	41941.0
52	32601.0
53	23261.0
54	17905.0
55	12549.0
56	10013.5
57	7478.0
58	6808.5
59	6139.0
60	6029.5
61	5920.0
62	5326.0
63	4732.0
64	3907.5
65	3083.0
66	2548.5
67	2014.0
68	1595.0
69	1176.0
70	944.0
71	712.0
72	630.0
73	548.0
74	410.5
75	213.0
76	153.0
77	135.0
78	117.0
79	87.5
80	58.0
81	37.0
82	16.0
83	10.5
84	5.0
85	6.0
86	7.0
87	4.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	344243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.6313397754434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0877182795002	31.016523901138715
2	11.680217077871543	10.192470399510999
3	5.323935325427047	6.968712933785799
4	3.1567218953868466	5.509280223768209
5	2.1060488967180433	4.594486749820132
6	1.537246303848603	4.024326948105775
7	1.1255535260935503	3.437658583270527
8	0.9023416195846009	3.149629903809569
9	0.6404021398675191	2.5147443021732685
>10	2.3013880145924492	15.617600208119974
>50	0.08035513957094535	2.5216701276208706
>100	0.04861823570679046	4.013636189809454
>500	0.004051519642232539	1.1970493899431707
>1k	0.0047267729159379615	3.4621037118440063
>5k	6.752532737054231E-4	1.7801064272795069
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6042	1.755155515144825	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	2356	0.6844002637671645	Illumina PCR Primer Index 11 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1993	0.578951496471969	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	1880	0.5461258471486712	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1771	0.5144621677129237	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1508	0.4380626476064873	Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1160	0.3369712673896056	Illumina PCR Primer Index 11 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1083	0.3146033470542611	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	818	0.23762284200404948	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	790	0.22948905279119694	No Hit
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	703	0.2042162077369765	Illumina PCR Primer Index 11 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	643	0.18678665942372102	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	586	0.17022858852612835	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	523	0.1519275627972101	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	442	0.12839767257431525	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	439	0.12752619515865246	No Hit
ACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	433	0.1257832403273269	Illumina PCR Primer Index 11 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	406	0.11793994358636195	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC	366	0.10632024471085831	No Hit
GCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	356	0.1034153199919824	Illumina PCR Primer Index 11 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	355	0.10312482752009482	Illumina PCR Primer Index 11 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4694358345703471	0.0
2	0.0	0.0	0.0	2.315805985887876	0.0
3	0.0	0.0	0.0	3.1201796405446154	0.0
4	0.0	0.0	0.0	4.28214952809498	0.0
5	0.0	0.0	0.0	7.308209607748015	0.0
6	0.0	0.0	0.0	9.093285847497262	0.0
7	0.0	0.0	0.0	10.995430553417208	0.0
8	0.0	0.0	0.0	13.757142483652537	0.0
9	0.0	0.0	0.0	14.779675984696857	0.0
10	0.0	0.0	0.0	17.42983880572735	0.0
11	0.0	0.0	0.0	21.854620137519134	0.0
12	0.0	0.0	0.0	24.991648341433233	0.0
13	0.0	0.0	0.0	26.209683276057902	0.0
14	0.0	0.0	0.0	26.714559192198536	0.0
15	0.0	0.0	0.0	27.683061093471764	0.0
16	0.0	0.0	0.0	29.77402590611864	0.0
17	0.0	0.0	0.0	32.25599358592622	0.0
18	0.0	0.0	0.0	34.802450594492846	0.0
19	0.0	0.0	0.0	36.45680522189267	0.0
20	0.0	0.0	0.0	37.97288543267401	0.0
21	0.0	0.0	0.0	39.7289124252345	0.0
22	0.0	0.0	0.0	41.645872247220716	0.0
23	0.0	0.0	0.0	43.29325505529524	0.0
24	0.0	0.0	0.0	44.41687993655644	0.0
25	0.0	0.0	0.0	45.27441371356861	0.0
26	0.0	0.0	0.0	45.94167492149441	0.0
27	0.0	0.0	0.0	46.570300630659155	0.0
28	0.0	0.0	0.0	47.14925212713113	0.0
29	0.0	0.0	0.0	47.73953283000671	0.0
30	0.0	0.0	0.0	48.32865156299474	0.0
31	2.90492471887591E-4	0.0	0.0	48.8588003241896	0.0
32	2.90492471887591E-4	0.0	0.0	49.32533123404107	0.0
33	2.90492471887591E-4	0.0	0.0	49.73085872479615	0.0
34	2.90492471887591E-4	0.0	0.0	50.089035942633544	0.0
35	2.90492471887591E-4	0.0	0.0	50.483524719456895	0.0
36	2.90492471887591E-4	0.0	0.0	50.857098038304336	0.0
37	2.90492471887591E-4	0.0	0.0	51.23328578939877	0.0
38	2.90492471887591E-4	0.0	0.0	51.58565315779842	0.0
39	2.90492471887591E-4	0.0	0.0	51.92959624451332	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	7.026392E-4	45.000004	1
GCGAATG	20	7.026392E-4	45.000004	1
GGCCGAT	25	3.8850914E-5	45.000004	8
TAACGAG	20	7.026392E-4	45.000004	1
TATGCGG	40	6.7902874E-9	45.000004	2
ATCGTAG	20	7.026392E-4	45.000004	1
TGCGAAG	20	7.026392E-4	45.000004	1
GTAAGTC	25	3.8850914E-5	45.000004	19
GCGCTTA	20	7.026392E-4	45.000004	30
TATACGG	20	7.026392E-4	45.000004	2
ACAAGTA	20	7.026392E-4	45.000004	19
AGTAACG	25	3.8850914E-5	45.000004	1
TAACTAG	20	7.026392E-4	45.000004	24
CACGGGC	30	2.1610558E-6	44.999996	4
ATCCGCT	30	2.1610558E-6	44.999996	1
CGCTAGT	30	2.1610558E-6	44.999996	4
GCTACGA	60	0.0	44.999996	10
CGTTATT	150	0.0	43.5	1
AGCATGG	80	0.0	42.187504	2
CGTTTTT	2220	0.0	42.162167	1
>>END_MODULE