SRR3549841_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285540
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3588	1.2565665055683968	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	2431	0.8513693352945297	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1880	0.6584016249912447	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	1254	0.4391678924143727	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	1246	0.43636618337185684	No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1200	0.42025635637739023	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1139	0.3988933249282062	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	778	0.27246620438467467	No Hit
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	698	0.24444911395951532	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	605	0.21187924634026756	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	547	0.19156685578202703	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	463	0.16214891083560973	No Hit
ACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	399	0.13973523849548225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	380	0.1330811795195069	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.13133011136793443	No Hit
GCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	371	0.12992925684667647	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	365	0.12782797506478952	No Hit
AGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	327	0.11451985711283884	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	310	0.10856622539749247	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC	296	0.10366323457308957	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	20	7.024372E-4	45.0	6
AGGTACG	20	7.024372E-4	45.0	1
CGTATGG	25	3.883418E-5	45.0	2
TTTACGG	20	7.024372E-4	45.0	2
CGACGGG	20	7.024372E-4	45.0	3
GGCGTAT	25	3.883418E-5	45.0	6
GACAAGG	25	3.883418E-5	45.0	2
TTCACAC	20	7.024372E-4	45.0	15
TTCAAGT	25	3.883418E-5	45.0	34
AGGCGTC	20	7.024372E-4	45.0	35
AGGCGTA	25	3.883418E-5	45.0	5
CGTTCTG	20	7.024372E-4	45.0	1
ACGGGTC	25	3.883418E-5	45.0	5
ACGGGCA	45	3.8198777E-10	45.0	5
CAGGCGT	20	7.024372E-4	45.0	34
CGGGCGA	20	7.024372E-4	45.0	6
GACGGGT	40	6.7848305E-9	45.0	4
GCCTAAT	20	7.024372E-4	45.0	9
CGTTTTC	25	3.883418E-5	45.0	1
GCGTCAT	20	7.024372E-4	45.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content