##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285540
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.487812565665056	31.0	31.0	34.0	30.0	34.0
2	31.585252504027455	31.0	31.0	34.0	30.0	34.0
3	31.63243678643973	31.0	31.0	34.0	30.0	34.0
4	35.45697625551586	37.0	35.0	37.0	33.0	37.0
5	32.92507529593052	35.0	35.0	37.0	28.0	37.0
6	33.90435315542481	36.0	35.0	37.0	26.0	37.0
7	35.25742803109897	37.0	35.0	37.0	32.0	37.0
8	35.21794494641731	37.0	35.0	37.0	33.0	37.0
9	37.20699726833368	39.0	37.0	39.0	34.0	39.0
10	36.54434404987042	39.0	35.0	39.0	32.0	39.0
11	36.418214610912656	39.0	35.0	39.0	32.0	39.0
12	36.84946067100932	39.0	37.0	39.0	33.0	39.0
13	36.80131330111368	39.0	37.0	39.0	33.0	39.0
14	37.66340967990474	39.0	37.0	41.0	33.0	41.0
15	37.80937872101982	39.0	37.0	41.0	33.0	41.0
16	37.81716747215802	39.0	37.0	41.0	33.0	41.0
17	37.80925264411291	39.0	37.0	41.0	33.0	41.0
18	37.7845205575401	39.0	37.0	40.0	33.0	41.0
19	37.66912516635148	39.0	37.0	40.0	33.0	41.0
20	37.55192967710303	39.0	36.0	40.0	33.0	41.0
21	37.46604678854101	39.0	36.0	40.0	33.0	41.0
22	37.43433144217973	39.0	35.0	40.0	33.0	41.0
23	37.20824753099391	39.0	35.0	40.0	32.0	41.0
24	36.91642501926175	39.0	35.0	40.0	32.0	41.0
25	36.738968270645096	39.0	35.0	40.0	31.0	41.0
26	37.07818169083141	39.0	35.0	40.0	32.0	41.0
27	36.99697415423408	39.0	35.0	40.0	32.0	41.0
28	37.128822581774884	39.0	35.0	40.0	32.0	41.0
29	37.230472087973666	39.0	35.0	40.0	33.0	41.0
30	37.02087623450305	39.0	35.0	40.0	32.0	41.0
31	36.908569727533795	39.0	35.0	40.0	31.0	41.0
32	36.70532324718078	39.0	35.0	40.0	31.0	41.0
33	36.72209497793654	39.0	35.0	40.0	31.0	41.0
34	36.726721299992995	39.0	35.0	40.0	31.0	41.0
35	36.514694963927994	39.0	35.0	40.0	30.0	41.0
36	36.42794004342649	39.0	35.0	40.0	30.0	41.0
37	36.3664810534426	39.0	35.0	40.0	30.0	41.0
38	36.19839251943686	39.0	35.0	40.0	30.0	41.0
39	36.20952230860825	39.0	35.0	40.0	30.0	41.0
40	36.14580093857253	39.0	35.0	40.0	30.0	41.0
41	36.01327309658892	39.0	35.0	40.0	29.0	41.0
42	36.130128878615956	39.0	35.0	40.0	30.0	41.0
43	36.06411711143798	39.0	35.0	40.0	29.0	41.0
44	35.97909574840653	39.0	35.0	40.0	29.0	41.0
45	35.97442389857813	39.0	35.0	40.0	29.0	41.0
46	35.85884289416544	39.0	35.0	40.0	28.0	41.0
47	35.77831126987462	39.0	35.0	40.0	28.0	41.0
48	35.734338446452334	39.0	35.0	40.0	27.0	41.0
49	35.76816908314071	39.0	35.0	40.0	27.0	41.0
50	35.6973278700007	39.0	35.0	40.0	27.0	41.0
51	34.72741822511732	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	4.0
11	4.0
12	4.0
13	9.0
14	12.0
15	16.0
16	22.0
17	54.0
18	97.0
19	193.0
20	307.0
21	516.0
22	701.0
23	1131.0
24	1735.0
25	2434.0
26	3289.0
27	3796.0
28	4141.0
29	4445.0
30	5181.0
31	6361.0
32	8344.0
33	11488.0
34	17076.0
35	23150.0
36	30668.0
37	44689.0
38	67355.0
39	48299.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.050500805491346	22.5471037332773	24.435805841563354	15.966589619667998
2	32.54430202423478	24.758352595083	26.660362821321005	16.03698255936121
3	28.17398613154024	24.62036842473909	32.93934299922953	14.266302444491139
4	25.81985010856623	25.031169013097994	32.69839602157316	16.450584856762624
5	19.680955382783498	32.35273516845275	30.309939062828324	17.65637038593542
6	20.655950129579043	34.03796315752609	30.97604538768649	14.330041325208375
7	68.45415703579182	7.092526441129089	18.007634657140855	6.445681865938223
8	65.09210618477272	6.8375709182601385	18.764446312250474	9.305876584716676
9	60.395741402255375	8.024444911395952	21.974504447713105	9.605309238635568
10	29.108005883588987	24.161588569027106	29.984240386635847	16.746165160748056
11	19.157386005463334	25.077747425929818	34.28206205785529	21.482804510751556
12	19.59690411150802	19.554528262239966	40.77187084121314	20.076696785038877
13	19.92575471037333	22.51593472017931	41.34797226308048	16.210338306366882
14	14.904391678924146	26.43202353435596	37.33767598234923	21.325908804370666
15	12.1096869090145	25.333403376059394	43.51824613013939	19.03866358478672
16	12.129649085942425	26.265672059956575	38.143167332072565	23.461511522028438
17	12.808713315122224	26.469496392799606	35.346711494011345	25.37507879806682
18	12.954752398963368	25.527071513623312	40.87273236674371	20.64544372066961
19	14.28276248511592	26.7205995657351	37.03614204664846	21.960495902500526
20	14.986691882048051	28.607200392239267	37.07186383694053	19.33424388877215
21	14.686208587238214	27.85038873712965	37.99152483014639	19.471877845485746
22	11.938082230160397	26.632345730895846	33.532254675351965	27.89731736359179
23	13.152623100091054	25.865027666876795	35.589409539819286	25.392939693212863
24	16.39244939413042	23.490229039714226	35.31379141276179	24.80353015339357
25	13.422988022693843	27.317713805421306	32.23191146599426	27.027386705890592
26	12.418925544582194	26.876094417594732	35.3456608531204	25.359319184702667
27	16.42046648455558	25.34776213490229	33.25873783007635	24.973033550465786
28	10.952931288085733	24.444561182321216	34.043916789241436	30.558590740351615
29	14.360860124676053	23.508790362120894	31.885550185613226	30.244799327589828
30	16.974154234082793	24.962527141556347	30.20312390558241	27.86019471877845
31	15.522868950059538	28.682846536387196	25.88253834839252	29.911746165160746
32	13.972823422287595	28.75814246690481	28.017090425159346	29.251943685648246
33	15.321846326259017	24.138124255796036	31.48665686068502	29.053372557259927
34	16.452335924914195	22.058555718988583	30.74945716887301	30.739651187224208
35	14.303425089304476	22.643062267983467	30.74280310989704	32.31070953281502
36	15.484345450724943	21.89080339006794	33.06892204244589	29.555929116761227
37	16.845275618127058	23.9293969321286	32.36954542270785	26.85578202703649
38	14.04496743013238	27.162218953561673	33.002031239055825	25.79078237725012
39	20.51061147299853	21.296140645793933	35.740001400854524	22.453246480353016
40	22.718007984870773	18.876164460320798	36.10982699446662	22.29600056034181
41	18.152623100091056	20.367374098199903	33.429291868039506	28.05071093366954
42	17.31105974644533	19.520907753729773	36.305946627442744	26.862085872382153
43	19.26560201723051	20.209427750928064	33.698255936121036	26.82671429572039
44	17.99642782097079	21.48210408349093	29.845906002661625	30.675562092876657
45	21.629193808223015	18.244028857603137	29.4368564824543	30.68992085171955
46	22.85459130069342	19.623170133781606	33.09658891924074	24.425649646284235
47	16.799047418925543	20.067241017020383	38.017090425159346	25.116621138894722
48	16.215941724451916	18.68950059536317	37.33347341878546	27.761084261399454
49	20.244449113959515	15.992155214680956	39.23863556769629	24.524760103663233
50	18.919240736849478	15.101912166421517	36.98045807942845	28.998389017300553
51	18.142466904811936	14.826644252994326	32.62345030468586	34.40743853750788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	717.0
1	1460.0
2	2203.0
3	4202.5
4	6202.0
5	4116.0
6	2030.0
7	2010.0
8	1990.0
9	1976.5
10	1963.0
11	2050.5
12	2138.0
13	2091.5
14	2045.0
15	1841.0
16	1637.0
17	1563.0
18	1489.0
19	1555.0
20	1621.0
21	1767.0
22	1913.0
23	1873.5
24	1834.0
25	2546.0
26	3295.0
27	3332.0
28	3493.0
29	3654.0
30	3883.0
31	4112.0
32	4181.5
33	4251.0
34	4504.0
35	4757.0
36	4900.5
37	5044.0
38	5645.5
39	6247.0
40	7385.0
41	8523.0
42	10938.5
43	13354.0
44	16881.0
45	20408.0
46	28129.0
47	35850.0
48	41181.0
49	46512.0
50	44333.5
51	42155.0
52	32639.0
53	23123.0
54	17356.5
55	11590.0
56	8775.0
57	5960.0
58	5160.0
59	4360.0
60	4038.5
61	3717.0
62	3328.5
63	2940.0
64	2276.5
65	1613.0
66	1449.5
67	1286.0
68	944.0
69	602.0
70	511.0
71	420.0
72	398.0
73	376.0
74	265.5
75	108.0
76	61.0
77	59.0
78	57.0
79	38.5
80	20.0
81	13.5
82	7.0
83	7.0
84	7.0
85	5.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	285540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.07957161265434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.93974076011355	31.979331237786727
2	12.224477540064628	11.02148421389256
3	5.535737428468416	7.486460155064789
4	3.219914123464434	5.806093972612484
5	2.153775629223611	4.854564135758772
6	1.5155236266976915	4.099149351623293
7	1.062799154608773	3.353737142003827
8	0.7644373649161225	2.75684071481001
9	0.5376000437016027	2.181130170211125
>10	1.9000201823575438	13.941014534476546
>50	0.08240476005526894	2.5391346182746255
>100	0.054936506703512626	4.54475732288642
>500	0.0031392289544864356	0.9297541837572407
>1k	0.0054936506703512624	4.506548246841603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3588	1.2565665055683968	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	2431	0.8513693352945297	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1880	0.6584016249912447	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	1254	0.4391678924143727	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	1246	0.43636618337185684	No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1200	0.42025635637739023	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1139	0.3988933249282062	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	778	0.27246620438467467	No Hit
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	698	0.24444911395951532	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	605	0.21187924634026756	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	547	0.19156685578202703	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	463	0.16214891083560973	No Hit
ACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	399	0.13973523849548225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	380	0.1330811795195069	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.13133011136793443	No Hit
GCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	371	0.12992925684667647	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	365	0.12782797506478952	No Hit
AGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	327	0.11451985711283884	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	310	0.10856622539749247	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC	296	0.10366323457308957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5021363031449183E-4	0.0	0.0	0.47243818729424947	0.0
2	3.5021363031449183E-4	0.0	0.0	2.8129158786859985	0.0
3	3.5021363031449183E-4	0.0	0.0	3.722420676612734	0.0
4	3.5021363031449183E-4	0.0	0.0	5.0483294809834	0.0
5	3.5021363031449183E-4	0.0	0.0	8.875814246690481	0.0
6	3.5021363031449183E-4	0.0	0.0	11.180219934159837	0.0
7	3.5021363031449183E-4	0.0	0.0	13.68319675001751	0.0
8	3.5021363031449183E-4	0.0	0.0	17.31000910555439	0.0
9	3.5021363031449183E-4	0.0	0.0	18.52945296630945	0.0
10	3.5021363031449183E-4	0.0	0.0	22.139805281221545	0.0
11	3.5021363031449183E-4	0.0	0.0	27.470406948238427	0.0
12	3.5021363031449183E-4	0.0	0.0	31.27407718708412	0.0
13	3.5021363031449183E-4	0.0	0.0	32.603137914127615	0.0
14	7.004272606289837E-4	0.0	0.0	33.12180430062338	0.0
15	7.004272606289837E-4	0.0	0.0	34.23688449954472	0.0
16	7.004272606289837E-4	0.0	0.0	36.755270715136234	0.0
17	7.004272606289837E-4	0.0	0.0	39.60355817048399	0.0
18	7.004272606289837E-4	0.0	0.0	42.73761994816838	0.0
19	7.004272606289837E-4	0.0	0.0	44.45646844575191	0.0
20	7.004272606289837E-4	0.0	0.0	46.151852630104365	0.0
21	7.004272606289837E-4	0.0	0.0	48.24577992575471	0.0
22	7.004272606289837E-4	0.0	0.0	50.460180710233246	0.0
23	7.004272606289837E-4	0.0	0.0	52.351334313931496	0.0
24	7.004272606289837E-4	0.0	0.0	53.671289486586815	0.0
25	7.004272606289837E-4	0.0	0.0	54.69286264621419	0.0
26	7.004272606289837E-4	0.0	0.0	55.53267493170834	0.0
27	7.004272606289837E-4	0.0	0.0	56.21173916088814	0.0
28	7.004272606289837E-4	0.0	0.0	56.83161728654479	0.0
29	7.004272606289837E-4	0.0	0.0	57.392659522308605	0.0
30	7.004272606289837E-4	0.0	0.0	57.964558380612175	0.0
31	7.004272606289837E-4	0.0	0.0	58.43524549975485	0.0
32	7.004272606289837E-4	0.0	0.0	58.85865377880507	0.0
33	7.004272606289837E-4	0.0	0.0	59.244939413041955	0.0
34	7.004272606289837E-4	0.0	0.0	59.60460881137494	0.0
35	7.004272606289837E-4	0.0	0.0	59.99824893184843	0.0
36	7.004272606289837E-4	0.0	0.0	60.360019611963295	0.0
37	7.004272606289837E-4	0.0	0.0	60.65770119773062	0.0
38	0.0010506408909434755	0.0	0.0	60.96869090144988	0.0
39	0.0010506408909434755	0.0	0.0	61.219794074385376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	20	7.024372E-4	45.0	6
AGGTACG	20	7.024372E-4	45.0	1
CGTATGG	25	3.883418E-5	45.0	2
TTTACGG	20	7.024372E-4	45.0	2
CGACGGG	20	7.024372E-4	45.0	3
GGCGTAT	25	3.883418E-5	45.0	6
GACAAGG	25	3.883418E-5	45.0	2
TTCACAC	20	7.024372E-4	45.0	15
TTCAAGT	25	3.883418E-5	45.0	34
AGGCGTC	20	7.024372E-4	45.0	35
AGGCGTA	25	3.883418E-5	45.0	5
CGTTCTG	20	7.024372E-4	45.0	1
ACGGGTC	25	3.883418E-5	45.0	5
ACGGGCA	45	3.8198777E-10	45.0	5
CAGGCGT	20	7.024372E-4	45.0	34
CGGGCGA	20	7.024372E-4	45.0	6
GACGGGT	40	6.7848305E-9	45.0	4
GCCTAAT	20	7.024372E-4	45.0	9
CGTTTTC	25	3.883418E-5	45.0	1
GCGTCAT	20	7.024372E-4	45.0	37
>>END_MODULE