SRR3549839_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549839_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380201
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	6880	0.4984781202158236	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	5395	0.39088509572156516	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4704	0.34081992405453987	No Hit
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	3897	0.2823501794303873	TruSeq Adapter, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2629	0.19047950262316865	TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1868	0.13534260589580793	TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTG	1683	0.12193876109349291	TruSeq Adapter, Index 14 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	20	7.033778E-4	45.000004	34
CAATTCG	30	2.1658198E-6	45.000004	15
CGCGAAA	20	7.033778E-4	45.000004	37
AAACGCG	20	7.033778E-4	45.000004	1
GTCGTAG	20	7.033778E-4	45.000004	1
CTCGACG	20	7.033778E-4	45.000004	15
CCGCGAA	20	7.033778E-4	45.000004	36
TAAGCGA	20	7.033778E-4	45.000004	37
TATCGCG	20	7.033778E-4	45.000004	1
TTCGAAC	20	7.033778E-4	45.000004	36
CCTGATA	20	7.033778E-4	45.000004	30
ATACCGG	30	2.1658198E-6	45.000004	2
ATCGCAC	20	7.033778E-4	45.000004	12
TCGAACG	30	2.1658198E-6	45.000004	1
AATCGGC	20	7.033778E-4	45.000004	11
CGAAGTC	30	2.1658198E-6	45.000004	14
CGGACGA	20	7.033778E-4	45.000004	42
TAGGTCG	25	3.891215E-5	45.0	1
CAATGCG	25	3.891215E-5	45.0	45
TGTCGCG	25	3.891215E-5	45.0	1