SRR3549838_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1204477
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	7843	0.6511539863359781	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	6286	0.5218862626683615	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4667	0.3874710766581678	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	4551	0.37784034066237876	TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2798	0.23229999410532537	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2196	0.18231979523062708	TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1903	0.15799388448264268	TruSeq Adapter, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1544	0.128188417047399	TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1426	0.11839163387926877	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1290	0.10710042616006782	TruSeq Adapter, Index 27 (95% over 22bp)
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1242	0.10311529402387924	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.1655887E-6	45.000004	1
TTAGCCG	25	3.8909166E-5	45.000004	1
TACGGAC	30	2.1655887E-6	45.000004	14
CGCCTAC	30	2.1655887E-6	45.000004	29
TACGCGC	35	1.2119926E-7	45.000004	34
CGTAGGT	25	3.8909166E-5	45.000004	28
CGTACTA	35	1.2119926E-7	45.000004	18
GTTCACG	25	3.8909166E-5	45.000004	1
GGACCGT	25	3.8909166E-5	45.000004	8
GCGTAAG	65	0.0	45.000004	1
CCGCAAT	25	3.8909166E-5	45.000004	43
GTACGTC	45	3.8562575E-10	45.000004	1
TACGTAG	35	1.2119926E-7	45.000004	1
CTCGTCG	20	7.0334214E-4	45.0	31
ATTAGCG	20	7.0334214E-4	45.0	1
CGTATCA	20	7.0334214E-4	45.0	18
CTATCGT	20	7.0334214E-4	45.0	44
CGTGCCA	20	7.0334214E-4	45.0	21
CGACCTA	20	7.0334214E-4	45.0	38
CCTACGC	20	7.0334214E-4	45.0	29