##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1204477
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13034038840094	33.0	31.0	34.0	30.0	34.0
2	32.30159313959503	34.0	31.0	34.0	30.0	34.0
3	32.304961406485965	34.0	31.0	34.0	30.0	34.0
4	35.87200419767252	37.0	35.0	37.0	35.0	37.0
5	35.76587099629133	37.0	35.0	37.0	35.0	37.0
6	35.68909742568766	37.0	35.0	37.0	33.0	37.0
7	36.008758988341	37.0	35.0	37.0	35.0	37.0
8	35.81862667365172	37.0	35.0	37.0	35.0	37.0
9	37.42433188844619	39.0	37.0	39.0	35.0	39.0
10	37.266766405668186	39.0	37.0	39.0	34.0	39.0
11	37.16789693784107	39.0	37.0	39.0	34.0	39.0
12	37.05118321063831	39.0	37.0	39.0	33.0	39.0
13	36.95561143965389	39.0	37.0	39.0	33.0	39.0
14	38.05264940717008	40.0	37.0	41.0	33.0	41.0
15	38.15419140423603	40.0	37.0	41.0	33.0	41.0
16	38.24057412470309	40.0	37.0	41.0	34.0	41.0
17	38.227940425595506	40.0	37.0	41.0	34.0	41.0
18	38.22146126493075	40.0	37.0	41.0	34.0	41.0
19	38.19029504091817	40.0	37.0	41.0	34.0	41.0
20	38.13198425540712	40.0	36.0	41.0	34.0	41.0
21	38.05297735033545	40.0	36.0	41.0	34.0	41.0
22	38.00268000136159	40.0	36.0	41.0	34.0	41.0
23	37.80681075686792	40.0	36.0	41.0	33.0	41.0
24	37.54161349697836	39.0	35.0	41.0	33.0	41.0
25	37.4654617730351	39.0	35.0	41.0	33.0	41.0
26	37.620610439219675	39.0	36.0	41.0	33.0	41.0
27	37.711400881876536	39.0	36.0	41.0	33.0	41.0
28	37.692652495647486	40.0	36.0	41.0	33.0	41.0
29	37.71644705544398	40.0	36.0	41.0	33.0	41.0
30	37.616419408589785	40.0	36.0	41.0	33.0	41.0
31	37.53247509084856	40.0	36.0	41.0	33.0	41.0
32	37.45169397174043	40.0	36.0	41.0	33.0	41.0
33	37.35220846890393	40.0	35.0	41.0	33.0	41.0
34	37.297133942781805	40.0	35.0	41.0	33.0	41.0
35	37.227554365919815	40.0	35.0	41.0	32.0	41.0
36	37.16961054465963	40.0	35.0	41.0	32.0	41.0
37	37.10702238398907	40.0	35.0	41.0	32.0	41.0
38	37.02700425163785	39.0	35.0	41.0	32.0	41.0
39	37.05191713914006	39.0	35.0	41.0	32.0	41.0
40	36.936428009833314	39.0	35.0	41.0	31.0	41.0
41	36.8451228209422	39.0	35.0	41.0	31.0	41.0
42	36.76428192485203	39.0	35.0	41.0	31.0	41.0
43	36.71610001685379	39.0	35.0	41.0	31.0	41.0
44	36.65910183424009	39.0	35.0	41.0	31.0	41.0
45	36.63526078123534	39.0	35.0	41.0	31.0	41.0
46	36.570765568790435	39.0	35.0	41.0	31.0	41.0
47	36.44909533349329	39.0	35.0	41.0	31.0	41.0
48	36.42391677051533	39.0	35.0	41.0	31.0	41.0
49	36.41083225333485	39.0	35.0	40.0	31.0	41.0
50	36.30580907730077	39.0	35.0	40.0	31.0	41.0
51	35.37652192611399	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	17.0
10	18.0
11	30.0
12	24.0
13	23.0
14	36.0
15	45.0
16	96.0
17	176.0
18	296.0
19	516.0
20	850.0
21	1314.0
22	2069.0
23	3126.0
24	4440.0
25	6605.0
26	9015.0
27	11014.0
28	12434.0
29	14314.0
30	17361.0
31	22044.0
32	28596.0
33	40575.0
34	74386.0
35	100251.0
36	88744.0
37	135851.0
38	253545.0
39	376405.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17770617454713	21.78829483668015	24.272858676421386	12.761140312351335
2	32.460561720979314	24.350568753077063	24.373234192101634	18.81563533384199
3	31.359004779667853	24.286806638898046	27.130862606757955	17.223325974676147
4	29.74818116078597	25.892648842609695	25.73017168447384	18.62899831213049
5	25.215757544560834	29.008274960833624	25.494799817680203	20.28116767692534
6	25.69521875469602	33.64422898901349	25.179725308162798	15.480826948127694
7	76.69710588080967	6.86007287810394	10.610580359774408	5.8322408813119715
8	76.28323330374927	5.351119199453373	10.685384610914115	7.68026288588325
9	69.1798182945793	8.364626306687468	13.968386278857961	8.487169119875265
10	38.33373323027339	26.731104039346537	20.898531063689884	14.036631666690191
11	30.37202038727182	25.19267698760541	25.334398249198614	19.10090437592416
12	29.162864878283273	21.298870796204493	30.129093374136655	19.409170951375575
13	24.55298025616097	25.028788428504654	30.22697818223179	20.19125313310258
14	17.59776234830553	29.861093237977975	29.422313585066384	23.11883082865011
15	17.20580799799415	27.28869044406825	35.88910373548021	19.616397822457383
16	19.1061348618529	25.532575549387825	33.98180289038313	21.37948669837614
17	17.91134243327187	26.076712133149904	29.717462433902845	26.294482999675377
18	19.955466148378093	27.56466084449931	30.86625979574537	21.61361321137722
19	22.657966901817137	28.408097456406388	27.546561702714122	21.38737393906235
20	25.427965830812877	25.717386052203572	28.830604486428552	20.024043630555006
21	22.969803491473893	26.98590342530409	29.04281277268059	21.00148031054142
22	20.29262493181688	26.430890751753665	26.98880925082007	26.28767506560939
23	20.971176701589155	28.03158549312274	28.018052648576937	22.97918515671117
24	22.726378336821707	24.228025939889264	29.373744787156586	23.671850936132447
25	20.679514843371855	26.168121101523734	27.50961620686821	25.64274784823621
26	19.278076708812204	28.935629322934354	27.864791108506015	23.921502859747427
27	20.95349267773482	28.16002298092865	28.97846949339838	21.90801484793815
28	18.071577954581116	27.037295025143692	30.586055192419614	24.305071827855578
29	19.217801585252353	24.885074600843353	29.37316362205339	26.523960191850904
30	20.43775016044308	23.99863177129991	30.966801358597962	24.59681670965905
31	19.864970439452144	26.534089069363713	28.98685487560161	24.614085615582532
32	22.109264020815676	26.138647728433174	26.865851319701413	24.88623693104974
33	21.39492908540387	25.235932276000288	29.24049193135278	24.12864670724306
34	19.16408532499998	23.757116159129644	31.826261522635967	25.252536993234408
35	20.860921379154604	22.000171028587513	28.982371601948397	28.15653599030949
36	20.25493222369543	24.442974004484935	29.908001564164366	25.394092207655273
37	20.05127536681896	24.89702999725192	32.734705602514616	22.3169890334145
38	20.364025215923593	24.888644615048687	28.319594313548535	26.427735855479185
39	21.316388772886487	22.24816248047908	30.04523955210436	26.390209194530073
40	22.660208538643744	21.286334234692735	33.43334908014018	22.620108146523346
41	19.500579919749402	23.95114228001033	30.018007815840402	26.53026998439987
42	21.47122776109465	23.138839512917226	28.76111374480376	26.628818981184367
43	22.17153171044362	22.67075253408741	29.6213211211173	25.536394634351673
44	20.221058600537827	23.782355329325508	29.485743604900716	26.51084246523595
45	20.535634968538215	21.47421661019679	28.617815035073313	29.372333386191684
46	21.080103646644975	23.169475216214174	30.08002643470984	25.670394702431015
47	19.24212749600034	22.299471056732507	33.56602077084079	24.892380676426367
48	19.592653076812592	20.28855677609452	32.10322820610107	28.015561940991816
49	19.84612408539142	20.31653572463401	33.7627866700651	26.074553519909472
50	19.007668888654578	20.07493708887758	32.81432522165222	28.103068800815624
51	18.15460154075171	20.922856974437867	28.987436040704807	31.935105444105616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	800.0
1	1134.5
2	1469.0
3	4869.0
4	8269.0
5	5683.0
6	3097.0
7	3037.5
8	2978.0
9	2961.5
10	2945.0
11	2865.5
12	2786.0
13	2810.0
14	2834.0
15	2689.5
16	2545.0
17	2677.0
18	2809.0
19	2817.0
20	2825.0
21	3017.5
22	3210.0
23	4003.0
24	4796.0
25	5771.5
26	8324.0
27	9901.0
28	12087.0
29	14273.0
30	14934.5
31	15596.0
32	19230.0
33	22864.0
34	24460.5
35	26057.0
36	28089.0
37	30121.0
38	34133.5
39	38146.0
40	44542.0
41	50938.0
42	57799.0
43	64660.0
44	75179.0
45	85698.0
46	100912.0
47	116126.0
48	134380.0
49	152634.0
50	145487.5
51	138341.0
52	114372.0
53	90403.0
54	76847.0
55	63291.0
56	56018.0
57	48745.0
58	47699.5
59	46654.0
60	46168.5
61	45683.0
62	41728.5
63	37774.0
64	31160.0
65	24546.0
66	19447.0
67	14348.0
68	10911.5
69	7475.0
70	6348.0
71	5221.0
72	4173.5
73	3126.0
74	2458.5
75	1327.5
76	864.0
77	681.5
78	499.0
79	390.0
80	281.0
81	186.0
82	91.0
83	91.0
84	91.0
85	78.5
86	66.0
87	56.0
88	46.0
89	27.0
90	8.0
91	6.5
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1204477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.08567339118782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.16603293770387	15.274874259904076
2	12.521859455360667	5.781511152736267
3	5.944420574138562	4.116928556232601
4	3.358463768983355	3.1012959066754964
5	2.1529387771963266	2.485102072078884
6	1.4622680202350717	2.0254465155315415
7	1.0103009962522351	1.632643517838954
8	0.7748712301661899	1.4310739311875667
9	0.5751045523903912	1.1949008276042852
>10	4.0949649210830765	20.705218210102995
>50	1.5520366530427467	25.74221750403865
>100	0.37330214143116897	12.055211660666608
>500	0.009078359470596207	1.3629409269622064
>1k	0.0036313437882384827	1.9061723949604286
>5k	7.262687576476966E-4	1.1844625634794572
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	7843	0.6511539863359781	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	6286	0.5218862626683615	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4667	0.3874710766581678	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	4551	0.37784034066237876	TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2798	0.23229999410532537	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2196	0.18231979523062708	TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1903	0.15799388448264268	TruSeq Adapter, Index 27 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1544	0.128188417047399	TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1426	0.11839163387926877	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1290	0.10710042616006782	TruSeq Adapter, Index 27 (95% over 22bp)
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1242	0.10311529402387924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.660471723411904E-4	0.0	0.0	0.39560738810288615	0.0
2	1.660471723411904E-4	0.0	0.0	2.075174536334027	0.0
3	1.660471723411904E-4	0.0	0.0	2.971248101873261	0.0
4	1.660471723411904E-4	0.0	0.0	3.8920626960913327	0.0
5	1.660471723411904E-4	0.0	0.0	6.3040639215194645	0.0
6	1.660471723411904E-4	0.0	0.0	7.522518072159119	0.0
7	1.660471723411904E-4	0.0	0.0	8.79053730374262	0.0
8	1.660471723411904E-4	0.0	0.0	10.797383428658248	0.0
9	1.660471723411904E-4	0.0	0.0	11.466636556779415	0.0
10	1.660471723411904E-4	0.0	0.0	12.962721579573541	0.0
11	1.660471723411904E-4	0.0	0.0	15.198297684389159	0.0
12	1.660471723411904E-4	0.0	0.0	16.919376625705596	0.0
13	1.660471723411904E-4	0.0	0.0	17.619348480709885	0.0
14	1.660471723411904E-4	0.0	0.0	17.902791003896297	0.0
15	1.660471723411904E-4	0.0	0.0	18.4290775166317	0.0
16	1.660471723411904E-4	0.0	0.0	19.521086745533538	0.0
17	1.660471723411904E-4	0.0	0.0	20.81542445393312	0.0
18	1.660471723411904E-4	0.0	0.0	22.321804401412397	0.0
19	1.660471723411904E-4	0.0	0.0	23.12148758340757	0.0
20	3.320943446823808E-4	0.0	0.0	23.908053038787788	0.0
21	3.320943446823808E-4	0.0	0.0	24.901845365249812	0.0
22	3.320943446823808E-4	0.0	0.0	25.921291979838553	0.0
23	3.320943446823808E-4	0.0	0.0	26.91408802326653	0.0
24	3.320943446823808E-4	0.0	0.0	27.66719497342	0.0
25	3.320943446823808E-4	0.0	0.0	28.32299828058153	0.0
26	3.320943446823808E-4	0.0	0.0	28.91728111039065	0.0
27	3.320943446823808E-4	0.0	0.0	29.510899751510408	0.0
28	3.320943446823808E-4	0.0	0.0	30.152423001850597	0.0
29	3.320943446823808E-4	0.0	0.0	30.786640176607772	0.0
30	3.320943446823808E-4	0.0	0.0	31.477230366374783	0.0
31	3.320943446823808E-4	0.0	0.0	32.16557891931519	0.0
32	3.320943446823808E-4	0.0	0.0	32.803117037519186	0.0
33	3.320943446823808E-4	0.0	0.0	33.39266752291658	0.0
34	3.320943446823808E-4	0.0	0.0	34.01600860788541	0.0
35	3.320943446823808E-4	0.0	0.0	34.65935837712136	0.0
36	3.320943446823808E-4	0.0	0.0	35.331185236413816	0.0
37	3.320943446823808E-4	0.0	0.0	35.991305770056215	0.0
38	3.320943446823808E-4	0.0	0.0	36.5791957837302	0.0
39	3.320943446823808E-4	0.0	0.0	37.141929650794495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.1655887E-6	45.000004	1
TTAGCCG	25	3.8909166E-5	45.000004	1
TACGGAC	30	2.1655887E-6	45.000004	14
CGCCTAC	30	2.1655887E-6	45.000004	29
TACGCGC	35	1.2119926E-7	45.000004	34
CGTAGGT	25	3.8909166E-5	45.000004	28
CGTACTA	35	1.2119926E-7	45.000004	18
GTTCACG	25	3.8909166E-5	45.000004	1
GGACCGT	25	3.8909166E-5	45.000004	8
GCGTAAG	65	0.0	45.000004	1
CCGCAAT	25	3.8909166E-5	45.000004	43
GTACGTC	45	3.8562575E-10	45.000004	1
TACGTAG	35	1.2119926E-7	45.000004	1
CTCGTCG	20	7.0334214E-4	45.0	31
ATTAGCG	20	7.0334214E-4	45.0	1
CGTATCA	20	7.0334214E-4	45.0	18
CTATCGT	20	7.0334214E-4	45.0	44
CGTGCCA	20	7.0334214E-4	45.0	21
CGACCTA	20	7.0334214E-4	45.0	38
CCTACGC	20	7.0334214E-4	45.0	29
>>END_MODULE