FastQCFastQC Report
Sat 18 Jun 2016
SRR3549837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646381
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC124380.7554751907365307No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC79050.4801440249857111No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT65610.3985104298458255No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54880.3333371801545329No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC49330.2996268785900712No Hit
ACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC35670.21665701924402672No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG26960.16375310453655625No Hit
AGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG23540.14298027005899605No Hit
GCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG22710.13793890964485134No Hit
ACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG21710.13186498143503844No Hit
GGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG18880.11467576460126787No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC17100.10386417238780088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG351.2123564E-745.0000041
AGTATCG351.2123564E-745.00000431
TACCCGT253.891544E-545.036
TACCCGA207.0341764E-445.019
GTCGATA207.0341764E-445.03
CGTCTTA207.0341764E-445.039
TAACGGC207.0341764E-445.013
TAGCCGT207.0341764E-445.044
CCGTAAC207.0341764E-445.022
CGTAGAA207.0341764E-445.024
AACGACG207.0341764E-445.01
TCGTAGC207.0341764E-445.018
CAGTCGT207.0341764E-445.039
ACTCCGC207.0341764E-445.042
CGCAATA253.891544E-545.020
CGAAGTC253.891544E-545.026
TTAGCCG302.1660762E-644.9999961
CGCGAAT302.1660762E-644.99999637
CGTTTTT36050.042.565881
ATTCCGG556.184564E-1140.9090922