##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1646381
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970545699932153	33.0	31.0	34.0	30.0	34.0
2	32.14457346142843	33.0	31.0	34.0	30.0	34.0
3	32.15893465728771	34.0	31.0	34.0	30.0	34.0
4	35.75403870671491	37.0	35.0	37.0	35.0	37.0
5	35.63900761731337	37.0	35.0	37.0	33.0	37.0
6	35.56418714744643	37.0	35.0	37.0	33.0	37.0
7	35.963327443647614	37.0	35.0	37.0	35.0	37.0
8	35.82143562152382	37.0	35.0	37.0	35.0	37.0
9	37.553752138781974	39.0	37.0	39.0	35.0	39.0
10	37.15098327786824	39.0	37.0	39.0	34.0	39.0
11	37.04164224441366	39.0	37.0	39.0	33.0	39.0
12	36.991324000945106	39.0	37.0	39.0	33.0	39.0
13	36.90591545942282	39.0	37.0	39.0	33.0	39.0
14	37.92068239368652	40.0	37.0	41.0	33.0	41.0
15	37.993198415190655	40.0	37.0	41.0	33.0	41.0
16	38.114461354935464	40.0	37.0	41.0	33.0	41.0
17	38.122266960077894	40.0	37.0	41.0	34.0	41.0
18	38.106267018387605	40.0	37.0	41.0	34.0	41.0
19	38.07079770721358	40.0	37.0	41.0	34.0	41.0
20	38.02327104115026	40.0	37.0	41.0	34.0	41.0
21	37.96965647684224	40.0	36.0	41.0	34.0	41.0
22	37.92143434599889	40.0	36.0	41.0	33.0	41.0
23	37.730825367882645	39.0	36.0	41.0	33.0	41.0
24	37.45818009318621	39.0	36.0	41.0	32.0	41.0
25	37.40416222004506	39.0	35.0	41.0	32.0	41.0
26	37.61003072800281	39.0	36.0	41.0	33.0	41.0
27	37.74090444435401	39.0	36.0	41.0	33.0	41.0
28	37.73774417950644	39.0	36.0	41.0	33.0	41.0
29	37.75022549458479	40.0	36.0	41.0	33.0	41.0
30	37.63049561432014	39.0	36.0	41.0	33.0	41.0
31	37.510942485366385	39.0	36.0	41.0	33.0	41.0
32	37.430440463051994	39.0	36.0	41.0	33.0	41.0
33	37.32475654177253	39.0	36.0	41.0	33.0	41.0
34	37.24541463974621	39.0	36.0	41.0	32.0	41.0
35	37.20894009345346	39.0	36.0	41.0	32.0	41.0
36	37.15980079945043	39.0	35.0	41.0	32.0	41.0
37	37.06837785421479	39.0	35.0	41.0	32.0	41.0
38	37.013466506233975	39.0	35.0	41.0	31.0	41.0
39	36.99943998381905	39.0	35.0	41.0	32.0	41.0
40	36.90308318669858	39.0	35.0	41.0	31.0	41.0
41	36.86172702430361	39.0	35.0	41.0	31.0	41.0
42	36.817372770944274	39.0	35.0	41.0	31.0	41.0
43	36.785069798546026	39.0	35.0	41.0	31.0	41.0
44	36.73555574317245	39.0	35.0	41.0	31.0	41.0
45	36.682302577592914	39.0	35.0	41.0	31.0	41.0
46	36.606074778559766	39.0	35.0	40.0	31.0	41.0
47	36.47697404185301	39.0	35.0	40.0	31.0	41.0
48	36.47890919538066	39.0	35.0	40.0	31.0	41.0
49	36.5022349018848	39.0	35.0	40.0	31.0	41.0
50	36.39841081742318	39.0	35.0	40.0	31.0	41.0
51	35.4097101460719	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	18.0
11	22.0
12	18.0
13	20.0
14	29.0
15	42.0
16	83.0
17	151.0
18	283.0
19	568.0
20	934.0
21	1565.0
22	2497.0
23	3791.0
24	5826.0
25	8610.0
26	11906.0
27	14425.0
28	16174.0
29	19258.0
30	23980.0
31	31282.0
32	41449.0
33	59113.0
34	101080.0
35	128623.0
36	132315.0
37	207991.0
38	378635.0
39	455454.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.14946661799426	23.366158866021898	25.531878708512796	9.952495807471053
2	33.06178824949996	23.120893644909653	25.54366212923983	18.273655976350554
3	30.667385009909616	22.62756919570865	29.320309211537303	17.384736582844432
4	27.76884572890479	25.021061346067526	28.744500817247044	18.46559210778064
5	23.66068364491573	28.510958277579736	28.182662457839346	19.645695619665194
6	24.927279894508015	32.57982204605131	27.372886348907087	15.120011710533587
7	77.82281258104898	5.134048558626466	11.12658613042789	5.916552729896664
8	77.20588369277827	3.7605511725414718	11.163697831789847	7.8698673028904
9	72.12820118793888	5.686411590026853	14.085014343581467	8.1003728784528
10	37.90811482882759	22.023456295960656	23.412685156109067	16.655743719102688
11	26.18549412317076	24.374005773876156	27.254444748815736	22.186055354137345
12	24.516560868960465	20.906096462483468	33.40204970781369	21.17529296074238
13	22.787495725473022	24.492568852531704	34.25574031770289	18.464195104292383
14	19.512798070434485	27.137400152212642	31.84378342558618	21.50601835176669
15	17.092216200259845	26.200132290156407	36.45857186155574	20.249079648028008
16	19.392716509726483	26.279943706833354	32.2196988424915	22.107640940948663
17	19.92697923506163	26.216896332015494	28.206107820729226	25.650016612193653
18	20.893037516832376	25.43682173202922	33.037796233071205	20.632344518067203
19	19.951092730054587	28.81009924191302	28.934979205906775	22.30382882212562
20	20.281696642514703	28.42349371135843	30.284606054127206	21.010203591999666
21	20.187004101723723	27.829888707413414	31.86813987770753	20.114967313155336
22	18.493410698981585	25.071353471644777	29.16433073510931	27.27090509426433
23	17.30923765519646	26.5898962633801	30.692531072698237	25.408335008725196
24	20.79251400495997	24.65043024670474	30.62371346608106	23.93334228225423
25	18.32643841249383	27.30504057080348	28.557909742641584	25.810611274061106
26	17.02807551836422	26.6840421506322	32.27679376766374	24.011088563339836
27	20.042444610330172	25.574639163109875	30.1126531465074	24.27026308005255
28	15.594081807309488	24.757756558172137	31.514212080921734	28.13394955359665
29	17.213876982302395	22.43982407474333	33.86020611267987	26.486092830274398
30	21.05290330731465	24.58009415803511	29.125032419591818	25.24197011505842
31	18.51205765858571	27.348226200375247	26.71483696665596	27.424879174383086
32	18.487276031489674	25.89273078345778	29.436867893883615	26.183125291168935
33	20.567657182632697	23.93789772841159	28.09137131684586	27.40307377210986
34	17.01993645456307	24.678309577187786	30.902081595936785	27.399672372312363
35	17.02667851487596	22.460110994964104	31.41514631181968	29.09806417834025
36	19.59698271542249	23.503308164999474	30.24804100630413	26.651668113273903
37	16.606909336295793	25.110165872905483	31.714105058306675	26.568819732492056
38	18.056027128592955	24.633848422691955	29.45776220692537	27.85236224178972
39	21.36965866345639	23.384380650651337	31.765551230243787	23.48040945564848
40	21.141947094870506	23.56380448996921	30.466641682575297	24.827606732584982
41	16.96861176119015	23.942878349543637	29.102862581626006	29.98564730764021
42	17.887597099334844	21.23998029617689	32.812392757205046	28.060029847283225
43	19.414825608410204	20.95159018477497	32.20609324330152	27.427490963513307
44	19.148605334974103	21.61911489503341	29.701144510292576	29.53113525969991
45	20.397344235629543	21.20456929471368	29.101890753112432	29.29619571654435
46	22.393601481066653	22.199357256916837	29.290182527616633	26.116858734399873
47	16.147416667223442	22.80146576035559	33.631279758452024	27.41983781396894
48	16.7169689154576	20.539413416457066	32.64371977081854	30.099897897266793
49	19.740388160456178	18.648599564742305	34.74614928136318	26.864862993438337
50	19.163243501959755	18.618594359385828	33.074543498740574	29.14361863991385
51	18.604685063785357	18.817394029693006	28.69317612387412	33.884744782647516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	801.0
1	1133.0
2	1465.0
3	5444.0
4	9423.0
5	6476.0
6	3529.0
7	3514.5
8	3500.0
9	3594.0
10	3688.0
11	3706.5
12	3725.0
13	3747.0
14	3769.0
15	3775.0
16	3781.0
17	3883.5
18	3986.0
19	3934.0
20	3882.0
21	4099.0
22	4316.0
23	4915.0
24	5514.0
25	6147.0
26	8152.5
27	9525.0
28	11722.0
29	13919.0
30	15263.5
31	16608.0
32	19450.0
33	22292.0
34	26894.0
35	31496.0
36	36571.0
37	41646.0
38	46063.5
39	50481.0
40	58429.0
41	66377.0
42	78654.0
43	90931.0
44	110878.0
45	130825.0
46	158309.0
47	185793.0
48	218403.5
49	251014.0
50	241327.5
51	231641.0
52	186460.0
53	141279.0
54	115619.5
55	89960.0
56	75892.5
57	61825.0
58	53704.0
59	45583.0
60	39644.5
61	33706.0
62	30088.5
63	26471.0
64	21113.5
65	15756.0
66	13355.5
67	10955.0
68	9790.5
69	8626.0
70	6950.5
71	5275.0
72	4320.0
73	3365.0
74	2513.0
75	1121.0
76	581.0
77	455.5
78	330.0
79	256.0
80	182.0
81	133.0
82	84.0
83	53.0
84	22.0
85	13.0
86	4.0
87	5.0
88	6.0
89	4.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1646381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.47149593861768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.95043489346853	18.777075392194586
2	13.757958027703038	7.83419292218839
3	6.258006681285789	5.345244354302118
4	3.4496132892998417	3.9286260302440805
5	2.0744130125514424	2.9530820830937032
6	1.4020547553613605	2.395115776379434
7	1.0100346059323555	2.013003732646646
8	0.7339597309692095	1.671754519951902
9	0.5665122409277293	1.451650587007594
>10	3.7237806428792126	21.472814767747774
>50	0.8580853087623735	17.400232768514453
>100	0.20019704219139825	8.804754027429025
>500	0.010616502387217567	2.0417875009296047
>1k	0.003466613024397573	1.912490948104793
>5k	6.499899420745449E-4	1.2309081178751422
>10k+	2.166633140248483E-4	0.7672664713907497
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	12438	0.7554751907365307	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	7905	0.4801440249857111	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	6561	0.3985104298458255	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5488	0.3333371801545329	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	4933	0.2996268785900712	No Hit
ACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	3567	0.21665701924402672	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	2696	0.16375310453655625	No Hit
AGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	2354	0.14298027005899605	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	2271	0.13793890964485134	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	2171	0.13186498143503844	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	1888	0.11467576460126787	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	1710	0.10386417238780088	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2147856419625833E-4	0.0	0.0	0.4202550928369557	0.0
2	1.2147856419625833E-4	0.0	0.0	2.275111289549624	0.0
3	1.2147856419625833E-4	0.0	0.0	3.310473092194334	0.0
4	1.2147856419625833E-4	0.0	0.0	4.503939246140474	0.0
5	1.2147856419625833E-4	0.0	0.0	7.568418245837385	0.0
6	1.2147856419625833E-4	0.0	0.0	9.218400844033065	0.0
7	1.2147856419625833E-4	0.0	0.0	10.798897703508484	0.0
8	1.2147856419625833E-4	0.0	0.0	13.381106803346249	0.0
9	1.2147856419625833E-4	0.0	0.0	14.206310689931431	0.0
10	1.2147856419625833E-4	0.0	0.0	16.45117381699619	0.0
11	1.2147856419625833E-4	0.0	0.0	19.719372368850223	0.0
12	1.2147856419625833E-4	0.0	0.0	21.918012902238303	0.0
13	1.2147856419625833E-4	0.0	0.0	22.811123306209193	0.0
14	1.2147856419625833E-4	0.0	0.0	23.12362691260407	0.0
15	1.2147856419625833E-4	0.0	0.0	23.76703812786955	0.0
16	1.2147856419625833E-4	0.0	0.0	25.332471645384633	0.0
17	1.2147856419625833E-4	0.0	0.0	27.289187618175866	0.0
18	1.2147856419625833E-4	0.0	0.0	29.39677996769885	0.0
19	1.2147856419625833E-4	0.0	0.0	30.662465128059665	0.0
20	1.2147856419625833E-4	0.0	0.0	31.77405472973753	0.0
21	1.822178462943875E-4	0.0	0.0	33.30796455984368	0.0
22	1.822178462943875E-4	0.0	0.0	34.87916830915809	0.0
23	1.822178462943875E-4	0.0	0.0	36.52945460376426	0.0
24	1.822178462943875E-4	0.0	0.0	37.70797889431426	0.0
25	1.822178462943875E-4	0.0	0.0	38.76678605984885	0.0
26	2.4295712839251667E-4	0.0	0.0	39.73175103454182	0.0
27	2.4295712839251667E-4	0.0	0.0	40.67466764983318	0.0
28	2.4295712839251667E-4	0.0	0.0	41.663138726698136	0.0
29	2.4295712839251667E-4	0.0	0.0	42.67681660563381	0.0
30	2.4295712839251667E-4	0.0	0.0	43.76404975519032	0.0
31	2.4295712839251667E-4	0.0	0.0	44.797103465115306	0.0
32	2.4295712839251667E-4	0.0	0.0	45.719186506647006	0.0
33	2.4295712839251667E-4	0.0	0.0	46.61332947841356	0.0
34	3.0369641049064587E-4	0.0	0.0	47.49441350452903	0.0
35	3.0369641049064587E-4	0.0	0.0	48.369302123870476	0.0
36	3.0369641049064587E-4	0.0	0.0	49.245405528853894	0.0
37	4.2517497468690416E-4	0.0	0.0	50.12393850512123	0.0
38	4.2517497468690416E-4	0.0	0.0	50.96554199787291	0.0
39	4.2517497468690416E-4	0.0	0.0	51.919634641070324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	35	1.2123564E-7	45.000004	1
AGTATCG	35	1.2123564E-7	45.000004	31
TACCCGT	25	3.891544E-5	45.0	36
TACCCGA	20	7.0341764E-4	45.0	19
GTCGATA	20	7.0341764E-4	45.0	3
CGTCTTA	20	7.0341764E-4	45.0	39
TAACGGC	20	7.0341764E-4	45.0	13
TAGCCGT	20	7.0341764E-4	45.0	44
CCGTAAC	20	7.0341764E-4	45.0	22
CGTAGAA	20	7.0341764E-4	45.0	24
AACGACG	20	7.0341764E-4	45.0	1
TCGTAGC	20	7.0341764E-4	45.0	18
CAGTCGT	20	7.0341764E-4	45.0	39
ACTCCGC	20	7.0341764E-4	45.0	42
CGCAATA	25	3.891544E-5	45.0	20
CGAAGTC	25	3.891544E-5	45.0	26
TTAGCCG	30	2.1660762E-6	44.999996	1
CGCGAAT	30	2.1660762E-6	44.999996	37
CGTTTTT	3605	0.0	42.56588	1
ATTCCGG	55	6.184564E-11	40.909092	2
>>END_MODULE