FastQCFastQC Report
Sat 18 Jun 2016
SRR3549833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895161
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC127110.6707081878531692No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC93500.49336177770648515No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT72020.38002048374781877TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70920.37421622753950723No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC56310.2971251519000233No Hit
CGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG42160.22246131067492417No Hit
ACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC37290.1967642854617629TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG27670.14600342662180155No Hit
AGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG24190.12764087061732488No Hit
GCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG23840.12579406182377117No Hit
GGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG20470.10801193144012566No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC20180.10648171843975261No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC20020.10563746299127094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.034446E-445.00000426
GCGCGAA253.8917668E-545.00000417
CCGTGAT207.034446E-445.00000433
CCTACGG207.034446E-445.0000042
CACGAAA253.8917668E-545.00000442
TCGGCTA207.034446E-445.00000439
CGTTAGA207.034446E-445.00000422
CCCGTAG207.034446E-445.00000414
AACGCGC207.034446E-445.00000440
GTCGTAC207.034446E-445.0000048
TCCGTCA253.8917668E-545.00000429
CGTCGTC253.8917668E-545.00000434
ACCGTCG207.034446E-445.00000419
CGCAATT253.8917668E-545.00000431
CGACTCG207.034446E-445.0000042
CGCAACT207.034446E-445.00000442
TGTACCG207.034446E-445.0000042
TATCGCA253.8917668E-545.00000428
ATACCGG502.1827873E-1145.0000042
GCGTAAT207.034446E-445.0000049