FastQCFastQC Report
Sat 18 Jun 2016
SRR3549832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3457074
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC212630.6150577048683367No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC204710.5921481576616526No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG179420.518993808058491No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157310.4550379887731648No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC115910.3352835374654983TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC72410.2094545850045443TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG61810.17879281727842677No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT59170.17115630154286543TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT43490.12580002626498593No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT41090.11885773923265744No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC39220.11344854058663482No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT38310.11081625675354359No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC35330.10219625035506905TruSeq Adapter, Index 13 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA207.035254E-445.0000042
TCGAACG207.035254E-445.0000041
CGTTTTT110300.042.6745221
CGCGAAT356.251932E-638.57142635
TTACGCG700.038.5714261
CGCGTAA356.251932E-638.57142631
GTACGAC356.251932E-638.57142623
CGACGGT1200.037.50000428
TACGTCG301.140396E-437.5000041
TACGGCT26400.036.9034087
AGGGCGA31300.036.589466
TAAGGGA30800.036.5259744
TAGTGCG2350.036.382981
CGAAACG250.002107692436.00000435
GCGCGTA250.002107692436.0000043
CGTTACC250.002107692436.00000436
TCTCGAC250.002107692436.00000417
CGCAACA250.002107692436.00000418
CGTAAGG4650.035.8064542
GCGATGT10000.035.3259
Produced by FastQC (version 0.11.5)