##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549832_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3457074
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.201961253939025	33.0	31.0	34.0	30.0	34.0
2	32.38393161384454	34.0	31.0	34.0	30.0	34.0
3	32.380960893518626	34.0	31.0	34.0	30.0	34.0
4	35.9369594055551	37.0	35.0	37.0	35.0	37.0
5	35.83207996126204	37.0	35.0	37.0	35.0	37.0
6	35.75806303249511	37.0	35.0	37.0	35.0	37.0
7	36.06654066415703	37.0	35.0	37.0	35.0	37.0
8	35.84996184634752	37.0	35.0	37.0	35.0	37.0
9	37.55648794327226	39.0	38.0	39.0	35.0	39.0
10	37.291267702108776	39.0	37.0	39.0	34.0	39.0
11	37.198042622171236	39.0	37.0	39.0	34.0	39.0
12	37.184555783301136	39.0	37.0	39.0	34.0	39.0
13	37.17589383391851	39.0	37.0	39.0	34.0	39.0
14	38.37494569106707	40.0	38.0	41.0	34.0	41.0
15	38.39744911448236	40.0	38.0	41.0	34.0	41.0
16	38.48684812647922	40.0	38.0	41.0	34.0	41.0
17	38.42934921265787	40.0	38.0	41.0	34.0	41.0
18	38.38706952758315	40.0	38.0	41.0	34.0	41.0
19	38.34710538449568	40.0	37.0	41.0	34.0	41.0
20	38.24908405200467	40.0	37.0	41.0	34.0	41.0
21	38.21567718828119	40.0	37.0	41.0	34.0	41.0
22	38.16932643038593	40.0	37.0	41.0	34.0	41.0
23	37.950889683009386	40.0	36.0	41.0	33.0	41.0
24	37.679757216652	40.0	36.0	41.0	33.0	41.0
25	37.61900641987993	39.0	36.0	41.0	33.0	41.0
26	37.793786016729754	40.0	36.0	41.0	33.0	41.0
27	37.821149908853556	40.0	36.0	41.0	33.0	41.0
28	37.758960612356	40.0	36.0	41.0	33.0	41.0
29	37.76418236925215	40.0	36.0	41.0	33.0	41.0
30	37.60892072313176	40.0	36.0	41.0	33.0	41.0
31	37.55095783312709	40.0	36.0	41.0	33.0	41.0
32	37.39015248154943	40.0	36.0	41.0	33.0	41.0
33	37.22267356729998	40.0	36.0	41.0	32.0	41.0
34	37.13540091996874	40.0	36.0	41.0	32.0	41.0
35	37.03589943403005	40.0	36.0	41.0	31.0	41.0
36	37.000528770862296	40.0	36.0	41.0	31.0	41.0
37	36.94054046861594	40.0	35.0	41.0	31.0	41.0
38	36.86800051141515	39.0	35.0	41.0	31.0	41.0
39	36.86646973712452	39.0	35.0	41.0	31.0	41.0
40	36.7910831529785	39.0	35.0	41.0	31.0	41.0
41	36.75777261348759	39.0	35.0	41.0	31.0	41.0
42	36.68504376822712	39.0	35.0	41.0	31.0	41.0
43	36.63775233043898	39.0	35.0	41.0	31.0	41.0
44	36.59261502646458	39.0	35.0	41.0	31.0	41.0
45	36.55568032388083	39.0	35.0	41.0	30.0	41.0
46	36.491335447259736	39.0	35.0	41.0	30.0	41.0
47	36.36844684261893	39.0	35.0	41.0	30.0	41.0
48	36.32274692413295	39.0	35.0	41.0	30.0	41.0
49	36.32475613770489	39.0	35.0	41.0	30.0	41.0
50	36.23197276077978	39.0	35.0	41.0	30.0	41.0
51	35.33949895200392	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	35.0
10	48.0
11	33.0
12	37.0
13	42.0
14	61.0
15	85.0
16	177.0
17	319.0
18	649.0
19	1409.0
20	2481.0
21	4463.0
22	7036.0
23	10565.0
24	15266.0
25	22254.0
26	30834.0
27	36099.0
28	38767.0
29	41949.0
30	49799.0
31	62119.0
32	79714.0
33	108348.0
34	183369.0
35	245002.0
36	256545.0
37	397542.0
38	737390.0
39	1123804.0
40	820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.089603520202346	21.000157937029986	25.097032924374773	12.813205618392894
2	31.34078125027118	26.148500147812857	25.407671342875503	17.103047259040448
3	29.902773270112238	25.79704686680123	28.036426180058626	16.2637536830279
4	26.54768165217175	24.511046046454315	30.93396323017673	18.007309071197202
5	26.636109033246036	28.61885513587502	26.211617107415115	18.533418723463832
6	25.675412212755646	34.684765208959945	25.084739291088358	14.55508328719605
7	78.84135543526115	4.889134568713311	11.417834851090836	4.851675144934705
8	79.90740146146712	5.733345598040423	9.316491344992905	5.042761595499547
9	74.07258276797083	6.58224267111436	11.098692130975502	8.246482429939308
10	37.384071038109106	30.840936583943527	18.25821489502394	13.51677748292342
11	26.0527255129627	23.61219343294358	30.89586743008683	19.43921362400689
12	24.05054100664319	21.27865935181023	33.293096994741795	21.37770264680478
13	22.322895026256308	22.886435176105575	35.72226107974547	19.068408717892645
14	19.75450916005848	27.239480554943285	31.824658656424482	21.181351628573758
15	18.374787464775125	25.48959032985698	36.371046729112535	19.764575476255352
16	21.036315681990033	26.03105979218264	31.548644894497485	21.383979631329847
17	20.810344239087737	25.56358353914322	31.406241231746847	22.2198309900222
18	20.845692050560675	25.714780765468138	31.79156708823705	21.64796009573414
19	20.83721667514204	27.097424006544262	30.07204936891718	21.993309949396515
20	22.638421971875637	26.78166565135719	30.759856456645128	19.820055920122044
21	22.05460455865278	28.100266294560083	30.634114282772078	19.21101486401506
22	21.35713612147151	24.13919979728522	30.7958984968213	23.70776558442197
23	20.06401945691646	27.07801452904971	30.826704895527257	22.031261118506578
24	21.24244375445825	24.684603222262528	30.93636410444208	23.136588918837145
25	19.64872606140337	28.172755341655982	29.02333013409606	23.15518846284459
26	19.141013469772414	26.845823954014293	30.535794142676725	23.47736843353657
27	21.297490305385423	27.300746238003583	29.069322785685237	22.33244067092576
28	18.15491944922209	27.179950443641065	31.663134778138968	23.001995328997875
29	19.76052580881983	25.58235664032647	29.831383418463126	24.82573413239057
30	20.30500359552616	28.182127429149624	29.41632143251779	22.09654754280643
31	20.31026816319234	27.176392521537	28.63919025164055	23.87414906363011
32	20.407373403057036	29.361303807786587	26.873940216495225	23.35738257266116
33	19.894714431915546	28.67025698611022	27.134131349227697	24.30089723274654
34	19.2646440313398	26.519189349143236	28.53152695024752	25.684639669269444
35	18.110778074174867	27.763044701964724	27.215471812289817	26.910705411570596
36	18.56740700372627	28.384813284297643	27.277055683505765	25.770724028470323
37	18.88579764274644	28.690939216227363	28.884657950625297	23.5386051904009
38	18.361047521690306	28.686397803460384	27.62691802373915	25.325636651110155
39	20.068618722075374	27.518820829406604	27.057071963168855	25.35548848534917
40	19.83908935706901	25.913908698512095	28.51096042491425	25.736041519504642
41	17.952580708425682	26.286304545404583	27.163636069115093	28.597478677054646
42	19.508520789546306	25.29425172848484	28.53936594935486	26.657861532613996
43	20.285623044227574	25.819175406716777	28.258376881721365	25.636824667334285
44	19.68398709428841	26.441464660577125	28.50124122306899	25.373307022065482
45	19.780050991098253	25.069176997657554	27.988611178123463	27.162160833120723
46	21.392831047296067	26.73998300296725	27.004310581723157	24.86287536801353
47	17.75981653849469	25.71729734451736	30.872466137548688	25.65041997943926
48	18.71394711250034	24.893681766719485	29.430639899521964	26.961731221258205
49	19.505078572226108	23.81511069765935	31.21402665953925	25.465784070575292
50	18.828552700925698	23.519600679649898	30.463449726560672	27.188396892863736
51	18.059434076331605	23.45006210454274	28.700774122856497	29.789729696269156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	441.0
1	1232.5
2	2024.0
3	15320.0
4	28616.0
5	19485.0
6	10354.0
7	10085.5
8	9817.0
9	9736.5
10	9656.0
11	9762.5
12	9869.0
13	9697.0
14	9525.0
15	9181.0
16	8837.0
17	8768.5
18	8700.0
19	8916.5
20	9133.0
21	10106.0
22	11079.0
23	13174.5
24	15270.0
25	18739.5
26	27412.0
27	32615.0
28	38657.0
29	44699.0
30	53058.5
31	61418.0
32	70447.0
33	79476.0
34	90563.0
35	101650.0
36	108636.5
37	115623.0
38	127185.5
39	138748.0
40	152504.0
41	166260.0
42	180971.0
43	195682.0
44	220848.5
45	246015.0
46	319232.5
47	392450.0
48	385385.5
49	378321.0
50	362112.5
51	345904.0
52	297900.0
53	249896.0
54	214031.5
55	178167.0
56	158717.5
57	139268.0
58	130475.5
59	121683.0
60	112238.5
61	102794.0
62	92895.5
63	82997.0
64	68133.0
65	53269.0
66	42182.5
67	31096.0
68	24327.5
69	17559.0
70	13879.5
71	10200.0
72	7935.0
73	5670.0
74	4913.0
75	3708.0
76	3260.0
77	2376.5
78	1493.0
79	1097.5
80	702.0
81	492.5
82	283.0
83	166.5
84	50.0
85	69.0
86	88.0
87	59.5
88	31.0
89	19.5
90	8.0
91	8.5
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3457074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.24526181778539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79572520652003	16.224199061877613
2	11.97354622170601	5.566564336218225
3	5.026296728454319	3.505127502803964
4	2.73129613141502	2.5395877470658608
5	1.5996225760007623	1.8591822794389015
6	1.0612061771451882	1.4800809258234622
7	0.7642313084031891	1.2435329797226575
8	0.5296207127247212	0.9848937705126596
9	0.4163739482426605	0.8710849296905099
>10	4.145044908654933	23.036882946261912
>50	1.5959293660889848	26.327585392520053
>100	0.35205810527674636	10.772675480837894
>500	0.005225253296714241	0.8528436640692815
>1k	0.0028037944518954187	1.5855284459145347
>5k	3.82335607076648E-4	0.572917313444342
>10k+	6.372260117944134E-4	2.57731322379807
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	21263	0.6150577048683367	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC	20471	0.5921481576616526	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG	17942	0.518993808058491	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15731	0.4550379887731648	No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	11591	0.3352835374654983	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	7241	0.2094545850045443	TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTG	6181	0.17879281727842677	No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT	5917	0.17115630154286543	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT	4349	0.12580002626498593	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT	4109	0.11885773923265744	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	3922	0.11344854058663482	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT	3831	0.11081625675354359	No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	3533	0.10219625035506905	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7355717580821238E-4	0.0	0.0	0.18423094212041743	0.0
2	1.7355717580821238E-4	0.0	0.0	0.9589323225363414	0.0
3	1.7355717580821238E-4	0.0	0.0	1.487356070480412	0.0
4	1.7355717580821238E-4	0.0	0.0	2.1586752265065776	0.0
5	1.7355717580821238E-4	0.0	0.0	4.159529127811554	0.0
6	2.0248337177624777E-4	0.0	0.0	5.258840279380771	0.0
7	2.0248337177624777E-4	0.0	0.0	6.357543980834659	0.0
8	2.0248337177624777E-4	0.0	0.0	7.964943764582419	0.0
9	2.3140956774428317E-4	0.0	0.0	8.61011363945348	0.0
10	2.3140956774428317E-4	0.0	0.0	10.323759340991833	0.0
11	2.3140956774428317E-4	0.0	0.0	11.909869444507118	0.0
12	2.3140956774428317E-4	0.0	0.0	13.812663541480454	0.0
13	2.3140956774428317E-4	0.0	0.0	14.36929033049336	0.0
14	2.3140956774428317E-4	0.0	0.0	14.60911742126434	0.0
15	2.3140956774428317E-4	0.0	0.0	15.048824526174448	0.0
16	2.3140956774428317E-4	0.0	0.0	15.792401319728764	0.0
17	2.3140956774428317E-4	0.0	0.0	16.756916398086936	0.0
18	2.3140956774428317E-4	0.0	0.0	17.774800308006135	0.0
19	2.3140956774428317E-4	0.0	0.0	18.56671277502304	0.0
20	2.3140956774428317E-4	0.0	0.0	19.20100640021012	0.0
21	2.3140956774428317E-4	0.0	0.0	19.96749274097112	0.0
22	2.3140956774428317E-4	0.0	0.0	20.790327311477856	0.0
23	2.3140956774428317E-4	0.0	0.0	21.59595079538361	0.0
24	2.603357637123186E-4	0.0	0.0	22.24146778460629	0.0
25	2.603357637123186E-4	0.0	0.0	22.85178159333587	0.0
26	2.603357637123186E-4	0.0	0.0	23.379916079320257	0.0
27	2.603357637123186E-4	0.0	0.0	23.910885332509515	0.0
28	2.603357637123186E-4	0.0	0.0	24.44986714198192	0.0
29	2.603357637123186E-4	0.0	0.0	25.02040743125545	0.0
30	2.89261959680354E-4	0.0	0.0	25.657275487883684	0.0
31	3.4711435161642476E-4	0.0	0.0	26.258911437822853	0.0
32	3.7604054758446015E-4	0.0	0.0	26.82372434029471	0.0
33	3.7604054758446015E-4	0.0	0.0	27.373987366194648	0.0
34	4.0496674355249555E-4	0.0	0.0	27.927634757022847	0.0
35	4.0496674355249555E-4	0.0	0.0	28.499765987074618	0.0
36	4.0496674355249555E-4	0.0	0.0	29.05922754329239	0.0
37	4.0496674355249555E-4	0.0	0.0	29.611197214754444	0.0
38	4.3389293952053094E-4	0.0	0.0	30.162761919472942	0.0
39	4.3389293952053094E-4	0.0	0.0	30.71348776450837	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGA	20	7.035254E-4	45.000004	2
TCGAACG	20	7.035254E-4	45.000004	1
CGTTTTT	11030	0.0	42.674522	1
CGCGAAT	35	6.251932E-6	38.571426	35
TTACGCG	70	0.0	38.571426	1
CGCGTAA	35	6.251932E-6	38.571426	31
GTACGAC	35	6.251932E-6	38.571426	23
CGACGGT	120	0.0	37.500004	28
TACGTCG	30	1.140396E-4	37.500004	1
TACGGCT	2640	0.0	36.903408	7
AGGGCGA	3130	0.0	36.58946	6
TAAGGGA	3080	0.0	36.525974	4
TAGTGCG	235	0.0	36.38298	1
CGAAACG	25	0.0021076924	36.000004	35
GCGCGTA	25	0.0021076924	36.000004	3
CGTTACC	25	0.0021076924	36.000004	36
TCTCGAC	25	0.0021076924	36.000004	17
CGCAACA	25	0.0021076924	36.000004	18
CGTAAGG	465	0.0	35.806454	2
GCGATGT	1000	0.0	35.325	9
>>END_MODULE