SRR3549831_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1972514
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	6982	0.353964534598994	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	6428	0.3258785488974983	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5390	0.27325534825101366	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	4752	0.24091083764171004	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	4224	0.21414296679263112	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	4188	0.21231788468928484	TruSeq Adapter, Index 14 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	3727	0.18894669442143375	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	2160	0.10950492620077727	TruSeq Adapter, Index 15 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	35	1.2125201E-7	45.000004	1
CACTACG	30	2.1662963E-6	45.000004	18
ACGTGCG	30	2.1662963E-6	45.000004	1
TCGCAAT	25	3.891826E-5	45.0	30
CGAAACG	20	7.034517E-4	45.0	33
CGACGAA	20	7.034517E-4	45.0	19
TAACGCC	20	7.034517E-4	45.0	12
TATGCGA	20	7.034517E-4	45.0	14
TTATCCG	40	6.8193913E-9	45.0	1
CGTTATG	20	7.034517E-4	45.0	8
ATCGACT	20	7.034517E-4	45.0	21
TATACGG	130	0.0	41.538464	2
TCGACAG	100	0.0	40.5	1
ACGCACT	45	1.929584E-8	40.0	12
CGTTTTT	3445	0.0	39.579098	1
AACGGGC	300	0.0	39.0	4
GAACGTA	35	6.250564E-6	38.57143	37
ATAGCGG	345	0.0	38.47826	2
AATAGCG	65	9.094947E-12	38.076927	1
TACGGGA	630	0.0	37.857143	4