SRR3549830_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549830_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1750062
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	8871	0.5068963271015541	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	6138	0.3507304312647209	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5934	0.33907370138886506	No Hit
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	4632	0.264676337181197	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	3047	0.1741081173124152	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2295	0.13113821110337806	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	1879	0.10736762468986814	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	35	1.212411E-7	45.000004	20
CGTATAT	30	2.1661544E-6	45.000004	19
TTAGCGA	30	2.1661544E-6	45.000004	27
TAGGTCG	35	1.212411E-7	45.000004	1
GCGTAAT	30	2.1661544E-6	45.000004	10
TCGTTAC	20	7.034299E-4	45.0	43
CGTATCG	20	7.034299E-4	45.0	2
CCCCCGT	25	3.8916456E-5	45.0	3
ACTCGAT	20	7.034299E-4	45.0	44
TAGTACG	40	6.8193913E-9	45.0	1
GCGTTAG	90	0.0	42.5	1
TCTCGAT	55	6.184564E-11	40.909092	20
TCGACGG	110	0.0	40.909092	2
ACACGAC	205	0.0	40.609756	26
CGCGTAA	50	1.0822987E-9	40.5	31
CGTAAGG	190	0.0	40.263157	2
AAGTACG	135	0.0	40.000004	1
TAGTCCG	45	1.9292202E-8	40.0	14
CGTTTTT	3570	0.0	39.95798	1
CGTTAGG	260	0.0	39.80769	2