##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549830_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1750062
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.189250437984484	33.0	31.0	34.0	30.0	34.0
2	32.3628322882275	34.0	31.0	34.0	30.0	34.0
3	32.36473450654891	34.0	31.0	34.0	30.0	34.0
4	35.92213932992088	37.0	35.0	37.0	35.0	37.0
5	35.811725527438455	37.0	35.0	37.0	35.0	37.0
6	35.73489053530675	37.0	35.0	37.0	35.0	37.0
7	36.05918476031135	37.0	35.0	37.0	35.0	37.0
8	35.85820216655181	37.0	35.0	37.0	35.0	37.0
9	37.51974101488976	39.0	38.0	39.0	35.0	39.0
10	37.28744353057206	39.0	37.0	39.0	34.0	39.0
11	37.17366870430876	39.0	37.0	39.0	34.0	39.0
12	37.03324053662099	39.0	37.0	39.0	33.0	39.0
13	36.93964385261779	39.0	37.0	39.0	33.0	39.0
14	38.07128490304915	40.0	37.0	41.0	33.0	41.0
15	38.14644681159867	40.0	37.0	41.0	33.0	41.0
16	38.241269737872145	40.0	37.0	41.0	34.0	41.0
17	38.23882239600654	40.0	37.0	41.0	34.0	41.0
18	38.207761782154	40.0	37.0	41.0	34.0	41.0
19	38.165400997221816	40.0	37.0	41.0	34.0	41.0
20	38.12980568688424	40.0	36.0	41.0	34.0	41.0
21	38.04200708317762	40.0	36.0	41.0	34.0	41.0
22	37.967784569918095	40.0	36.0	41.0	34.0	41.0
23	37.79808372503374	40.0	36.0	41.0	33.0	41.0
24	37.55607858464443	39.0	35.0	41.0	33.0	41.0
25	37.46129679977052	39.0	35.0	41.0	33.0	41.0
26	37.6170924230113	39.0	35.0	41.0	33.0	41.0
27	37.710238265844296	39.0	36.0	41.0	33.0	41.0
28	37.66726778822693	40.0	36.0	41.0	33.0	41.0
29	37.673862983140026	40.0	36.0	41.0	33.0	41.0
30	37.54795258682264	40.0	36.0	41.0	33.0	41.0
31	37.45670496245276	40.0	35.0	41.0	33.0	41.0
32	37.380016250852826	39.0	35.0	41.0	33.0	41.0
33	37.25140937863916	39.0	35.0	41.0	33.0	41.0
34	37.180486748469484	39.0	35.0	41.0	32.0	41.0
35	37.09303670384249	39.0	35.0	41.0	32.0	41.0
36	37.01659141218997	39.0	35.0	41.0	32.0	41.0
37	36.96137165426139	39.0	35.0	41.0	32.0	41.0
38	36.85316291651382	39.0	35.0	41.0	31.0	41.0
39	36.84331069413541	39.0	35.0	41.0	31.0	41.0
40	36.72306181152439	39.0	35.0	41.0	31.0	41.0
41	36.658079542324785	39.0	35.0	41.0	31.0	41.0
42	36.585144412026544	39.0	35.0	41.0	31.0	41.0
43	36.52451398864726	39.0	35.0	41.0	31.0	41.0
44	36.416902372601655	39.0	35.0	41.0	31.0	41.0
45	36.32774895975114	39.0	35.0	40.0	31.0	41.0
46	36.27008071714031	38.0	35.0	40.0	31.0	41.0
47	36.146156536168434	38.0	35.0	40.0	30.0	41.0
48	36.09983760575339	38.0	35.0	40.0	30.0	41.0
49	36.06754160709735	38.0	35.0	40.0	30.0	41.0
50	35.94132836436652	38.0	35.0	40.0	30.0	41.0
51	34.9505948932095	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	18.0
10	30.0
11	30.0
12	35.0
13	34.0
14	41.0
15	73.0
16	98.0
17	216.0
18	458.0
19	690.0
20	1327.0
21	2033.0
22	3141.0
23	4607.0
24	6608.0
25	9467.0
26	13262.0
27	16137.0
28	18384.0
29	21121.0
30	25832.0
31	32398.0
32	42021.0
33	60317.0
34	113850.0
35	152966.0
36	136962.0
37	209873.0
38	371103.0
39	506608.0
40	312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75349216199197	21.9622504802687	24.38347898531595	11.900778372423378
2	32.92574777350745	24.359422694738814	24.188457323226263	18.52637220852747
3	31.130039964298405	24.37525070540358	26.833335047558315	17.661374282739697
4	28.966745178170832	25.121852825785602	26.876076390436452	19.035325605607113
5	25.668062045801804	29.638207103519758	24.861004924396966	19.83272592628147
6	26.948245262167852	32.959803709811425	24.48176121760258	15.610189810418145
7	79.55649571272332	5.698255261813581	9.588860280378638	5.15638874508446
8	78.65189918985728	5.094048096581721	10.098270804120082	6.155781909440923
9	72.84987617581548	6.894441454074199	12.578640071037483	7.677042299072832
10	40.500622263668376	25.4849828177516	18.965213803853807	15.04918111472622
11	30.88901993186527	24.080118304380076	24.959687142512664	20.07117462124199
12	28.248484910820302	21.91030946332187	29.794087295192973	20.047118330664855
13	24.469190234403122	24.105317411611704	31.443057445964772	19.9824349080204
14	20.19437025659662	27.836499506874613	29.794258717691147	22.17487151883762
15	18.099530188073338	25.26567630175388	35.79170337965169	20.84309013052109
16	20.783663664487314	25.04014143498916	33.37881743618226	20.797377464341263
17	20.899773836584075	24.018463345870032	28.812579211479367	26.269183606066527
18	22.533944511680158	24.655297926587743	30.83965025239106	21.97110730934104
19	23.979950424613527	25.822399434991443	28.003465020096428	22.194185120298595
20	26.15261630730797	25.109738969247946	27.806729133024998	20.930915590419083
21	23.92680945017948	25.409899763551238	29.028228714182696	21.63506207208659
22	21.621633976396264	25.181279291819376	27.329031771445813	25.868054960338547
23	21.83299791664524	26.31912469386799	28.210543397891048	23.637333991595728
24	23.41077058984196	22.70633840401083	29.97956643821762	23.903324567929594
25	21.683517498237208	25.229391872973643	28.318768135071785	24.768322493717363
26	22.081446257332598	26.36815152834585	28.3275678233114	23.222834391010146
27	22.466804033228538	25.689032731411803	28.217343156985297	23.626820078374365
28	20.119115779898085	25.999707438936447	30.00213706714391	23.87903971402156
29	23.613734827680393	24.540444852810932	28.19797241469159	23.647847904817088
30	23.305288612632012	23.63510549911946	28.155973902638877	24.903631985609653
31	23.303802950981165	25.05265527735589	27.054927196865027	24.58861457479792
32	25.033055971731287	25.364987069029553	27.360459229444444	22.241497729794716
33	23.184035765590018	24.123716759749083	28.573101981529796	24.1191454931311
34	21.540265430596172	23.7304164081044	30.369095494902464	24.36022266639696
35	22.86010438487322	23.090324799921376	29.412957940918666	24.63661287428674
36	22.4236055636886	24.350508724833748	29.9317967020597	23.294089009417952
37	22.187385361204345	24.575643605769397	30.496405270213284	22.740565762812974
38	22.059275614235382	25.132709584003308	29.022000363415696	23.786014438345614
39	23.454426186043694	22.385378346595722	30.33401102360945	23.826184443751135
40	23.947608713291302	21.50358101598686	31.09455550717632	23.45425476354552
41	19.88363840823925	23.337001774794263	30.094590934492604	26.68476888247388
42	22.76725053169545	22.22961243658796	30.047221184163764	24.955915847552827
43	24.13285929298505	21.954765031181754	29.776373637048287	24.136002038784913
44	23.11009552804415	23.18620711723356	28.84132105033993	24.86237630438236
45	22.84513348669933	21.638947648711873	28.370023462025916	27.145895402562882
46	23.314831131697048	22.85341890744442	29.051827878098035	24.779922082760496
47	20.638297386035468	22.59228530189216	32.185374003892434	24.58404330817994
48	21.56175038370069	21.123251633370703	31.95355364552799	25.36144433740062
49	22.65519735872215	20.019519308458786	33.225565722814395	24.099717610004674
50	20.573956808387358	21.145022290638845	31.58402388029681	26.69699702067698
51	19.98409199216942	20.886345740893752	29.20839375976394	29.92116850717289
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	430.0
1	991.5
2	1553.0
3	6247.0
4	10941.0
5	7570.0
6	4199.0
7	4201.0
8	4203.0
9	4122.5
10	4042.0
11	4124.5
12	4207.0
13	4205.5
14	4204.0
15	4266.0
16	4328.0
17	3997.0
18	3666.0
19	3592.0
20	3518.0
21	4288.5
22	5059.0
23	6117.0
24	7175.0
25	7981.0
26	10193.0
27	11599.0
28	15129.0
29	18659.0
30	22110.5
31	25562.0
32	26783.5
33	28005.0
34	31245.5
35	34486.0
36	38089.5
37	41693.0
38	46296.0
39	50899.0
40	57261.5
41	63624.0
42	74978.5
43	86333.0
44	99944.5
45	113556.0
46	134736.0
47	155916.0
48	173808.5
49	191701.0
50	191334.0
51	190967.0
52	164642.5
53	138318.0
54	121645.5
55	104973.0
56	96951.5
57	88930.0
58	82613.5
59	76297.0
60	77178.5
61	78060.0
62	68241.0
63	58422.0
64	50819.5
65	43217.0
66	36132.0
67	29047.0
68	23874.0
69	18701.0
70	16880.5
71	15060.0
72	11776.0
73	8492.0
74	6902.5
75	3962.5
76	2612.0
77	2122.0
78	1632.0
79	1316.5
80	1001.0
81	709.0
82	417.0
83	296.0
84	175.0
85	113.5
86	52.0
87	32.5
88	13.0
89	12.5
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1750062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.514794161551425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.90377859335662	14.394210250660786
2	12.32740381378804	5.304431111999474
3	5.733174529038651	3.7004420965354834
4	3.2486237596266285	2.79573885986769
5	2.1044297656546807	2.263818661775118
6	1.3766266840737253	1.7770703847087173
7	0.9707123193299888	1.4619273018326824
8	0.7524873280269356	1.295168797734028
9	0.5610633416400189	1.0864046076279494
>10	3.751583178330449	17.253664148717824
>50	1.5019312780359186	23.792794006000754
>100	0.7579465296677834	21.5065414729831
>500	0.00646666069302181	0.9133230856271796
>1k	0.0029638861509683296	1.2403918796042375
>5k	8.083325866277263E-4	1.2140733343249774
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	8871	0.5068963271015541	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	6138	0.3507304312647209	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5934	0.33907370138886506	No Hit
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	4632	0.264676337181197	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	3047	0.1741081173124152	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2295	0.13113821110337806	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	1879	0.10736762468986814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1428166544956694E-4	0.0	0.0	0.28016150284961333	0.0
2	1.1428166544956694E-4	0.0	0.0	1.5130321097195414	0.0
3	1.1428166544956694E-4	0.0	0.0	2.1730087276907906	0.0
4	1.1428166544956694E-4	0.0	0.0	2.892183248364915	0.0
5	1.1428166544956694E-4	0.0	0.0	4.790230288984048	0.0
6	1.1428166544956694E-4	0.0	0.0	5.787623524195142	0.0
7	1.1428166544956694E-4	0.0	0.0	6.823929666491816	0.0
8	1.1428166544956694E-4	0.0	0.0	8.484899392135821	0.0
9	1.1428166544956694E-4	0.0	0.0	9.102363230559831	0.0
10	1.1428166544956694E-4	0.0	0.0	10.442315757956004	0.0
11	1.714224981743504E-4	0.0	0.0	12.365447624141316	0.0
12	1.714224981743504E-4	0.0	0.0	13.815967662859945	0.0
13	1.714224981743504E-4	0.0	0.0	14.430974445476789	0.0
14	1.714224981743504E-4	0.0	0.0	14.652852298947122	0.0
15	1.714224981743504E-4	0.0	0.0	15.116321593177842	0.0
16	2.2856333089913387E-4	0.0	0.0	16.114057673385286	0.0
17	2.2856333089913387E-4	0.0	0.0	17.249331737961285	1.1428166544956694E-4
18	2.2856333089913387E-4	0.0	0.0	18.529400672661883	1.1428166544956694E-4
19	2.857041636239173E-4	0.0	0.0	19.25897482489192	1.1428166544956694E-4
20	2.857041636239173E-4	0.0	0.0	19.978777894726015	1.1428166544956694E-4
21	2.857041636239173E-4	0.0	0.0	20.870403448563536	1.1428166544956694E-4
22	2.857041636239173E-4	0.0	0.0	21.798084867850395	1.1428166544956694E-4
23	2.857041636239173E-4	0.0	0.0	22.703195658210966	1.1428166544956694E-4
24	3.428449963487008E-4	0.0	0.0	23.408656379031143	1.1428166544956694E-4
25	3.9998582907348424E-4	0.0	0.0	24.012063572604855	1.1428166544956694E-4
26	4.5712666179826774E-4	0.0	0.0	24.58130054820915	1.1428166544956694E-4
27	4.5712666179826774E-4	0.0	0.0	25.126538374069032	1.1428166544956694E-4
28	4.5712666179826774E-4	0.0	0.0	25.698403827978666	1.1428166544956694E-4
29	4.5712666179826774E-4	0.0	0.0	26.289182897520202	1.1428166544956694E-4
30	4.5712666179826774E-4	0.0	0.0	26.95401648627306	1.1428166544956694E-4
31	4.5712666179826774E-4	0.0	0.0	27.573365972176987	1.1428166544956694E-4
32	4.5712666179826774E-4	0.0	0.0	28.19540107721898	1.1428166544956694E-4
33	5.142674945230512E-4	0.0	0.0	28.7637809403324	1.1428166544956694E-4
34	5.142674945230512E-4	0.0	0.0	29.33478928175116	1.1428166544956694E-4
35	5.142674945230512E-4	0.0	0.0	29.92991105457978	1.1428166544956694E-4
36	5.142674945230512E-4	0.0	0.0	30.51451891418704	1.1428166544956694E-4
37	5.142674945230512E-4	0.0	0.0	31.088327156409317	1.1428166544956694E-4
38	5.142674945230512E-4	0.0	0.0	31.64985011959576	1.1428166544956694E-4
39	5.142674945230512E-4	0.0	0.0	32.20965885780047	1.1428166544956694E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	35	1.212411E-7	45.000004	20
CGTATAT	30	2.1661544E-6	45.000004	19
TTAGCGA	30	2.1661544E-6	45.000004	27
TAGGTCG	35	1.212411E-7	45.000004	1
GCGTAAT	30	2.1661544E-6	45.000004	10
TCGTTAC	20	7.034299E-4	45.0	43
CGTATCG	20	7.034299E-4	45.0	2
CCCCCGT	25	3.8916456E-5	45.0	3
ACTCGAT	20	7.034299E-4	45.0	44
TAGTACG	40	6.8193913E-9	45.0	1
GCGTTAG	90	0.0	42.5	1
TCTCGAT	55	6.184564E-11	40.909092	20
TCGACGG	110	0.0	40.909092	2
ACACGAC	205	0.0	40.609756	26
CGCGTAA	50	1.0822987E-9	40.5	31
CGTAAGG	190	0.0	40.263157	2
AAGTACG	135	0.0	40.000004	1
TAGTCCG	45	1.9292202E-8	40.0	14
CGTTTTT	3570	0.0	39.95798	1
CGTTAGG	260	0.0	39.80769	2
>>END_MODULE