FastQCFastQC Report
Sat 18 Jun 2016
SRR3549828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926164
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC91080.3112607495683769No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC82700.2826225734442772No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT59320.20272274554672945No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.19869016227388483No Hit
CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG35050.11978139297729042No Hit
TCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC32230.11014420244388216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT302.1666565E-645.00000442
AACGTCG207.0350774E-445.011
CGTTTTT43200.040.6770861
TAACGCG451.9301297E-840.01
CGTTCGG403.4606637E-739.3752
CTATCGG356.251599E-638.571432
TAGATCG356.251599E-638.571431
GCGTACG1700.038.382351
TTACGAG2000.038.251
TACGTTG950.037.8947371
TGCGTCG950.037.8947371
CGAGATA5300.037.3584919
CTACGCG850.037.0588231
TTTAGCG1950.036.923081
ATCTCGC552.752131E-936.8181830
CGAATAT3150.036.42857414
CGTTAGG3100.036.290322
TACGAAT3350.036.26865812
TACGAGG6900.036.1956522
CTACGTC250.0021076436.016
Produced by FastQC (version 0.11.5)