SRR3549825_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	958726
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5947	0.6203023595897055	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5157	0.5379013399031631	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3449	0.3597482492390944	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	3426	0.35734923221024567	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2296	0.2394844825320269	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1584	0.16521925972592796	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1548	0.16146427655033868	No Hit
GCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1113	0.11609156317863498	No Hit
AGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1002	0.10451369838723472	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	982	0.10242759662301847	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGA	30	2.165123E-6	45.000004	26
CTATGCA	20	7.0326997E-4	45.000004	11
TCGTTGA	20	7.0326997E-4	45.000004	25
ACACGAT	20	7.0326997E-4	45.000004	12
CCCTCCG	20	7.0326997E-4	45.000004	20
TCCGATA	20	7.0326997E-4	45.000004	11
CCGGCAT	40	6.8139343E-9	45.000004	15
CGTGACC	20	7.0326997E-4	45.000004	33
TAGAACG	30	2.165123E-6	45.000004	1
TCGGGTC	20	7.0326997E-4	45.000004	5
CCGTGAT	20	7.0326997E-4	45.000004	26
CCGTGAC	20	7.0326997E-4	45.000004	32
ATTAACG	20	7.0326997E-4	45.000004	1
TTATCGG	20	7.0326997E-4	45.000004	26
GCGTTAC	20	7.0326997E-4	45.000004	36
CCCACTA	20	7.0326997E-4	45.000004	14
CGTTATA	30	2.165123E-6	45.000004	14
CGTTAGC	30	2.165123E-6	45.000004	38
CGTTAGA	20	7.0326997E-4	45.000004	14
ACCTAGT	20	7.0326997E-4	45.000004	28