FastQCFastQC Report
Sat 18 Jun 2016
SRR3549823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723857
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC80590.4674981741524964No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74570.4325764840123049No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC68810.3991630396256767No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT50410.29242564783505826No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC27770.16109224837094957No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG26350.1528549061784127No Hit
ACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC20880.12112373590152781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC302.1661363E-645.00000445
CGCGATA302.1661363E-645.00000426
CGTTAGT302.1661363E-645.00000435
AATCTCG351.2123928E-745.00000418
ATCTCGC207.0342683E-445.014
GTCGAAC406.8193913E-945.034
CGAGTAA207.0342683E-445.020
ACGCATT207.0342683E-445.023
CTATCGG207.0342683E-445.044
CGTCTAT207.0342683E-445.016
GCGCATA253.8916198E-545.028
TACGACA253.8916198E-545.020
CCGAATA207.0342683E-445.033
TATCGCG750.045.01
GACTCGA253.8916198E-545.022
TCGCGGA207.0342683E-445.021
TACGTCA207.0342683E-445.023
TCTATCG207.0342683E-445.021
ATCGGGT650.044.9999964
CGTTTTT40350.041.5427471