##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1723857
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.209867175757616	33.0	31.0	34.0	30.0	34.0
2	32.394937631137616	34.0	31.0	34.0	30.0	34.0
3	32.38638239714779	34.0	31.0	34.0	30.0	34.0
4	35.935062479080344	37.0	35.0	37.0	35.0	37.0
5	35.82699783102659	37.0	35.0	37.0	35.0	37.0
6	35.754002797215776	37.0	35.0	37.0	35.0	37.0
7	36.06872089738302	37.0	35.0	37.0	35.0	37.0
8	35.90857478317517	37.0	35.0	37.0	35.0	37.0
9	37.60300187312521	39.0	38.0	39.0	35.0	39.0
10	37.34711637914282	39.0	37.0	39.0	34.0	39.0
11	37.25228252691493	39.0	37.0	39.0	34.0	39.0
12	37.137053711531756	39.0	37.0	39.0	34.0	39.0
13	37.0274233883669	39.0	37.0	39.0	33.0	39.0
14	38.1732608911296	40.0	37.0	41.0	33.0	41.0
15	38.251881101506676	40.0	37.0	41.0	33.0	41.0
16	38.35812773333287	40.0	37.0	41.0	34.0	41.0
17	38.35966266343438	40.0	37.0	41.0	34.0	41.0
18	38.32355526009408	40.0	37.0	41.0	34.0	41.0
19	38.281461281301176	40.0	37.0	41.0	34.0	41.0
20	38.21849376137348	40.0	37.0	41.0	34.0	41.0
21	38.134668362863046	40.0	36.0	41.0	34.0	41.0
22	38.061083372924784	40.0	36.0	41.0	34.0	41.0
23	37.88955696441178	40.0	36.0	41.0	33.0	41.0
24	37.63267602823204	39.0	35.0	41.0	33.0	41.0
25	37.57301156650465	39.0	35.0	41.0	33.0	41.0
26	37.719472090782475	39.0	36.0	41.0	33.0	41.0
27	37.77895614311396	40.0	36.0	41.0	33.0	41.0
28	37.723796115339034	40.0	36.0	41.0	33.0	41.0
29	37.73282702683575	40.0	36.0	41.0	33.0	41.0
30	37.59993665367835	40.0	36.0	41.0	33.0	41.0
31	37.52362289911518	40.0	35.0	41.0	33.0	41.0
32	37.419009813459006	40.0	35.0	41.0	33.0	41.0
33	37.26881057999591	39.0	35.0	41.0	33.0	41.0
34	37.19941793315803	39.0	35.0	41.0	32.0	41.0
35	37.08413748936252	39.0	35.0	41.0	32.0	41.0
36	36.987290129053626	39.0	35.0	41.0	32.0	41.0
37	36.90305112315001	39.0	35.0	41.0	31.0	41.0
38	36.81552878225978	39.0	35.0	41.0	31.0	41.0
39	36.85405692003455	39.0	35.0	41.0	31.0	41.0
40	36.73359797245363	39.0	35.0	41.0	31.0	41.0
41	36.66015568576744	39.0	35.0	41.0	31.0	41.0
42	36.52548268214823	39.0	35.0	41.0	31.0	41.0
43	36.43889081286905	39.0	35.0	41.0	31.0	41.0
44	36.379035500044374	39.0	35.0	41.0	31.0	41.0
45	36.35583113912581	39.0	35.0	41.0	31.0	41.0
46	36.29633490480939	39.0	35.0	40.0	31.0	41.0
47	36.16232494922723	38.0	35.0	40.0	30.0	41.0
48	36.09858532349261	38.0	35.0	40.0	30.0	41.0
49	36.06450303012373	38.0	35.0	40.0	30.0	41.0
50	35.94614924555807	38.0	35.0	40.0	30.0	41.0
51	34.96507482929268	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	35.0
11	28.0
12	23.0
13	31.0
14	37.0
15	58.0
16	100.0
17	205.0
18	384.0
19	742.0
20	1359.0
21	2025.0
22	3188.0
23	4725.0
24	6632.0
25	9577.0
26	13029.0
27	16132.0
28	18203.0
29	20822.0
30	24943.0
31	31568.0
32	40498.0
33	57430.0
34	106805.0
35	145886.0
36	133616.0
37	203473.0
38	364302.0
39	517627.0
40	350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.14434723993927	22.070566178053053	26.215109489940293	13.569977092067381
2	31.969821162660246	24.043699680425927	26.12009000746582	17.86638914944801
3	30.406292401283864	23.358434023239745	28.68306361838598	17.552209957090408
4	28.476782006860198	25.74540695660951	27.091284253856323	18.68652678267397
5	24.871378542419702	28.831393787303703	26.70407116135503	19.593156508921563
6	26.5135681207896	33.08041212235121	25.12540193299096	15.28061782386822
7	78.85224818531931	5.9499134789022525	9.60543710992269	5.592401225855741
8	79.78776661869284	4.62399143316412	9.300133363730287	6.288108584412743
9	74.39416378504714	6.228822924407303	11.934690638492636	7.442322652052925
10	39.98800364531397	24.439324143475936	19.094275221204544	16.47839699000555
11	30.0424571179628	24.776185031589044	25.118034732579325	20.063323117868826
12	28.412565543429647	20.597010076821917	30.270840330723487	20.71958404902495
13	23.636763374224195	24.10797415330854	31.687605178387766	20.567657294079496
14	19.28959304629096	27.790994264605473	30.31040277702849	22.609009912075074
15	17.755127020396703	24.729255384872413	36.73431148871397	20.781306106016913
16	19.586717459742893	23.28702438775374	34.707113177021064	22.419144975482304
17	20.074054866499946	23.376881028994863	29.874113688084336	26.67495041642085
18	21.36116858880986	24.408753162240256	30.76484882446746	23.465229424482427
19	22.88148030840145	25.87685637497774	28.451373866857864	22.790289449762945
20	25.504435692751777	25.06246167750573	27.74951750638249	21.68358512336
21	23.531882284899503	25.951572549231173	28.73620027647305	21.78034488939628
22	20.567251227915076	25.164500303679482	27.405985531282468	26.862262937122978
23	21.171361661669152	25.878770686895724	28.108247957922266	24.84161969351286
24	22.3864276445204	23.613443574496028	29.16709448637561	24.833034294607963
25	21.38007967018146	25.313758623830168	27.18670980249522	26.119451903493157
26	21.412332925526886	26.52267560476304	28.50758502590412	23.557406443805952
27	23.23899256144796	25.59040570070487	28.65678533660275	22.51381640124442
28	20.133572564313628	25.67585362358943	30.6174468067827	23.573127005314245
29	23.133125311438246	23.383378087625598	29.07340922129852	24.410087379637638
30	23.366613355980224	23.84026053205109	29.49548599448794	23.297640117480743
31	22.638594732625734	25.56064685179803	27.953246702017626	23.847511713558607
32	25.58825935097865	25.89072063402011	26.264533543095514	22.25648647190573
33	24.458931338272258	24.524946094716675	27.461732614712243	23.554389952298827
34	23.396256185982946	23.513319260240266	29.714065609850472	23.376358943926324
35	24.26987853400833	23.226346500898856	27.47339251457632	25.030382450516488
36	24.481729052931886	24.079723550155265	29.067318228832207	22.371229168080646
37	23.112763993765146	24.486543837452874	31.765047796888023	20.635644371893957
38	22.976905857040347	25.688383665234415	27.52003211403266	23.81467836369258
39	25.33939880164074	22.30869497875984	28.109408146963467	24.242498072635954
40	23.84919398766835	22.684538218657348	30.893281751328562	22.57298604234574
41	21.919741602696742	25.25209457629026	28.2236867675219	24.604477053491095
42	24.86702783351519	24.488922224987338	28.1643430980644	22.47970684343307
43	24.97788389640208	22.81795995839562	29.505347601338162	22.69880854386414
44	23.206739306102538	22.954688236901323	28.32218681711998	25.51638563987616
45	23.17315183335973	21.78597180624611	28.065727029562197	26.975149330831965
46	23.753652420125334	23.083121163762424	29.12335535952228	24.039871056589963
47	21.79960402748024	23.96074616397996	31.370525513427154	22.869124295112645
48	22.737326820032056	21.906747485435275	29.811695517667648	25.544230176865018
49	23.342423414471153	21.121531542349512	31.143070451899433	24.392974591279902
50	21.676914036373088	20.84755290026957	31.28913825218681	26.186394811170533
51	20.60814789161746	21.411520793198044	28.31766207985929	29.662669235325207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	550.0
1	1128.5
2	1707.0
3	7083.0
4	12459.0
5	8382.5
6	4306.0
7	4239.0
8	4172.0
9	4228.5
10	4285.0
11	4223.0
12	4161.0
13	4209.5
14	4258.0
15	4132.0
16	4006.0
17	3963.0
18	3920.0
19	4057.0
20	4194.0
21	4310.0
22	4426.0
23	4997.0
24	5568.0
25	6549.5
26	9132.0
27	10733.0
28	13369.5
29	16006.0
30	18937.5
31	21869.0
32	25081.5
33	28294.0
34	30998.0
35	33702.0
36	36438.5
37	39175.0
38	44194.5
39	49214.0
40	57752.5
41	66291.0
42	74286.5
43	82282.0
44	98097.5
45	113913.0
46	133573.5
47	153234.0
48	175568.5
49	197903.0
50	196165.0
51	194427.0
52	163894.0
53	133361.0
54	117123.0
55	100885.0
56	92562.0
57	84239.0
58	80677.5
59	77116.0
60	75124.5
61	73133.0
62	65751.0
63	58369.0
64	50850.0
65	43331.0
66	35349.0
67	27367.0
68	22707.0
69	18047.0
70	15405.5
71	12764.0
72	11276.5
73	9789.0
74	7307.0
75	4528.5
76	4232.0
77	3129.0
78	2026.0
79	1565.5
80	1105.0
81	742.5
82	380.0
83	294.0
84	208.0
85	133.0
86	58.0
87	39.0
88	20.0
89	15.5
90	11.0
91	8.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1723857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.729742739585646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.17155133162973	14.378907871156002
2	12.494685065016117	5.430125841498864
3	5.878156786307864	3.831925042482582
4	3.3471392138281666	2.909298961202601
5	2.164436681708641	2.3516326134825594
6	1.4172126891289702	1.8477400285229335
7	1.0205742527126007	1.5523771170662777
8	0.7758833584743872	1.3487796620459316
9	0.5472969718379348	1.0703360160173319
>10	3.9767644654777548	18.22470106089278
>50	1.5374354382895328	24.66709455371985
>100	0.6588402711872398	18.92988721909797
>500	0.0062308084114397495	0.879942381166865
>1k	0.0027090471354085867	0.9620617029308318
>5k	0.0010836188541634348	1.6151899287166471
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	8059	0.4674981741524964	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7457	0.4325764840123049	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	6881	0.3991630396256767	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	5041	0.29242564783505826	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	2777	0.16109224837094957	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	2635	0.1528549061784127	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	2088	0.12112373590152781	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.900472603005934E-4	0.0	0.0	0.3082622282474706	0.0
2	2.900472603005934E-4	0.0	0.0	1.5254165513728808	0.0
3	2.900472603005934E-4	0.0	0.0	2.213234624449708	0.0
4	3.4805671236071205E-4	0.0	0.0	2.939222916982093	0.0
5	3.4805671236071205E-4	0.0	0.0	4.824762146744191	0.0
6	3.4805671236071205E-4	0.0	0.0	5.923925244379319	0.0
7	3.4805671236071205E-4	0.0	0.0	7.120834268735748	0.0
8	3.4805671236071205E-4	0.0	0.0	9.143159786455605	0.0
9	3.4805671236071205E-4	0.0	0.0	9.90818844022445	0.0
10	3.4805671236071205E-4	0.0	0.0	11.451645931188027	0.0
11	3.4805671236071205E-4	0.0	0.0	13.642372888238409	0.0
12	3.4805671236071205E-4	0.0	0.0	15.288333080992217	0.0
13	3.4805671236071205E-4	0.0	0.0	15.931135819270391	0.0
14	3.4805671236071205E-4	0.0	0.0	16.172745187100787	0.0
15	3.4805671236071205E-4	0.0	0.0	16.622550478374947	0.0
16	4.0606616442083075E-4	0.0	0.0	17.599545669971466	0.0
17	4.0606616442083075E-4	0.0	0.0	18.77006039364054	0.0
18	4.0606616442083075E-4	0.0	0.0	20.15381786308261	0.0
19	4.0606616442083075E-4	0.0	0.0	20.869248435340054	0.0
20	4.0606616442083075E-4	0.0	0.0	21.59599085074922	0.0
21	4.0606616442083075E-4	0.0	0.0	22.510045786860513	0.0
22	4.0606616442083075E-4	0.0	0.0	23.48721500681321	0.0
23	4.0606616442083075E-4	0.0	0.0	24.411537615939142	0.0
24	5.22085068541068E-4	0.0	0.0	25.143384863129597	0.0
25	5.22085068541068E-4	0.0	0.0	25.76106950866574	0.0
26	5.22085068541068E-4	0.0	0.0	26.315407832552236	0.0
27	5.22085068541068E-4	0.0	0.0	26.867947863424867	0.0
28	5.22085068541068E-4	0.0	0.0	27.44235745772416	0.0
29	5.800945206011868E-4	0.0	0.0	28.0370703602445	0.0
30	5.800945206011868E-4	0.0	0.0	28.70348294551114	0.0
31	7.541228767815427E-4	0.0	0.0	29.33439374611699	0.0
32	7.541228767815427E-4	0.0	0.0	29.9335153669939	0.0
33	7.541228767815427E-4	0.0	0.0	30.489013879921593	0.0
34	7.541228767815427E-4	0.0	0.0	31.060581011069942	0.0
35	7.541228767815427E-4	0.0	0.0	31.672000635783593	0.0
36	7.541228767815427E-4	0.0	0.0	32.29304982953923	0.0
37	8.121323288416615E-4	0.0	0.0	32.87807515356552	0.0
38	8.701417809017801E-4	0.0	0.0	33.452194700604515	0.0
39	8.701417809017801E-4	0.0	0.0	34.006881081203375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGC	30	2.1661363E-6	45.000004	45
CGCGATA	30	2.1661363E-6	45.000004	26
CGTTAGT	30	2.1661363E-6	45.000004	35
AATCTCG	35	1.2123928E-7	45.000004	18
ATCTCGC	20	7.0342683E-4	45.0	14
GTCGAAC	40	6.8193913E-9	45.0	34
CGAGTAA	20	7.0342683E-4	45.0	20
ACGCATT	20	7.0342683E-4	45.0	23
CTATCGG	20	7.0342683E-4	45.0	44
CGTCTAT	20	7.0342683E-4	45.0	16
GCGCATA	25	3.8916198E-5	45.0	28
TACGACA	25	3.8916198E-5	45.0	20
CCGAATA	20	7.0342683E-4	45.0	33
TATCGCG	75	0.0	45.0	1
GACTCGA	25	3.8916198E-5	45.0	22
TCGCGGA	20	7.0342683E-4	45.0	21
TACGTCA	20	7.0342683E-4	45.0	23
TCTATCG	20	7.0342683E-4	45.0	21
ATCGGGT	65	0.0	44.999996	4
CGTTTTT	4035	0.0	41.542747	1
>>END_MODULE