FastQCFastQC Report
Sat 18 Jun 2016
SRR3549819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323331
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.30866202127231845No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.19144468052862237No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGG302.1606447E-645.0000042
GTTTCGC207.025757E-445.017
GTTCGCG253.8845657E-545.011
TCTCGTA253.8845657E-545.028
AACGAGC453.8380676E-1045.015
TCGTACG253.8845657E-545.030
CGTTTCG207.025757E-445.016
TCGCGTT253.8845657E-545.013
CGCTTAG207.025757E-445.02
GTACGAG207.025757E-445.01
CGTTTTA1950.042.692311
AGTACGG501.0768417E-940.52
TGCACGG501.0768417E-940.52
CGTTTTT3500.039.8571431
TACGAAT403.447949E-739.37512
CGAATAT403.447949E-739.37514
GCTACGA403.447949E-739.37510
AGCTACG403.447949E-739.3759
AGGCGAT1950.039.230777
ACCTATG356.234368E-638.57142634