##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549819_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323331
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01126090600654	33.0	31.0	34.0	30.0	34.0
2	32.19970556488552	33.0	31.0	34.0	30.0	34.0
3	32.092555307100156	33.0	31.0	34.0	30.0	34.0
4	35.84124627703499	37.0	35.0	37.0	35.0	37.0
5	32.13411952457389	37.0	35.0	37.0	0.0	37.0
6	33.97677302825897	37.0	35.0	37.0	19.0	37.0
7	20.654545960640952	32.0	0.0	37.0	0.0	37.0
8	28.025237295526875	35.0	17.0	37.0	17.0	37.0
9	34.8143697944212	37.0	32.0	39.0	32.0	39.0
10	36.22264799849071	37.0	35.0	39.0	32.0	39.0
11	36.59215788155172	38.0	35.0	39.0	32.0	39.0
12	36.94916045785897	39.0	37.0	39.0	33.0	39.0
13	36.74358165471297	39.0	35.0	39.0	33.0	39.0
14	38.06827368857301	40.0	37.0	41.0	33.0	41.0
15	38.25484719992825	40.0	37.0	41.0	33.0	41.0
16	38.33068279874185	40.0	38.0	41.0	34.0	41.0
17	38.2922639647915	40.0	37.0	41.0	34.0	41.0
18	38.18722918619	40.0	37.0	41.0	34.0	41.0
19	38.145946414046286	40.0	37.0	41.0	34.0	41.0
20	38.13547417352496	40.0	37.0	41.0	34.0	41.0
21	38.03167033164157	40.0	37.0	41.0	33.0	41.0
22	38.03335281801003	40.0	37.0	41.0	34.0	41.0
23	37.970837933881995	40.0	36.0	41.0	34.0	41.0
24	37.8266791616022	40.0	36.0	41.0	33.0	41.0
25	37.68698640093279	40.0	36.0	41.0	33.0	41.0
26	37.539815235780054	39.0	36.0	41.0	33.0	41.0
27	37.513597520806854	40.0	36.0	41.0	33.0	41.0
28	37.40204310752758	39.0	36.0	41.0	33.0	41.0
29	37.35789330438467	39.0	35.0	41.0	33.0	41.0
30	37.20455817722396	39.0	35.0	41.0	32.0	41.0
31	37.09791513959379	39.0	35.0	41.0	32.0	41.0
32	36.97553590592922	39.0	35.0	41.0	31.0	41.0
33	36.84835045201357	39.0	35.0	41.0	31.0	41.0
34	36.68221729435161	39.0	35.0	41.0	31.0	41.0
35	36.526723388725486	39.0	35.0	41.0	30.0	41.0
36	36.378655309883676	39.0	35.0	41.0	30.0	41.0
37	36.325712659782084	39.0	35.0	41.0	30.0	41.0
38	36.20834067874716	39.0	35.0	40.0	30.0	41.0
39	36.07491084987211	39.0	35.0	40.0	29.0	41.0
40	35.98656794430475	39.0	35.0	40.0	29.0	41.0
41	35.84483083898544	39.0	35.0	40.0	28.0	41.0
42	35.73718882507399	38.0	35.0	40.0	28.0	41.0
43	35.65031500227321	38.0	35.0	40.0	27.0	41.0
44	35.52250480158104	38.0	34.0	40.0	27.0	41.0
45	35.41786899493089	38.0	34.0	40.0	27.0	41.0
46	35.278943250105925	38.0	34.0	40.0	26.0	41.0
47	35.21971911137503	38.0	34.0	40.0	26.0	41.0
48	35.09275015386709	38.0	34.0	40.0	26.0	41.0
49	34.968914208659235	38.0	34.0	40.0	26.0	41.0
50	34.834275092706854	37.0	34.0	40.0	25.0	41.0
51	32.96882451729033	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	14.0
10	23.0
11	12.0
12	7.0
13	9.0
14	15.0
15	24.0
16	35.0
17	81.0
18	135.0
19	225.0
20	408.0
21	623.0
22	1003.0
23	1550.0
24	2219.0
25	2993.0
26	3656.0
27	4245.0
28	4564.0
29	5418.0
30	6826.0
31	8796.0
32	11804.0
33	16923.0
34	23665.0
35	29564.0
36	38153.0
37	61708.0
38	60632.0
39	37990.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.86050827170918	18.57879386758461	22.382635750979645	17.178062109726568
2	31.05640968543072	24.355227305764064	26.630913831336926	17.95744917746829
3	32.52456461026008	23.83254312144521	27.324011616578677	16.318880651716043
4	28.627319990969006	25.83544417330847	26.582666060476722	18.9545697752458
5	22.60810129557636	37.24542342058139	23.129548357565465	17.01692692627679
6	27.49875514565569	35.23293467066257	24.28471133296838	12.983598850713355
7	49.80901924034503	43.10226981019451	4.748694062740659	2.340016886719801
8	86.26825142037107	4.058070522158407	7.205619009621719	2.468059047848799
9	80.17480538519351	6.518397555446276	8.91532206933452	4.391474990025701
10	44.77949840875141	25.998435040252865	16.451871302164037	12.770195248831692
11	32.350130361765494	25.302244449186745	24.45821773971565	17.889407449332108
12	30.358054130287538	22.71696806059425	28.216286096909975	18.708691712208232
13	25.56204013843399	23.21954900705469	30.43692067880902	20.7814901757023
14	21.677476023022844	25.26513077929428	30.860016515583098	22.19737668209977
15	20.819531687342074	25.424100998666997	32.75374152184604	21.00262579214489
16	24.630487024133163	23.688418370029474	31.443319694059646	20.237774911777713
17	25.219047972511145	23.85419276221581	28.57969078127368	22.34706848399937
18	25.209769555038026	24.637909758111658	28.591134162823856	21.561186524026464
19	25.873794965530678	25.523380065629343	27.07349434480455	21.52933062403543
20	27.646900544643106	24.97471631238576	27.78855105139934	19.589832091571797
21	27.130092691390555	25.37801819188386	28.004738178522935	19.487150938202646
22	26.526067713890715	23.428004119617356	28.159069189159098	21.886858977332828
23	25.3842038035326	24.956159477439527	28.344946819203848	21.314689899824018
24	24.077184062153027	23.877698086481036	30.99022364078916	21.054894210576776
25	23.556355561328793	25.234512001633004	28.616185890001265	22.592946547036938
26	23.24058008666042	26.265344182896165	27.93174796106776	22.562327769375656
27	22.858618567350486	27.059576718594876	28.554948334678702	21.526856379375932
28	22.000364951087274	26.75307966139962	29.174128060717962	22.07242732679514
29	23.38717908273565	25.67245330636407	28.995363884069263	21.94500372683102
30	24.028936291292823	25.362554162761995	28.509174808478	22.099334737467178
31	25.030077536642015	25.52028725980497	27.363908811713074	22.08572639183994
32	25.627607621910677	25.597607405414262	27.87360321156957	20.90118176110549
33	25.223068620082827	25.602246614150825	27.504940757304436	21.669744008461915
34	23.229755266275117	26.47070648963446	28.226801636712846	22.07273660737758
35	23.78120254476063	26.430500013917623	28.27907005514473	21.509227386177013
36	24.625847815396607	27.739684719374264	26.467613683810086	21.16685378141904
37	24.52811515134645	27.427620611695136	27.079679956453294	20.964584280505115
38	24.209556151436146	26.91823549242108	27.071638661309926	21.80056969483285
39	23.46975699824638	26.74256412159675	27.156072260315277	22.631606619841588
40	24.97162350656139	25.548122512224314	27.91535609019859	21.564897891015708
41	22.05479833359622	25.6910101413103	28.876909420995823	23.377282104097656
42	22.838206048909633	25.864516548057566	27.707828819383234	23.58944858364957
43	22.80449446542398	25.675546112188442	28.24690487457126	23.273054547816326
44	22.694699858658772	26.26689058580835	28.0576251581197	22.980784397413174
45	22.968722454698128	25.98822878103244	27.19813441952674	23.844914344742694
46	22.75841165864084	26.953184198236478	27.255042046695184	23.033362096427503
47	22.756555975146213	26.427407208093257	28.68020697056577	22.135829846194767
48	22.733050650880987	25.973074032493017	28.78010459869298	22.513770717933017
49	22.690060649922216	26.497304619724062	28.51195833371993	22.30067639663379
50	21.78294070163393	26.55854217504662	28.420411281318525	23.238105842000923
51	21.892735308399132	26.51276864884592	27.947521270772057	23.64697477198289
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	586.0
2	964.0
3	2064.5
4	3165.0
5	2199.5
6	1234.0
7	1109.0
8	984.0
9	943.5
10	903.0
11	911.0
12	919.0
13	901.5
14	884.0
15	932.5
16	981.0
17	981.0
18	981.0
19	1075.5
20	1170.0
21	1278.0
22	1386.0
23	1549.5
24	1713.0
25	2019.0
26	2821.5
27	3318.0
28	4136.5
29	4955.0
30	5901.0
31	6847.0
32	7615.0
33	8383.0
34	9162.5
35	9942.0
36	10596.0
37	11250.0
38	12221.0
39	13192.0
40	14236.0
41	15280.0
42	16289.0
43	17298.0
44	17877.0
45	18456.0
46	18904.0
47	19352.0
48	20143.5
49	20935.0
50	20986.5
51	21038.0
52	20765.5
53	20493.0
54	20131.5
55	19770.0
56	19297.0
57	18824.0
58	18277.0
59	17730.0
60	16782.0
61	15834.0
62	14452.0
63	13070.0
64	11356.5
65	9643.0
66	8394.0
67	7145.0
68	5967.5
69	4790.0
70	3953.5
71	3117.0
72	2542.0
73	1967.0
74	1637.0
75	1034.5
76	762.0
77	589.0
78	416.0
79	298.5
80	181.0
81	146.0
82	111.0
83	78.5
84	46.0
85	36.0
86	26.0
87	22.0
88	18.0
89	14.0
90	10.0
91	7.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323331.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.218089012995556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.52176058264719	27.885071921360343
2	10.77332771997032	9.312052727356996
3	6.654103597051796	8.627329246772353
4	4.612277240361785	7.973352333062765
5	3.4871668166729437	7.535434294306782
6	2.5550109950066906	6.625361556682891
7	1.9929101807627774	6.029083871097756
8	1.47362246971124	5.094971765402457
9	1.111215902895266	4.322216502358545
>10	2.8004392791305097	15.499801819876943
>50	0.014532172631613426	0.4424631485282547
>100	0.0021798258947420142	0.1450801807097613
>500	0.0014532172631613427	0.5077806324841646
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	998	0.30866202127231845	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.19144468052862237	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0928058243719287E-4	0.0	0.0	0.011134100967738942	0.0
2	3.0928058243719287E-4	0.0	0.0	0.050412734937262435	0.0
3	3.0928058243719287E-4	0.0	0.0	0.06680460580643366	0.0
4	3.0928058243719287E-4	0.0	0.0	0.09989762812721328	0.0
5	3.0928058243719287E-4	0.0	0.0	0.21897065236553254	0.0
6	3.0928058243719287E-4	0.0	0.0	0.3204146834049318	0.0
7	3.0928058243719287E-4	0.0	0.0	0.40546684357515983	0.0
8	3.0928058243719287E-4	0.0	0.0	0.6086641862363955	0.0
9	3.0928058243719287E-4	0.0	0.0	0.7144381454299155	0.0
10	3.0928058243719287E-4	0.0	0.0	0.9451614599280613	0.0
11	3.0928058243719287E-4	0.0	0.0	1.2065035520874894	0.0
12	3.0928058243719287E-4	0.0	0.0	1.4375361471680723	0.0
13	3.0928058243719287E-4	0.0	0.0	1.5337224083060392	0.0
14	3.0928058243719287E-4	0.0	0.0	1.587846510232548	0.0
15	3.0928058243719287E-4	0.0	0.0	1.6648573752594091	0.0
16	3.0928058243719287E-4	0.0	0.0	1.8046521985210202	0.0
17	3.0928058243719287E-4	0.0	0.0	1.9546532810030588	0.0
18	3.0928058243719287E-4	0.0	0.0	2.1241390401786404	0.0
19	3.0928058243719287E-4	0.0	0.0	2.23548004985603	0.0
20	3.0928058243719287E-4	0.0	0.0	2.351769548852414	0.0
21	3.0928058243719287E-4	0.0	0.0	2.5088840847305085	0.0
22	3.0928058243719287E-4	0.0	0.0	2.667235742938351	0.0
23	3.0928058243719287E-4	0.0	0.0	2.833937976871998	0.0
24	3.0928058243719287E-4	0.0	0.0	2.9777534477052927	0.0
25	3.0928058243719287E-4	0.0	0.0	3.110125536988411	0.0
26	3.0928058243719287E-4	0.0	0.0	3.231982086468665	0.0
27	3.0928058243719287E-4	0.0	0.0	3.3748697155546483	0.0
28	3.0928058243719287E-4	0.0	0.0	3.5196130281352547	0.0
29	3.0928058243719287E-4	0.0	0.0	3.6507479950886244	0.0
30	3.0928058243719287E-4	0.0	0.0	3.8496154095957396	0.0
31	3.0928058243719287E-4	0.0	0.0	4.021575413430819	0.0
32	3.0928058243719287E-4	0.0	0.0	4.192916856101023	0.0
33	3.0928058243719287E-4	0.0	0.0	4.357144845375172	0.0
34	3.0928058243719287E-4	0.0	0.0	4.521991395814196	0.0
35	3.0928058243719287E-4	0.0	0.0	4.714982479255005	0.0
36	3.0928058243719287E-4	0.0	0.0	4.90611787920119	0.0
37	3.0928058243719287E-4	0.0	0.0	5.0817892500255155	0.0
38	3.0928058243719287E-4	0.0	0.0	5.2837494703570025	0.0
39	3.0928058243719287E-4	0.0	0.0	5.562411275132913	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGACGG	30	2.1606447E-6	45.000004	2
GTTTCGC	20	7.025757E-4	45.0	17
GTTCGCG	25	3.8845657E-5	45.0	11
TCTCGTA	25	3.8845657E-5	45.0	28
AACGAGC	45	3.8380676E-10	45.0	15
TCGTACG	25	3.8845657E-5	45.0	30
CGTTTCG	20	7.025757E-4	45.0	16
TCGCGTT	25	3.8845657E-5	45.0	13
CGCTTAG	20	7.025757E-4	45.0	2
GTACGAG	20	7.025757E-4	45.0	1
CGTTTTA	195	0.0	42.69231	1
AGTACGG	50	1.0768417E-9	40.5	2
TGCACGG	50	1.0768417E-9	40.5	2
CGTTTTT	350	0.0	39.857143	1
TACGAAT	40	3.447949E-7	39.375	12
CGAATAT	40	3.447949E-7	39.375	14
GCTACGA	40	3.447949E-7	39.375	10
AGCTACG	40	3.447949E-7	39.375	9
AGGCGAT	195	0.0	39.23077	7
ACCTATG	35	6.234368E-6	38.571426	34
>>END_MODULE