##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	312763
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03332875052356	33.0	31.0	34.0	30.0	34.0
2	32.20696501824065	33.0	31.0	34.0	30.0	34.0
3	32.08254493018676	33.0	31.0	34.0	30.0	34.0
4	35.839105009224234	37.0	35.0	37.0	35.0	37.0
5	32.112452559925565	37.0	35.0	37.0	0.0	37.0
6	33.98656490697429	37.0	35.0	37.0	19.0	37.0
7	20.792047013233663	32.0	0.0	37.0	0.0	37.0
8	28.108497488513667	35.0	17.0	37.0	17.0	37.0
9	34.888429897398346	37.0	32.0	39.0	32.0	39.0
10	36.27584145183414	37.0	35.0	39.0	32.0	39.0
11	36.6679658399491	38.0	35.0	39.0	33.0	39.0
12	37.03088600633707	39.0	37.0	39.0	34.0	39.0
13	36.818137055853796	39.0	37.0	39.0	33.0	39.0
14	38.24308182233832	40.0	38.0	41.0	34.0	41.0
15	38.40403116736954	40.0	38.0	41.0	34.0	41.0
16	38.47971147482279	40.0	38.0	41.0	34.0	41.0
17	38.44602462567503	40.0	38.0	41.0	34.0	41.0
18	38.34646041891144	40.0	37.0	41.0	34.0	41.0
19	38.287380540537086	40.0	37.0	41.0	34.0	41.0
20	38.305972893213074	40.0	37.0	41.0	34.0	41.0
21	38.212803304738735	40.0	37.0	41.0	34.0	41.0
22	38.21789661820612	40.0	37.0	41.0	34.0	41.0
23	38.158835923686624	40.0	37.0	41.0	34.0	41.0
24	38.04670948929381	40.0	37.0	41.0	34.0	41.0
25	37.924453979530824	40.0	36.0	41.0	33.0	41.0
26	37.7958901788255	40.0	36.0	41.0	33.0	41.0
27	37.764438248769835	40.0	36.0	41.0	33.0	41.0
28	37.70861642841385	40.0	36.0	41.0	33.0	41.0
29	37.693144649463015	40.0	36.0	41.0	33.0	41.0
30	37.573002561044625	40.0	36.0	41.0	33.0	41.0
31	37.48473125017985	40.0	36.0	41.0	33.0	41.0
32	37.39634163887672	40.0	36.0	41.0	33.0	41.0
33	37.25531472712565	40.0	36.0	41.0	32.0	41.0
34	37.11368352394625	40.0	35.0	41.0	32.0	41.0
35	36.99354463283701	40.0	35.0	41.0	31.0	41.0
36	36.87891150807481	40.0	35.0	41.0	31.0	41.0
37	36.847098282085796	39.0	35.0	41.0	31.0	41.0
38	36.74652372563251	39.0	35.0	41.0	31.0	41.0
39	36.67616374059592	39.0	35.0	41.0	31.0	41.0
40	36.57385624258624	39.0	35.0	41.0	30.0	41.0
41	36.492833231552325	39.0	35.0	41.0	30.0	41.0
42	36.42411986072521	39.0	35.0	41.0	30.0	41.0
43	36.34591367904771	39.0	35.0	41.0	30.0	41.0
44	36.24157269242206	39.0	35.0	40.0	30.0	41.0
45	36.1491544715967	39.0	35.0	40.0	30.0	41.0
46	36.0252491503151	39.0	35.0	40.0	29.0	41.0
47	35.97522085412916	39.0	35.0	40.0	29.0	41.0
48	35.887339614980036	39.0	35.0	40.0	29.0	41.0
49	35.77371364259839	38.0	35.0	40.0	28.0	41.0
50	35.65288413271391	38.0	35.0	40.0	28.0	41.0
51	33.92711094343001	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	9.0
10	19.0
11	10.0
12	5.0
13	4.0
14	8.0
15	12.0
16	23.0
17	56.0
18	89.0
19	186.0
20	291.0
21	516.0
22	718.0
23	1171.0
24	1642.0
25	2320.0
26	3027.0
27	3383.0
28	3932.0
29	4579.0
30	5699.0
31	7603.0
32	10079.0
33	14944.0
34	21180.0
35	26839.0
36	34305.0
37	60388.0
38	65702.0
39	44006.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.836387296451306	18.138974239280223	22.04097031937921	15.983668144889263
2	30.052787573977742	25.830421117587438	25.99124576756202	18.1255455408728
3	32.06229637137385	25.059549882818622	26.797926864750625	16.080226881056902
4	27.780779695808004	26.19907086196257	27.027173930420155	18.992975511809263
5	22.84413437650873	37.68220665487925	22.934618225301588	16.53904074331043
6	26.061906299658204	35.800590223268095	24.246154436426302	13.891349040647391
7	49.97074462132669	42.782554202383274	4.883569987498522	2.3631311887915127
8	86.08211329345224	4.540818447194841	6.9295281091433445	2.4475401502095835
9	80.37331781572628	7.303293548149877	8.25289436410317	4.070494272020667
10	39.47078139038185	33.23986532933883	15.338770890418624	11.950582389860694
11	29.447856683814898	26.224649335119565	27.80923574719516	16.518258233870377
12	28.57435182550366	24.2989100373126	29.11181949271493	18.014918644468818
13	23.329166173748174	27.229243868360385	30.03903914465586	19.40255081323558
14	19.829711314957333	28.272525842251163	30.349178131684372	21.548584711107132
15	20.03945479484466	27.864229464482694	33.27375680627184	18.822558934400806
16	23.05675543462622	26.572836300969104	31.606360087350488	18.76404817705419
17	22.39842948174816	26.78002193354073	29.831853512084226	20.989695072626876
18	23.16386529097112	25.872945329210932	30.2052992201763	20.757890159641647
19	23.395989934870812	28.945559417194488	27.45849093402992	20.19995971390478
20	25.628351179647208	27.00575195915118	29.42259794157237	17.943298919629243
21	24.38204007507282	28.32048547942052	28.736455399136084	18.561019046370575
22	23.962872846212626	25.688460591566137	29.119173303747566	21.22949325847367
23	22.27885011973923	28.64309397211307	29.521394794141248	19.556661114006452
24	21.15051972260146	26.300425561847153	32.36444208554081	20.184612630010584
25	21.211588327263776	28.121932581539376	29.67518536399766	20.99129372719919
26	21.249316575170337	29.055866582684015	28.445819997889778	21.248996844255874
27	20.564133225477438	29.660797472846852	29.266569255314728	20.508500046360982
28	19.727397422329368	28.9446002244511	30.75779424036731	20.570208112852225
29	20.636072681231475	27.182882885763345	30.73701173092725	21.44403270207793
30	20.655895997928145	28.863068841263196	30.1976256782292	20.28340948257946
31	23.068265747546864	27.3676873543226	28.737094860965012	20.826952037165523
32	23.263941067197848	28.336791756058098	28.32176440307837	20.077502773665685
33	22.36229988841391	28.903354936485453	28.39306439700348	20.341280778097154
34	20.80105383309407	28.33775094880149	29.45968672765001	21.401508490454432
35	21.472808484379545	28.149749171097604	29.53993918718007	20.83750315734278
36	21.939935350409094	30.740209040071875	26.926778423278968	20.39307718624006
37	21.197839897941893	30.24558531539856	28.83429305896158	19.72228172769797
38	21.61668739588762	29.3033383104779	28.989362552475832	20.09061174115864
39	21.393515217592874	29.37144099525839	28.412248251871226	20.82279553527751
40	22.992169789904814	28.111701192276577	28.511364835354563	20.38476418246404
41	20.005243586997185	27.524675233323638	30.314327462007974	22.15575371767121
42	20.639909452205025	27.707561316396124	28.938205606161855	22.714323625237
43	21.3116641034905	28.378037044023753	28.13152450897325	22.1787743435125
44	20.71824352624831	28.79944238928518	28.411289059127835	22.071025025338674
45	20.424091084942912	28.89344327813712	27.582546528841327	23.09991910807864
46	20.794019752975895	30.45053283156895	27.420123224294436	21.33532419116072
47	20.801693294922995	28.368125385675413	29.965181303415044	20.865000015986546
48	21.682871695181337	27.517641153205464	29.663994781991477	21.135492369621726
49	20.942374897286445	28.03816308195023	29.005668829113418	22.013793191649906
50	19.63563464987866	28.721428046156355	29.7666284055339	21.87630889843108
51	20.117788868887946	27.548655051908316	28.72462535530098	23.608930723902763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	191.0
1	484.0
2	777.0
3	2144.5
4	3512.0
5	2350.5
6	1189.0
7	1057.0
8	925.0
9	906.0
10	887.0
11	946.0
12	1005.0
13	1018.0
14	1031.0
15	1015.0
16	999.0
17	1062.5
18	1126.0
19	1159.5
20	1193.0
21	1337.0
22	1481.0
23	1674.0
24	1867.0
25	2441.0
26	3698.0
27	4381.0
28	5639.5
29	6898.0
30	7922.0
31	8946.0
32	9919.0
33	10892.0
34	11861.5
35	12831.0
36	13645.5
37	14460.0
38	15391.5
39	16323.0
40	17562.5
41	18802.0
42	19786.0
43	20770.0
44	21096.5
45	21423.0
46	22529.0
47	23635.0
48	22354.0
49	21073.0
50	20402.5
51	19732.0
52	18698.5
53	17665.0
54	16815.0
55	15965.0
56	15096.0
57	14227.0
58	13206.5
59	12186.0
60	11555.0
61	10924.0
62	9503.5
63	8083.0
64	6583.5
65	5084.0
66	4338.0
67	3592.0
68	2929.0
69	2266.0
70	1841.0
71	1416.0
72	1135.5
73	855.0
74	657.5
75	389.0
76	318.0
77	262.0
78	206.0
79	136.0
80	66.0
81	51.0
82	36.0
83	30.5
84	25.0
85	17.5
86	10.0
87	8.5
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	312763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.30381415028474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.84637221638985	25.48709761913174
2	10.250365921098814	8.0575695427056
3	5.854885250030908	6.903579652153747
4	4.018333161672968	6.317432791212815
5	3.0862100342438414	6.064991280733191
6	2.466536985238825	5.8166586757578225
7	1.9869573279957289	5.466650108086333
8	1.5881450195413889	4.9936125353404055
9	1.2852274670666306	4.546290735578524
>10	4.5866780211844915	24.496403764905978
>50	0.019646656564240847	0.5202620276630466
>100	0.009004717591943722	0.728752932997403
>500	8.186106901767019E-4	0.21138661235288908
>1k	8.186106901767019E-4	0.3893117213805111
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1210	0.38687440649949006	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	657	0.21006321080178922	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	336	0.10742958725936252	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01854439303881853	0.0
2	0.0	0.0	0.0	0.09336142702301743	0.0
3	0.0	0.0	0.0	0.11446366737753506	0.0
4	0.0	0.0	0.0	0.1691376537506035	0.0
5	0.0	0.0	0.0	0.3689694752895963	0.0
6	0.0	0.0	0.0	0.4712833679175606	0.0
7	0.0	0.0	0.0	0.5809510715781598	0.0
8	0.0	0.0	0.0	0.8133954463923162	0.0
9	0.0	0.0	0.0	0.8917295204356014	0.0
10	0.0	0.0	0.0	1.295549665401598	0.0
11	0.0	0.0	0.0	1.5458989714256481	0.0
12	0.0	0.0	0.0	1.8697863877760477	0.0
13	0.0	0.0	0.0	1.9570729274242797	0.0
14	0.0	0.0	0.0	1.9912841352717552	0.0
15	0.0	0.0	0.0	2.0779312130910625	0.0
16	0.0	0.0	0.0	2.2154155063098897	0.0
17	0.0	0.0	0.0	2.397342396638989	0.0
18	0.0	0.0	0.0	2.572235206849915	0.0
19	0.0	0.0	0.0	2.696610532575784	0.0
20	0.0	0.0	0.0	2.827380476590901	0.0
21	0.0	0.0	0.0	2.980211853703923	0.0
22	0.0	0.0	0.0	3.153186278428075	0.0
23	0.0	0.0	0.0	3.344385365276583	0.0
24	0.0	0.0	0.0	3.4754750402061627	0.0
25	0.0	0.0	0.0	3.613918526168377	0.0
26	0.0	0.0	0.0	3.7389333137231704	0.0
27	0.0	0.0	0.0	3.866825679508126	0.0
28	0.0	0.0	0.0	4.004309972726953	0.0
29	0.0	0.0	0.0	4.144991575090404	0.0
30	0.0	0.0	0.0	4.335870931024449	0.0
31	0.0	0.0	0.0	4.496056119170106	0.0
32	0.0	0.0	0.0	4.658479423717	0.0
33	0.0	0.0	0.0	4.819943535520506	0.0
34	0.0	0.0	0.0	4.988761458356647	0.0
35	0.0	0.0	0.0	5.183157854349779	0.0
36	0.0	0.0	0.0	5.355812548159469	0.0
37	0.0	0.0	0.0	5.53134482019932	0.0
38	0.0	0.0	0.0	5.71742821241643	0.0
39	0.0	0.0	0.0	5.935164965165317	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	20	7.025405E-4	45.0	5
TAGCGAA	20	7.025405E-4	45.0	1
CGACCCG	20	7.025405E-4	45.0	10
GGATACC	25	3.884273E-5	45.0	8
CGACAAG	25	3.884273E-5	45.0	2
TCGATGG	40	6.7884685E-9	45.0	2
TCTACGG	20	7.025405E-4	45.0	2
TCGTCAA	20	7.025405E-4	45.0	27
GCGATAC	20	7.025405E-4	45.0	9
CGTACAG	20	7.025405E-4	45.0	2
CGCACGG	20	7.025405E-4	45.0	2
ACCCGCT	25	3.884273E-5	45.0	12
TTCGCAG	20	7.025405E-4	45.0	2
AGGCGAT	140	0.0	43.392857	7
CCTCGTC	140	0.0	41.785713	36
TCGTCCC	140	0.0	41.785713	38
ACAACGA	55	6.002665E-11	40.90909	13
CACAACG	55	6.002665E-11	40.90909	12
CGTTTTA	200	0.0	40.5	1
CGTTATT	90	0.0	40.0	1
>>END_MODULE