FastQCFastQC Report
Sat 18 Jun 2016
SRR3549816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253790
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.37747744197958943No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.23562788131920093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAT600.045.00000412
CGTATGG207.022889E-445.02
TAAACGC253.8821883E-545.020
AAGCGAT207.022889E-445.06
AGCGATA207.022889E-445.07
TCTTTCG207.022889E-445.022
GTCCCGA207.022889E-445.038
TAGGCGA253.8821883E-545.06
TAGGATC406.7793735E-945.06
TTCCTAT207.022889E-445.043
AAGCTAG207.022889E-445.02
TGCGTAG253.8821883E-545.02
AGGCGAT1100.042.9545447
AACACGT950.042.6315841
ACACGTG900.042.542
CGTTGAT750.042.025
CACGACC750.042.027
ATCGTTG750.042.023
GTCAAGC750.042.016
CGACCAA700.041.78571729