##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549816_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	253790
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05113676661807	33.0	31.0	34.0	30.0	34.0
2	32.24095118010954	33.0	31.0	34.0	30.0	34.0
3	32.0971472477245	33.0	31.0	34.0	30.0	34.0
4	35.86261476023484	37.0	35.0	37.0	35.0	37.0
5	32.16168091729383	37.0	35.0	37.0	0.0	37.0
6	34.02197880137121	37.0	35.0	37.0	19.0	37.0
7	20.650281728988535	32.0	0.0	37.0	0.0	37.0
8	28.0378777729619	35.0	17.0	37.0	17.0	37.0
9	34.823688876630285	37.0	32.0	39.0	32.0	39.0
10	36.290373931202964	37.0	35.0	39.0	32.0	39.0
11	36.74688916032941	39.0	37.0	39.0	33.0	39.0
12	37.069498404192444	39.0	37.0	39.0	34.0	39.0
13	36.81503211316443	39.0	37.0	39.0	33.0	39.0
14	38.23650656054218	40.0	38.0	41.0	34.0	41.0
15	38.437795027384844	40.0	38.0	41.0	34.0	41.0
16	38.538267859253715	40.0	38.0	41.0	34.0	41.0
17	38.48949919224556	40.0	38.0	41.0	34.0	41.0
18	38.42329879033847	40.0	38.0	41.0	34.0	41.0
19	38.36634225146775	40.0	37.0	41.0	34.0	41.0
20	38.31490996493164	40.0	37.0	41.0	34.0	41.0
21	38.24823279089011	40.0	37.0	41.0	34.0	41.0
22	38.225186177548366	40.0	37.0	41.0	34.0	41.0
23	38.17529847511722	40.0	37.0	41.0	34.0	41.0
24	38.06133417392332	40.0	37.0	41.0	34.0	41.0
25	37.92060364868592	40.0	36.0	41.0	33.0	41.0
26	37.77979037787147	40.0	36.0	41.0	33.0	41.0
27	37.73825997872257	40.0	36.0	41.0	33.0	41.0
28	37.66687024705465	40.0	36.0	41.0	33.0	41.0
29	37.669195003743255	40.0	36.0	41.0	33.0	41.0
30	37.54875684621143	40.0	36.0	41.0	33.0	41.0
31	37.44229481067024	40.0	36.0	41.0	33.0	41.0
32	37.37662634461563	40.0	36.0	41.0	33.0	41.0
33	37.239493281847196	40.0	35.0	41.0	32.0	41.0
34	37.084688127979824	40.0	35.0	41.0	32.0	41.0
35	36.97258757240238	40.0	35.0	41.0	31.0	41.0
36	36.808889239134714	39.0	35.0	41.0	31.0	41.0
37	36.73744828401434	39.0	35.0	41.0	31.0	41.0
38	36.63458371094212	39.0	35.0	41.0	30.0	41.0
39	36.53959572875212	39.0	35.0	41.0	30.0	41.0
40	36.411442531226605	39.0	35.0	41.0	30.0	41.0
41	36.32964655817802	39.0	35.0	41.0	30.0	41.0
42	36.23593916229954	39.0	35.0	40.0	30.0	41.0
43	36.15857992828717	39.0	35.0	40.0	30.0	41.0
44	36.01555222822018	39.0	35.0	40.0	29.0	41.0
45	35.934843768469996	39.0	35.0	40.0	29.0	41.0
46	35.775408802553294	38.0	35.0	40.0	28.0	41.0
47	35.76747704795303	38.0	35.0	40.0	28.0	41.0
48	35.68077938453052	38.0	35.0	40.0	28.0	41.0
49	35.57653177824185	38.0	35.0	40.0	28.0	41.0
50	35.44577012490642	38.0	34.0	40.0	27.0	41.0
51	33.6429804168801	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	12.0
10	11.0
11	12.0
12	11.0
13	7.0
14	4.0
15	9.0
16	18.0
17	45.0
18	84.0
19	130.0
20	219.0
21	369.0
22	613.0
23	927.0
24	1464.0
25	2086.0
26	2577.0
27	2972.0
28	3255.0
29	3844.0
30	4650.0
31	6082.0
32	8043.0
33	12203.0
34	17541.0
35	22370.0
36	28040.0
37	49402.0
38	52369.0
39	34405.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.99448362819654	19.713148666220103	23.907955396193703	16.384412309389653
2	30.847945151503207	25.869813625438354	25.342606091650577	17.939635131407858
3	32.23452460695851	24.40797509752157	27.68075968320265	15.67674061231727
4	28.619330942905552	25.65467512510343	26.790653690058708	18.935340241932305
5	22.41735292958745	36.8458174080933	23.502896095196814	17.233933567122424
6	26.93053311793215	35.16017179557902	25.026202766066437	12.883092320422396
7	49.037787146853695	43.34331533945388	5.152291264431223	2.4666062492612
8	84.91981559557115	5.155443476890342	7.544426494345719	2.3803144331927975
9	77.80251388943616	8.38212695535679	9.403049765554199	4.4123093896528625
10	41.14937546790654	30.03900862918161	16.719728909728516	12.09188699318334
11	32.65337483746404	24.658575988021592	26.16218133102171	16.52586784349265
12	31.684857559399504	22.4863075771307	28.81713227471532	17.01170258875448
13	23.621498088971197	26.71618267071201	29.03069466882068	20.63162457149612
14	19.14653847669333	28.644154616021122	29.52125773277119	22.68804917451436
15	19.12053272390559	26.971511879900707	34.44304346112928	19.464911935064425
16	21.509515741360968	24.648725324086843	34.18377398636668	19.657984948185508
17	21.266401355451357	25.467906536900585	29.37704401276646	23.888648094881596
18	22.990267544032466	26.546751251034316	29.85618030655266	20.606800898380552
19	25.375310295913945	27.02982781039442	26.847787540880258	20.747074352811378
20	27.195318964498206	24.432010717522363	29.044485598329327	19.328184719650103
21	25.247251664762203	26.51838133890224	27.81157650025612	20.422790496079436
22	24.66369833326766	25.30477954214114	27.982978052720753	22.04854407187044
23	24.10221048898696	27.11966586547933	28.39158359273415	20.38654005279956
24	22.315300051223453	24.878442807045197	31.985499822688052	20.820757319043302
25	21.70652902005595	27.21974861105638	29.08428228062571	21.98944008826195
26	21.94255092793254	29.19185153079318	27.41794396942354	21.44765357185074
27	21.82985933251901	29.37664998620907	28.6370621379881	20.156428543283816
28	20.414909964931635	29.338035383584852	30.16312699475945	20.08392765672406
29	21.12297568856141	27.05189329760826	29.744276764253915	22.08085424957642
30	21.605264194806733	28.310808148469206	29.378620118996018	20.705307537728043
31	23.651838133890223	28.04168800977186	27.264667638598844	21.041806217739076
32	24.44934788604752	27.681153709760036	27.74853225107372	20.12096615311872
33	24.05847354111667	28.124827613381143	27.125576263840184	20.691122581662004
34	21.773907561369636	27.2701840104023	28.67055439536625	22.285354032861814
35	22.58402616336341	26.982938650065012	28.332873635683043	22.10016155088853
36	23.636865124709406	29.559872335395404	26.43484770873557	20.36841483115962
37	23.31297529453485	28.916820993734976	28.045628275345756	19.724575436384413
38	22.675046298120492	27.664604594349658	28.150439339611488	21.50990976791836
39	23.009968871901968	27.474289767130305	28.590567004216084	20.925174356751644
40	23.905197210291973	26.820599708420346	28.4916663383112	20.782536742976475
41	19.740730525237403	26.91910634776784	30.34871350329012	22.99144962370464
42	21.683281453169943	26.8225698412073	28.63430395208637	22.859844753536386
43	22.487489656802868	27.41715591630876	27.63347649631585	22.46187793057252
44	21.74711375546712	29.051972102919738	27.842704598289924	21.35820954332322
45	21.564679459395563	29.123684936364715	26.15233066708696	23.159304937152765
46	21.01264825249222	30.315615272469365	26.900193073013124	21.771543402025294
47	22.436660230899562	27.738287560581583	28.95267741045747	20.872374798061387
48	22.086764647937272	26.34146341463415	29.931045352456753	21.64072658497183
49	21.464990740375903	27.31076874581347	29.255289806532957	21.96895070727767
50	20.648961740021278	28.367941999290753	29.061428740297096	21.921667520390876
51	20.71712833444974	27.86634619173332	27.65830016943142	23.758225304385515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	419.0
2	718.0
3	1825.0
4	2932.0
5	2024.0
6	1116.0
7	972.5
8	829.0
9	859.0
10	889.0
11	880.5
12	872.0
13	795.0
14	718.0
15	765.0
16	812.0
17	781.5
18	751.0
19	856.5
20	962.0
21	1067.5
22	1173.0
23	1402.5
24	1632.0
25	1872.5
26	2593.0
27	3073.0
28	3870.0
29	4667.0
30	5581.0
31	6495.0
32	7430.0
33	8365.0
34	8779.5
35	9194.0
36	9523.5
37	9853.0
38	10789.0
39	11725.0
40	13083.0
41	14441.0
42	15428.0
43	16415.0
44	16798.0
45	17181.0
46	17430.0
47	17679.0
48	17351.5
49	17024.0
50	16311.5
51	15599.0
52	14785.0
53	13971.0
54	13456.0
55	12941.0
56	12935.0
57	12929.0
58	12538.0
59	12147.0
60	11389.5
61	10632.0
62	9462.0
63	8292.0
64	7009.5
65	5727.0
66	4703.5
67	3680.0
68	3010.0
69	2340.0
70	1933.5
71	1527.0
72	1246.5
73	966.0
74	777.0
75	461.0
76	334.0
77	260.5
78	187.0
79	142.5
80	98.0
81	71.5
82	45.0
83	32.0
84	19.0
85	14.5
86	10.0
87	5.5
88	1.0
89	1.5
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	253790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.45856810749044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.47375107577558	25.180267150005907
2	9.592844555551002	7.378541313684542
3	5.4075652637187	6.239016509712755
4	3.8553747797221427	5.9308877418338
5	3.017294373181427	5.80204105756728
6	2.5060448342281054	5.782733756255172
7	2.034752674070735	5.477757200835336
8	1.655669849596328	5.093975333937507
9	1.3923609688127536	4.819338823436699
>10	5.033605180115569	26.60940147365932
>50	0.019466415310847914	0.5240553213286575
>100	0.009220933568296381	0.5488789944442255
>500	0.002049096348510307	0.6131053232987903
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	958	0.37747744197958943	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	598	0.23562788131920093	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013002876393868946	0.0
2	0.0	0.0	0.0	0.06383230229717483	0.0
3	0.0	0.0	0.0	0.08944402852752276	0.0
4	0.0	0.0	0.0	0.13672721541431893	0.0
5	0.0	0.0	0.0	0.2608455809921589	0.0
6	0.0	0.0	0.0	0.3869340793569487	0.0
7	0.0	0.0	0.0	0.5031719137869892	0.0
8	0.0	0.0	0.0	0.753378777729619	0.0
9	0.0	0.0	0.0	0.8558256826510107	0.0
10	0.0	0.0	0.0	1.1509515741360967	0.0
11	0.0	0.0	0.0	1.4661728200480713	0.0
12	0.0	0.0	0.0	1.7423854367784388	0.0
13	0.0	0.0	0.0	1.8468024744867804	0.0
14	0.0	0.0	0.0	1.8858111036683873	0.0
15	0.0	0.0	0.0	1.9795894243271996	0.0
16	0.0	0.0	0.0	2.160841640726585	0.0
17	0.0	0.0	0.0	2.4019858938492455	0.0
18	0.0	0.0	0.0	2.653374837464045	0.0
19	0.0	0.0	0.0	2.7869498404192443	0.0
20	0.0	0.0	0.0	2.936679932227432	0.0
21	0.0	0.0	0.0	3.136057370266756	0.0
22	0.0	0.0	0.0	3.368533039126837	0.0
23	0.0	0.0	0.0	3.5753969817565703	0.0
24	0.0	0.0	0.0	3.7389180030734073	0.0
25	0.0	0.0	0.0	3.889436147996375	0.0
26	0.0	0.0	0.0	4.024587257181134	0.0
27	0.0	0.0	0.0	4.173529295874542	0.0
28	0.0	0.0	0.0	4.330351865715749	0.0
29	0.0	0.0	0.0	4.501753418180385	0.0
30	0.0	0.0	0.0	4.714921785728358	0.0
31	0.0	0.0	0.0	4.911147011308562	0.0
32	0.0	0.0	0.0	5.092793254265337	0.0
33	0.0	0.0	0.0	5.283502108042082	0.0
34	0.0	0.0	0.0	5.465148350998858	0.0
35	0.0	0.0	0.0	5.668860081169471	0.0
36	0.0	0.0	0.0	5.8623271208479455	0.0
37	0.0	0.0	0.0	6.077859647740258	0.0
38	0.0	0.0	0.0	6.2740848733204615	0.0
39	0.0	0.0	0.0	6.491981559557114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	60	0.0	45.000004	12
CGTATGG	20	7.022889E-4	45.0	2
TAAACGC	25	3.8821883E-5	45.0	20
AAGCGAT	20	7.022889E-4	45.0	6
AGCGATA	20	7.022889E-4	45.0	7
TCTTTCG	20	7.022889E-4	45.0	22
GTCCCGA	20	7.022889E-4	45.0	38
TAGGCGA	25	3.8821883E-5	45.0	6
TAGGATC	40	6.7793735E-9	45.0	6
TTCCTAT	20	7.022889E-4	45.0	43
AAGCTAG	20	7.022889E-4	45.0	2
TGCGTAG	25	3.8821883E-5	45.0	2
AGGCGAT	110	0.0	42.954544	7
AACACGT	95	0.0	42.63158	41
ACACGTG	90	0.0	42.5	42
CGTTGAT	75	0.0	42.0	25
CACGACC	75	0.0	42.0	27
ATCGTTG	75	0.0	42.0	23
GTCAAGC	75	0.0	42.0	16
CGACCAA	70	0.0	41.785717	29
>>END_MODULE