FastQCFastQC Report
Sat 18 Jun 2016
SRR3549815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215468
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.5703863218668201No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6390.29656375888763065No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.10349564668535467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG351.2057899E-745.0000045
CGACGCG253.880224E-545.015
CCGATCT207.020517E-445.024
TAGACGG207.020517E-445.02
GCAACGA253.880224E-545.011
ATCAGAG207.020517E-445.02
GGCAACG207.020517E-445.01
CGCGCAA253.880224E-545.018
TACGAAT502.1827873E-1145.012
CGGGCAA207.020517E-445.06
CGAATAT502.1827873E-1145.014
AACGACG253.880224E-545.013
GCGATGT502.1827873E-1145.09
TAGGCGA406.7702786E-945.06
GCTACGA551.8189894E-1245.010
CGCAATC253.880224E-545.020
GTAAGGT253.880224E-545.04
ATACGGC253.880224E-545.014
ACGACGC253.880224E-545.014
CGTTTTA2100.043.928571
Produced by FastQC (version 0.11.5)