##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	215468
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9229444743535	31.0	31.0	34.0	30.0	34.0
2	32.108354836913136	33.0	31.0	34.0	30.0	34.0
3	31.9276644327696	33.0	31.0	34.0	30.0	34.0
4	35.73499081070043	37.0	35.0	37.0	35.0	37.0
5	32.049274138155084	37.0	35.0	37.0	0.0	37.0
6	33.93137264002079	37.0	35.0	37.0	19.0	37.0
7	20.77719197282195	32.0	0.0	37.0	0.0	37.0
8	28.09750867878293	35.0	17.0	37.0	17.0	37.0
9	34.82173222937977	37.0	32.0	39.0	32.0	39.0
10	36.26131954628994	37.0	35.0	39.0	32.0	39.0
11	36.65304360740342	38.0	35.0	39.0	33.0	39.0
12	36.90583752575788	39.0	35.0	39.0	33.0	39.0
13	36.5330814784562	39.0	35.0	39.0	32.0	39.0
14	37.8965693281601	40.0	37.0	41.0	33.0	41.0
15	38.13095215994951	40.0	37.0	41.0	33.0	41.0
16	38.234461729816026	40.0	37.0	41.0	34.0	41.0
17	38.19880910390406	40.0	37.0	41.0	34.0	41.0
18	38.14658325134127	40.0	37.0	41.0	34.0	41.0
19	38.07672601035885	40.0	37.0	41.0	34.0	41.0
20	38.031057976126384	40.0	36.0	41.0	34.0	41.0
21	37.89656468709971	40.0	36.0	41.0	33.0	41.0
22	37.84189299571166	40.0	36.0	41.0	33.0	41.0
23	37.80898323648987	40.0	36.0	41.0	33.0	41.0
24	37.69407986336718	39.0	35.0	41.0	33.0	41.0
25	37.56297918948521	39.0	35.0	41.0	33.0	41.0
26	37.41280839846288	39.0	35.0	41.0	33.0	41.0
27	37.33145525089572	39.0	35.0	41.0	33.0	41.0
28	37.2642480553957	39.0	35.0	41.0	33.0	41.0
29	37.24643102456049	39.0	35.0	41.0	33.0	41.0
30	37.114402138600624	39.0	35.0	41.0	32.0	41.0
31	36.957218705329794	39.0	35.0	41.0	32.0	41.0
32	36.86895037778232	39.0	35.0	41.0	31.0	41.0
33	36.69708262943917	39.0	35.0	41.0	31.0	41.0
34	36.478804277201256	39.0	35.0	41.0	30.0	41.0
35	36.37538752854252	39.0	35.0	41.0	30.0	41.0
36	36.19775094213526	39.0	35.0	41.0	30.0	41.0
37	36.07133309818627	39.0	35.0	40.0	29.0	41.0
38	35.94460430319119	39.0	35.0	40.0	29.0	41.0
39	35.9004074851022	39.0	35.0	40.0	29.0	41.0
40	35.762846455158076	39.0	35.0	40.0	28.0	41.0
41	35.62761523752947	38.0	35.0	40.0	27.0	41.0
42	35.515798169565784	38.0	35.0	40.0	27.0	41.0
43	35.39364081905434	38.0	34.0	40.0	26.0	41.0
44	35.23383054560306	38.0	34.0	40.0	26.0	41.0
45	35.12918391594111	38.0	34.0	40.0	25.0	41.0
46	34.97021367442033	38.0	34.0	40.0	24.0	41.0
47	34.95912618114987	38.0	34.0	40.0	24.0	41.0
48	34.82558895056342	38.0	34.0	40.0	24.0	41.0
49	34.70941856795441	37.0	34.0	40.0	24.0	41.0
50	34.542377522416324	37.0	34.0	40.0	24.0	41.0
51	32.63816436779475	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	8.0
11	6.0
12	0.0
13	3.0
14	6.0
15	6.0
16	20.0
17	41.0
18	84.0
19	170.0
20	276.0
21	456.0
22	729.0
23	1062.0
24	1526.0
25	2209.0
26	2765.0
27	3156.0
28	3639.0
29	3868.0
30	4768.0
31	6034.0
32	8011.0
33	12386.0
34	16664.0
35	20656.0
36	23687.0
37	38750.0
38	39935.0
39	24532.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.936157573282344	17.494941244175468	22.63213098928843	17.936770193253754
2	32.59277479718566	23.360777470436446	26.148198340356803	17.89824939202109
3	32.137486772977894	22.98531568492769	28.03293296452373	16.844264577570684
4	27.904375591735196	26.165370263797872	26.98219689234596	18.948057252120964
5	22.084021757291104	37.53596821801845	23.383982772383835	16.996027252306607
6	27.191972821950362	34.56568956875267	25.224627322850722	13.017710286446246
7	48.62206917036404	43.114522806170754	5.7428481259398145	2.5205598975253865
8	82.95895446191545	5.125587094139269	8.92568734104368	2.989771102901591
9	76.85085488332373	7.426624835242356	10.978428351309708	4.744091930124195
10	44.45300462249615	26.21363729184844	17.570590528523958	11.762767557131454
11	36.535819704085995	24.134442237362393	23.95297677613381	15.376761282417807
12	34.519743070896844	21.50203278445059	26.52830118625504	17.449922958397536
13	25.75695694952383	26.263760744054803	27.67835595076763	20.30092635565374
14	19.42283772996454	29.472125791300797	28.745335734308576	22.359700744426085
15	19.098891714778993	26.304137969443257	35.92087920248018	18.676091113297566
16	22.6075333692242	23.50000928212078	35.439601240091335	18.452856108563683
17	20.637867339929826	24.852878385653554	29.51946460727347	24.98978966714315
18	22.973248927915048	24.345610485083633	30.565095513022815	22.1160450739785
19	26.265153062171642	26.86199342825849	26.64015074164145	20.232702767928416
20	29.13379248890787	23.655484805168285	27.818980080568807	19.39174262535504
21	25.685484619525866	26.511129262813967	28.457125884122004	19.34626023353816
22	23.903317429966396	25.118811145970632	27.59296043960124	23.38491098446173
23	23.262386990179515	27.87792154751518	28.24456531828392	20.615126144021385
24	22.884140568437076	23.370987803293296	32.01589099077357	21.728980637496058
25	21.701598381198135	24.902073625782016	30.307052555367854	23.089275437651995
26	22.173594222808028	27.18965229175562	28.775502626840183	21.861250858596172
27	21.832012178142463	28.346668646852436	28.7290920229454	21.092227152059703
28	20.242913100785266	28.0811999925743	31.21623628566655	20.45965062097388
29	21.67560844301706	26.01917686152932	30.91920842073997	21.386006274713644
30	21.950823324113095	26.562180927098222	30.195667106020384	21.2913286427683
31	23.749698331074683	26.269330016522176	28.051497206081642	21.929474446321496
32	26.0344923608146	26.472608461581302	27.74750775057085	19.745391427033248
33	24.301520411383592	26.381179571908593	28.006943026342658	21.310356990365158
34	21.2077895557577	26.46054170456866	29.771010080383164	22.560658659290475
35	21.50620973880112	25.69987190673325	29.523177455585053	23.270740898880575
36	23.94091001912117	28.528134108081016	26.90329886572484	20.627657007072976
37	22.955612898435035	28.960680936380346	28.551339410028405	19.532366755156218
38	21.918800007425695	28.436241112369352	27.786492657842466	21.858466222362484
39	21.72016262275605	27.855180351606734	27.9452169231626	22.479440102474612
40	24.543783763714334	25.367107876807694	28.554124046262093	21.534984313215883
41	19.656747173594223	25.253866003304438	30.035086416544456	25.05430040655689
42	21.53823305548852	26.189039671784208	27.765607886089818	24.50711938663746
43	22.88785341674866	26.176044702693673	27.13628009727663	23.799821783281043
44	21.152096831083966	27.487608368760096	27.53448307869382	23.82581172146212
45	21.081552713163905	28.038966343030054	26.743646388326802	24.135834555479235
46	21.529879146787458	29.002914585924593	26.44940315963391	23.017803107654036
47	21.476042846269515	26.339410028403286	29.378840477472295	22.8057066478549
48	22.00373141255314	25.14155234187907	30.611506116917592	22.243210128650194
49	20.809586574340504	25.98436890860824	29.7849332615516	23.421111255499657
50	19.579241465089943	26.91582972877643	29.496259305326085	24.008669500807546
51	19.199138619191714	27.54562162362857	27.176657322665083	26.078582434514637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	388.0
2	631.0
3	2056.0
4	3481.0
5	2309.0
6	1137.0
7	1010.0
8	883.0
9	851.0
10	819.0
11	796.0
12	773.0
13	784.0
14	795.0
15	737.0
16	679.0
17	725.0
18	771.0
19	827.5
20	884.0
21	874.0
22	864.0
23	945.5
24	1027.0
25	1170.0
26	1452.0
27	1591.0
28	2269.0
29	2947.0
30	3589.5
31	4232.0
32	4755.5
33	5279.0
34	5900.0
35	6521.0
36	6910.0
37	7299.0
38	7812.0
39	8325.0
40	9608.5
41	10892.0
42	12169.0
43	13446.0
44	14454.5
45	15463.0
46	15666.5
47	15870.0
48	15448.0
49	15026.0
50	14803.0
51	14580.0
52	13695.5
53	12811.0
54	12456.0
55	12101.0
56	11770.5
57	11440.0
58	11281.0
59	11122.0
60	10558.5
61	9995.0
62	8906.5
63	7818.0
64	6475.0
65	5132.0
66	4235.0
67	3338.0
68	2809.5
69	2281.0
70	1884.0
71	1487.0
72	1238.0
73	989.0
74	792.0
75	462.5
76	330.0
77	247.5
78	165.0
79	127.5
80	90.0
81	66.5
82	43.0
83	40.5
84	38.0
85	23.5
86	9.0
87	7.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	215468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.18709042642063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34925911318948	28.5629420610021
2	8.989802242379852	7.405275957450759
3	4.734914643078484	5.850520726975699
4	3.304974928164967	5.444892048935341
5	2.5240858639923376	5.197987636215123
6	2.0744830694687026	5.126515306217164
7	1.712772550566229	4.9380882544043665
8	1.5189588145811033	5.004919524012847
9	1.1425995830751028	4.235431711437429
>10	4.621105414389543	26.376074405480164
>50	0.014648712603526959	0.446934115506711
>100	0.010141416417826357	0.5434681716078489
>500	0.0011268240464251506	0.29656375888763065
>1k	0.0011268240464251506	0.5703863218668201
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1229	0.5703863218668201	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	639	0.29656375888763065	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	223	0.10349564668535467	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04548239181688232	0.0
2	0.0	0.0	0.0	0.16707817402120037	0.0
3	0.0	0.0	0.0	0.22369911077282936	0.0
4	0.0	0.0	0.0	0.28542521395288395	0.0
5	0.0	0.0	0.0	0.4775651140772644	0.0
6	0.0	0.0	0.0	0.6256149405016058	0.0
7	0.0	0.0	0.0	0.7908366903670151	0.0
8	0.0	0.0	0.0	1.2396272300295172	0.0
9	0.0	0.0	0.0	1.403920767817031	0.0
10	0.0	0.0	0.0	1.8290418994931963	0.0
11	0.0	0.0	0.0	2.3674049046726195	0.0
12	0.0	0.0	0.0	2.788349081998255	0.0
13	0.0	0.0	0.0	2.960532422447881	0.0
14	0.0	0.0	0.0	3.0324688584847865	0.0
15	0.0	0.0	0.0	3.186552063415449	0.0
16	0.0	0.0	0.0	3.55412404626209	0.0
17	0.0	0.0	0.0	3.918911392875044	0.0
18	0.0	0.0	0.0	4.346817160784896	0.0
19	0.0	0.0	0.0	4.583975346687211	0.0
20	0.0	0.0	0.0	4.821133532589526	0.0
21	0.0	0.0	0.0	5.156218092709822	0.0
22	0.0	0.0	0.0	5.481092319973268	0.0
23	0.0	0.0	0.0	5.832420591456736	0.0
24	0.0	0.0	0.0	6.10531494235803	0.0
25	0.0	0.0	0.0	6.335975643715076	0.0
26	0.0	0.0	0.0	6.568956875266861	0.0
27	0.0	0.0	0.0	6.8232869846102435	0.0
28	0.0	0.0	0.0	7.075296563758887	0.0
29	0.0	0.0	0.0	7.336588263686487	0.0
30	0.0	0.0	0.0	7.612731356860415	0.0
31	0.0	0.0	0.0	7.905118161397516	0.0
32	0.0	0.0	0.0	8.165945755286169	0.0
33	0.0	0.0	0.0	8.424916925019028	0.0
34	0.0	0.0	0.0	8.7024523363098	0.0
35	0.0	0.0	0.0	9.012011064287968	0.0
36	0.0	0.0	0.0	9.308110717136652	0.0
37	0.0	0.0	0.0	9.600033415634805	0.0
38	0.0	0.0	0.0	9.904486977184547	0.0
39	0.0	0.0	0.0	10.232609946720627	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	35	1.2057899E-7	45.000004	5
CGACGCG	25	3.880224E-5	45.0	15
CCGATCT	20	7.020517E-4	45.0	24
TAGACGG	20	7.020517E-4	45.0	2
GCAACGA	25	3.880224E-5	45.0	11
ATCAGAG	20	7.020517E-4	45.0	2
GGCAACG	20	7.020517E-4	45.0	1
CGCGCAA	25	3.880224E-5	45.0	18
TACGAAT	50	2.1827873E-11	45.0	12
CGGGCAA	20	7.020517E-4	45.0	6
CGAATAT	50	2.1827873E-11	45.0	14
AACGACG	25	3.880224E-5	45.0	13
GCGATGT	50	2.1827873E-11	45.0	9
TAGGCGA	40	6.7702786E-9	45.0	6
GCTACGA	55	1.8189894E-12	45.0	10
CGCAATC	25	3.880224E-5	45.0	20
GTAAGGT	25	3.880224E-5	45.0	4
ATACGGC	25	3.880224E-5	45.0	14
ACGACGC	25	3.880224E-5	45.0	14
CGTTTTA	210	0.0	43.92857	1
>>END_MODULE