##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	349068
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02921207329231	33.0	31.0	34.0	30.0	34.0
2	32.21262046363459	33.0	31.0	34.0	30.0	34.0
3	32.07223234441427	33.0	31.0	34.0	30.0	34.0
4	35.83913163051325	37.0	35.0	37.0	35.0	37.0
5	32.18824125958266	37.0	35.0	37.0	10.0	37.0
6	34.026215522476996	37.0	35.0	37.0	19.0	37.0
7	20.69262722449494	32.0	0.0	37.0	0.0	37.0
8	28.058117616051888	35.0	17.0	37.0	17.0	37.0
9	34.8754769844271	37.0	32.0	39.0	32.0	39.0
10	36.297234349754206	37.0	35.0	39.0	32.0	39.0
11	36.70523221836433	38.0	35.0	39.0	33.0	39.0
12	36.995502309005694	39.0	37.0	39.0	33.0	39.0
13	36.73792785359873	39.0	35.0	39.0	33.0	39.0
14	38.069169903858274	40.0	37.0	41.0	33.0	41.0
15	38.2614161137658	40.0	38.0	41.0	33.0	41.0
16	38.35340678607034	40.0	38.0	41.0	34.0	41.0
17	38.32781005420147	40.0	37.0	41.0	34.0	41.0
18	38.24961325586992	40.0	37.0	41.0	34.0	41.0
19	38.20615467473386	40.0	37.0	41.0	34.0	41.0
20	38.18064388600502	40.0	37.0	41.0	34.0	41.0
21	38.072470120434986	40.0	37.0	41.0	34.0	41.0
22	38.043570307218076	40.0	37.0	41.0	34.0	41.0
23	37.9853180469135	40.0	36.0	41.0	34.0	41.0
24	37.84600994648607	40.0	36.0	41.0	33.0	41.0
25	37.70316098868988	40.0	36.0	41.0	33.0	41.0
26	37.5544650326011	40.0	35.0	41.0	33.0	41.0
27	37.520743809229145	40.0	35.0	41.0	33.0	41.0
28	37.41599344540319	39.0	35.0	41.0	33.0	41.0
29	37.37718725291347	39.0	35.0	41.0	33.0	41.0
30	37.235043028865434	39.0	35.0	41.0	32.0	41.0
31	37.110239265701814	39.0	35.0	41.0	32.0	41.0
32	36.9915374654795	39.0	35.0	41.0	31.0	41.0
33	36.840188731135484	39.0	35.0	41.0	31.0	41.0
34	36.65631051829443	39.0	35.0	41.0	31.0	41.0
35	36.51817983888526	39.0	35.0	41.0	30.0	41.0
36	36.35198299471736	39.0	35.0	41.0	30.0	41.0
37	36.274402122222604	39.0	35.0	41.0	30.0	41.0
38	36.138855466556656	39.0	35.0	41.0	29.0	41.0
39	36.03650005156588	39.0	35.0	40.0	29.0	41.0
40	35.91010061076925	39.0	35.0	40.0	28.0	41.0
41	35.763848304628326	39.0	35.0	40.0	28.0	41.0
42	35.67786505781108	38.0	35.0	40.0	27.0	41.0
43	35.59394158158296	38.0	35.0	40.0	27.0	41.0
44	35.464614344483024	38.0	34.0	40.0	27.0	41.0
45	35.345256511625244	38.0	34.0	40.0	26.0	41.0
46	35.233475998945764	38.0	34.0	40.0	26.0	41.0
47	35.17556751120126	38.0	34.0	40.0	26.0	41.0
48	35.040994877788854	38.0	34.0	40.0	26.0	41.0
49	34.92214124468585	38.0	34.0	40.0	26.0	41.0
50	34.775628244353534	37.0	34.0	40.0	24.0	41.0
51	32.971913781841934	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	16.0
10	15.0
11	15.0
12	10.0
13	11.0
14	8.0
15	12.0
16	36.0
17	63.0
18	146.0
19	232.0
20	426.0
21	682.0
22	1091.0
23	1695.0
24	2466.0
25	3461.0
26	4087.0
27	4615.0
28	5136.0
29	5916.0
30	7138.0
31	9352.0
32	12310.0
33	18400.0
34	25711.0
35	31811.0
36	40058.0
37	65874.0
38	66715.0
39	41543.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.042524665681185	17.65443982261336	22.577549360010085	17.72548615169537
2	31.401331545716022	25.301087467198368	25.73538680142551	17.5621941856601
3	32.15190163521148	23.285720833763047	28.232321496098184	16.330056034927292
4	28.647713339521243	26.107520597705893	27.218765398145923	18.02600066462695
5	23.052528447179345	36.709179873262514	23.74179243012823	16.49649924942991
6	27.330491480170053	33.934648836329885	25.258975328589273	13.475884354910791
7	49.19270743809229	42.98532091168482	5.33391774668546	2.48805390353742
8	84.98229571315618	4.474199869366427	7.86379731169858	2.679707105778817
9	79.40630478875175	6.596422473558161	9.886039396335384	4.111233341354693
10	44.16360136133934	26.557289697136376	16.770371388955734	12.508737552568554
11	34.06499593202471	24.934683213585892	24.420456759141484	16.579864095247917
12	31.772032956329426	22.288493932414312	28.03808999965623	17.901383111600033
13	25.286763610528606	24.96189854125844	29.585925951390564	20.1654118968224
14	21.266916474726987	26.084029472767483	30.72180778530258	21.927246267202953
15	20.668465743064388	25.525972016913613	32.89960695337298	20.905955286649018
16	24.284666597912157	24.287817846379504	32.02384635658382	19.403669199124526
17	24.274639898243322	24.324773396587485	28.81100530555651	22.589581399612683
18	25.07477053181615	23.796509562606712	29.8110969782392	21.31762292733794
19	25.262986008456807	26.37251194609646	26.783320155385198	21.581181890061536
20	27.32877261736968	25.21056069304548	28.40907788740303	19.05158880218181
21	26.556143788602792	25.42971570009282	28.598725749710656	19.41541476159373
22	26.186301809389573	23.799660811074062	28.27099590910653	21.74304147042983
23	25.23634363505105	25.7359597556923	28.654588790722723	20.373107818533924
24	23.516334926146197	23.855523852086126	31.075034090778875	21.553107130988806
25	23.70569631132043	25.871463439788233	28.650291633721796	21.772548615169537
26	22.815038903594715	27.21303585547802	27.937250048701113	22.034675192226157
27	22.910722266148717	26.87040920393734	29.222099991978638	20.9967685379353
28	21.15662277836983	28.44345514341045	29.20290602404116	21.197016054178555
29	22.582705948411196	26.112677186107003	29.543240858514675	21.761376006967122
30	22.995805974767094	25.596158914595435	29.915947609061845	21.492087501575625
31	25.003151248467347	26.11582843457435	28.001420926581638	20.87959939037666
32	25.58412687499284	26.189166580723526	28.024912051520047	20.20179449276359
33	24.751910802479745	26.410040450571238	27.17550735100324	21.662541395945777
34	22.16903296778851	26.625184777751038	29.580769362989447	21.625012891471002
35	23.268818682892732	26.508875061592583	28.505907158490608	21.716399097024073
36	24.37719871199881	28.619352103315116	26.3223784477523	20.68107073693378
37	23.70025324578592	27.62212520196638	27.860474176951193	20.817147375296503
38	23.227852452817217	27.683144831379558	27.735856623924278	21.353146091878948
39	23.033620956375263	26.72029518603825	28.04811669932506	22.197967158261427
40	25.443180125362392	25.340048357340116	28.502469432889864	20.714302084407624
41	21.65824423894485	25.67494012627912	29.021279521468596	23.645536113307436
42	22.934213391087123	25.15269231209965	28.370976428661464	23.542117868151763
43	22.642293192157403	26.24216485040164	28.100828491869777	23.01471346557118
44	22.583565379811382	26.512312787193327	27.85130690868255	23.05281492431274
45	22.29365052081543	26.64609760848889	27.106752838988392	23.95349903170729
46	22.608775367550162	28.0518409020592	26.69623110683305	22.64315262355759
47	22.32401709695532	26.673026459028044	29.256763725119463	21.746192718897177
48	22.731101103509918	26.19976623465915	29.52834404757812	21.540788614252808
49	23.342729783308698	26.704252466568118	28.126038479608557	21.826979270514627
50	21.222512519050728	27.338799317038514	29.14389173456175	22.29479642934901
51	21.175530269173915	26.74779699084419	28.227451384830466	23.849221355151432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	225.0
1	665.5
2	1106.0
3	2709.5
4	4313.0
5	2941.0
6	1569.0
7	1438.5
8	1308.0
9	1292.5
10	1277.0
11	1272.0
12	1267.0
13	1210.0
14	1153.0
15	1150.5
16	1148.0
17	1172.0
18	1196.0
19	1195.0
20	1194.0
21	1373.5
22	1553.0
23	1751.5
24	1950.0
25	2231.5
26	3092.5
27	3672.0
28	4569.5
29	5467.0
30	6498.0
31	7529.0
32	8428.5
33	9328.0
34	10200.0
35	11072.0
36	11765.5
37	12459.0
38	13495.0
39	14531.0
40	15902.0
41	17273.0
42	18003.0
43	18733.0
44	19365.5
45	19998.0
46	20381.5
47	20765.0
48	21185.0
49	21605.0
50	21972.5
51	22340.0
52	22684.5
53	23029.0
54	22475.5
55	21922.0
56	21233.0
57	20544.0
58	19641.5
59	18739.0
60	17547.5
61	16356.0
62	14933.0
63	13510.0
64	11581.5
65	9653.0
66	8135.0
67	6617.0
68	5612.5
69	4608.0
70	3765.0
71	2922.0
72	2448.0
73	1974.0
74	1571.5
75	941.5
76	714.0
77	553.0
78	392.0
79	279.0
80	166.0
81	128.5
82	91.0
83	66.5
84	42.0
85	40.5
86	39.0
87	30.0
88	21.0
89	16.0
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	349068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.20369279862492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.72378455490728	26.423388438085016
2	9.663827994386352	7.770431438901208
3	5.602216495229235	6.75689372896756
4	4.16048451839493	6.690673658839389
5	3.1539206019256505	6.339962749553653
6	2.5418874344342934	6.131595692560871
7	2.018614022999618	5.680901664177111
8	1.6023617112121218	5.153668639188087
9	1.2865199316113485	4.6550566898829535
>10	4.215293151315941	22.62080241489584
>50	0.021690407151091634	0.5833910576065424
>100	0.0079531492887336	0.5130469440336558
>500	7.230135717030544E-4	0.25608745478393813
>1k	7.230135717030544E-4	0.42409942852413823
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1459	0.4179701376236149	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	881	0.25238635452118213	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.864771333952124E-4	0.0	0.0	0.011172608202413283	0.0
2	2.864771333952124E-4	0.0	0.0	0.05987372087959939	0.0
3	2.864771333952124E-4	0.0	0.0	0.07792178028349778	0.0
4	2.864771333952124E-4	0.0	0.0	0.10972074209036635	0.0
5	2.864771333952124E-4	0.0	0.0	0.21428489577961887	0.0
6	2.864771333952124E-4	0.0	0.0	0.3056711013326916	0.0
7	2.864771333952124E-4	0.0	0.0	0.3904683328176745	0.0
8	2.864771333952124E-4	0.0	0.0	0.6004560715963652	0.0
9	2.864771333952124E-4	0.0	0.0	0.6929881856830188	0.0
10	2.864771333952124E-4	0.0	0.0	0.9244617094663504	0.0
11	2.864771333952124E-4	0.0	0.0	1.1946096462580358	0.0
12	2.864771333952124E-4	0.0	0.0	1.4275155557083434	0.0
13	2.864771333952124E-4	0.0	0.0	1.5183288069946257	0.0
14	2.864771333952124E-4	0.0	0.0	1.5627327626708836	0.0
15	2.864771333952124E-4	0.0	0.0	1.6458111313554953	0.0
16	2.864771333952124E-4	0.0	0.0	1.80050878338891	0.0
17	2.864771333952124E-4	0.0	0.0	1.9483309842208394	0.0
18	2.864771333952124E-4	0.0	0.0	2.133395212394147	0.0
19	2.864771333952124E-4	0.0	0.0	2.251423791352974	0.0
20	2.864771333952124E-4	0.0	0.0	2.379192592847239	0.0
21	2.864771333952124E-4	0.0	0.0	2.5198528653442884	0.0
22	2.864771333952124E-4	0.0	0.0	2.6874419883804874	0.0
23	2.864771333952124E-4	0.0	0.0	2.856749974217058	0.0
24	2.864771333952124E-4	0.0	0.0	3.0002750180480593	0.0
25	2.864771333952124E-4	0.0	0.0	3.133486885076833	0.0
26	2.864771333952124E-4	0.0	0.0	3.2715688633733255	0.0
27	2.864771333952124E-4	0.0	0.0	3.4371526464757585	0.0
28	2.864771333952124E-4	0.0	0.0	3.5818235988403404	0.0
29	2.864771333952124E-4	0.0	0.0	3.750558630410121	0.0
30	2.864771333952124E-4	0.0	0.0	4.016122933067482	0.0
31	2.864771333952124E-4	0.0	0.0	4.2003277298406045	0.0
32	2.864771333952124E-4	0.0	0.0	4.389402637881444	0.0
33	2.864771333952124E-4	0.0	0.0	4.575612774588333	0.0
34	2.864771333952124E-4	0.0	0.0	4.739191217756999	0.0
35	2.864771333952124E-4	0.0	0.0	4.957200316270756	0.0
36	2.864771333952124E-4	0.0	0.0	5.152291244112895	0.0
37	2.864771333952124E-4	0.0	0.0	5.349387511888801	0.0
38	2.864771333952124E-4	0.0	0.0	5.585731146939851	0.0
39	2.864771333952124E-4	0.0	0.0	5.930363138414291	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	75	0.0	45.000004	10
CGCGGTA	20	7.026531E-4	45.0	31
TAGACGG	25	3.8852064E-5	45.0	2
TCGATAG	20	7.026531E-4	45.0	2
CCCCGAC	20	7.026531E-4	45.0	42
TTAACGT	20	7.026531E-4	45.0	17
TGTAACG	20	7.026531E-4	45.0	1
TACGTAG	20	7.026531E-4	45.0	2
CGTTTTA	230	0.0	44.021736	1
CGTTATT	110	0.0	42.954544	1
TAACGGG	90	0.0	42.5	3
AGGACCG	80	0.0	42.1875	7
TACGAAT	80	0.0	42.1875	12
CGAATAT	80	0.0	42.1875	14
CGCATGG	50	1.0768417E-9	40.5	2
CTCGATC	50	1.0768417E-9	40.5	16
CTACGAA	85	0.0	39.705883	11
GGCCTAT	80	0.0	39.375	8
TGACTAT	80	0.0	39.375	23
TTAACGG	35	6.235796E-6	38.571426	2
>>END_MODULE