FastQCFastQC Report
Sat 18 Jun 2016
SRR3549813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337935
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.6101765132347937No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.32639412904848564No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.1284270643762854No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA207.0262095E-445.00000434
TACATCG207.0262095E-445.00000419
CGGGCTA207.0262095E-445.0000046
GTGTACG207.0262095E-445.0000041
TTAACGG207.0262095E-445.0000042
TGTACGG406.7902874E-945.0000042
CCTGCGC207.0262095E-445.00000442
CGAACGG253.8849415E-545.02
TATGCGT253.8849415E-545.011
GCGTACG253.8849415E-545.01
GCCCGCT253.8849415E-545.023
GCGCGAA302.1609376E-644.9999961
CGTTTTA3900.043.8461531
CGTTTTT9950.042.512561
AGGCGAT1700.042.352947
CGAAGGA800.042.1875044
TTTGCGG603.6379788E-1241.2499962
GCGAGAC1150.041.08695621
TAGGCGA556.002665E-1140.9090926
CGGTTTT1300.039.807691
Produced by FastQC (version 0.11.5)