##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549813_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 337935 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9558879666208 33.0 31.0 34.0 30.0 34.0 2 32.130344592895085 33.0 31.0 34.0 30.0 34.0 3 31.867723082841376 33.0 31.0 34.0 30.0 34.0 4 35.71108941068549 37.0 35.0 37.0 35.0 37.0 5 32.02225280009469 37.0 35.0 37.0 0.0 37.0 6 33.94044416825721 37.0 35.0 37.0 19.0 37.0 7 21.016535724325685 32.0 0.0 37.0 0.0 37.0 8 28.274153313507036 35.0 17.0 37.0 17.0 37.0 9 34.988142690162306 37.0 32.0 39.0 32.0 39.0 10 36.29340257741873 37.0 35.0 39.0 32.0 39.0 11 36.66864633731339 38.0 35.0 39.0 33.0 39.0 12 37.093660023377275 39.0 37.0 39.0 34.0 39.0 13 36.93814490952402 39.0 37.0 39.0 33.0 39.0 14 38.372988888395696 40.0 38.0 41.0 34.0 41.0 15 38.53600840398301 40.0 38.0 41.0 34.0 41.0 16 38.58762187994733 40.0 38.0 41.0 34.0 41.0 17 38.542944649118915 40.0 38.0 41.0 34.0 41.0 18 38.43468714397739 40.0 38.0 41.0 34.0 41.0 19 38.354870019382425 40.0 37.0 41.0 34.0 41.0 20 38.3181233077367 40.0 37.0 41.0 34.0 41.0 21 38.21855682305769 40.0 37.0 41.0 34.0 41.0 22 38.18347019397221 40.0 37.0 41.0 34.0 41.0 23 38.09236095698877 40.0 37.0 41.0 34.0 41.0 24 37.970269430511784 40.0 36.0 41.0 33.0 41.0 25 37.826667850326245 40.0 36.0 41.0 33.0 41.0 26 37.71211031707281 40.0 36.0 41.0 33.0 41.0 27 37.66463373133887 40.0 36.0 41.0 33.0 41.0 28 37.586831787178006 40.0 36.0 41.0 33.0 41.0 29 37.59909745957063 40.0 36.0 41.0 33.0 41.0 30 37.439989938893575 40.0 36.0 41.0 33.0 41.0 31 37.316599346028084 40.0 35.0 41.0 33.0 41.0 32 37.179525648423514 40.0 35.0 41.0 32.0 41.0 33 36.97705771820024 40.0 35.0 41.0 31.0 41.0 34 36.76061372749197 40.0 35.0 41.0 31.0 41.0 35 36.56763874709633 40.0 35.0 41.0 30.0 41.0 36 36.36805598709811 39.0 35.0 41.0 30.0 41.0 37 36.262097740689775 39.0 35.0 41.0 29.0 41.0 38 36.15450012576383 39.0 35.0 41.0 29.0 41.0 39 36.09566632636454 39.0 35.0 41.0 29.0 41.0 40 36.01698847411485 39.0 35.0 41.0 28.0 41.0 41 35.92459496648764 39.0 35.0 41.0 27.0 41.0 42 35.81704173879592 39.0 35.0 40.0 27.0 41.0 43 35.71875952476068 39.0 35.0 40.0 26.0 41.0 44 35.58079808247148 39.0 35.0 40.0 26.0 41.0 45 35.48390370929321 39.0 35.0 40.0 26.0 41.0 46 35.35442614704011 38.0 35.0 40.0 25.0 41.0 47 35.3083048515247 38.0 35.0 40.0 25.0 41.0 48 35.19524464763934 38.0 34.0 40.0 24.0 41.0 49 35.06809889475787 38.0 34.0 40.0 24.0 41.0 50 34.93382455205883 38.0 34.0 40.0 24.0 41.0 51 33.202716498734965 36.0 31.0 40.0 19.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 8.0 10 20.0 11 9.0 12 8.0 13 6.0 14 13.0 15 7.0 16 29.0 17 45.0 18 92.0 19 222.0 20 341.0 21 684.0 22 1071.0 23 1833.0 24 2779.0 25 3869.0 26 4713.0 27 4840.0 28 5093.0 29 5490.0 30 6260.0 31 7852.0 32 10637.0 33 15414.0 34 21609.0 35 28435.0 36 37003.0 37 64271.0 38 69111.0 39 46159.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.338926124846495 18.13869531122849 22.86386435261219 19.658514211312827 2 31.27761255862814 24.781096956515306 26.733839347803574 17.207451137052985 3 30.61653868347463 23.80398597363398 30.269134596889934 15.31034074600145 4 26.58795330462959 25.916522408155412 29.251483273410567 18.24404101380443 5 21.691153624217673 37.174604583721724 25.333274150354356 15.800967641706245 6 24.920176957107135 35.22896414991049 26.77941024161451 13.071448651367866 7 49.6494888070191 42.05660851938982 6.305058665127909 1.988844008463166 8 85.61764836433042 3.824404101380443 8.577685057777384 1.9802624765117551 9 79.65614689215381 6.96021424238389 10.106677319602882 3.276961545859411 10 37.720271649873496 33.71565537751343 17.316643733262314 11.247429239350764 11 27.804459437465784 25.67328036456715 30.009025404293727 16.51323479367334 12 26.002337727669524 24.963380531759068 31.31756106943643 17.71672067113498 13 22.059863583233465 27.155813987897083 32.698299968928936 18.08602245994052 14 19.633361445248347 29.005577995768416 31.445692218917838 19.9153683400654 15 19.209907230680457 27.990589906342933 34.35956618876411 18.439936674212497 16 22.32559515883232 26.323997218399985 33.63901341974048 17.711394203027208 17 22.04270051933064 26.23107994140885 31.38355009099383 20.34266944826668 18 22.588959415272168 26.031633302262268 31.884238093124416 19.495169189341148 19 22.73099856481276 27.92193765073165 29.587938508884843 19.759125275570746 20 24.808617041738795 26.784736709722285 30.902984301714824 17.503661946824092 21 23.7365173776022 27.404382499593115 30.849719620637106 18.009380502167577 22 23.909923505999675 25.48478257653099 30.42182668264607 20.183467234823265 23 21.494666134019855 28.37675884415642 30.98228949354166 19.146285528282068 24 20.291180256558214 26.576412623729418 33.97014218710699 19.16226493260538 25 20.451270214686257 28.583603355674907 31.610516815363905 19.354609614274935 26 21.683163922056018 29.310370337490937 29.614570849423703 19.39189489102934 27 21.332208856732805 28.453104887034492 30.930504386938317 19.284181869294393 28 19.356681018539067 28.88040599523577 32.39972183999882 19.363191146226345 29 20.172518383712845 27.3049550949147 31.761729326645654 20.760797194726795 30 20.38824034207762 28.21666888602838 31.8857176675988 19.509373104295204 31 22.794916182106025 27.35792386109755 29.731457232899817 20.115702723896607 32 21.847692603607204 28.01958956604081 30.37566395904538 19.757053871306614 33 21.68050068800213 28.41670735496471 29.13015816651131 20.772633790521848 34 20.38912808676225 28.730081228638642 30.811250684303193 20.069540000295916 35 20.914377025167564 28.054211608741326 30.46266293813899 20.568748427952123 36 21.824019412017105 30.01168863834761 28.33444301418912 19.829848935446165 37 20.948703152973206 29.606877062156922 29.969668723275184 19.474751061594688 38 21.177149451817655 29.60776480684155 29.438205572077468 19.77688016926332 39 21.34197404826372 28.772397058605947 29.28403391184695 20.601594981283384 40 22.65317294746031 28.615266249426664 29.732640892479324 18.998919910633703 41 19.599035317442702 28.404278929379913 30.41087783153565 21.585807921641738 42 21.793540177844854 28.377054759051294 29.404767188956455 20.424637874147393 43 21.656531581517154 28.116945566455087 28.87715093139213 21.349371920635626 44 21.13631319632474 29.20709603917913 28.834243271635078 20.822347492861056 45 20.86111234408984 29.49413348720908 28.142690162309318 21.50206400639176 46 21.098732004675455 29.9670054892213 27.648512287866012 21.285750218237233 47 20.65545149215086 29.230473315874356 29.896577744240755 20.217497447734033 48 21.59823634722654 28.523828546909908 29.38405314631512 20.493881959548435 49 21.573675410951807 28.182934588012486 29.335523103555417 20.907866897480286 50 20.328169618417743 29.360675869619897 29.45862369982393 20.85253081213843 51 20.42286238477814 28.544838504446123 28.812641484309115 22.21965762646663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 180.0 1 501.5 2 823.0 3 3399.5 4 5976.0 5 4246.0 6 2516.0 7 2208.5 8 1901.0 9 1828.0 10 1755.0 11 1732.5 12 1710.0 13 1671.5 14 1633.0 15 1665.0 16 1697.0 17 1668.5 18 1640.0 19 1707.0 20 1774.0 21 1867.0 22 1960.0 23 2293.5 24 2627.0 25 3095.0 26 4388.0 27 5213.0 28 6045.0 29 6877.0 30 7862.0 31 8847.0 32 9988.0 33 11129.0 34 12633.0 35 14137.0 36 14773.0 37 15409.0 38 16523.0 39 17637.0 40 18880.5 41 20124.0 42 21458.5 43 22793.0 44 23150.0 45 23507.0 46 23545.5 47 23584.0 48 23481.5 49 23379.0 50 22841.5 51 22304.0 52 21383.0 53 20462.0 54 18911.5 55 17361.0 56 15590.0 57 13819.0 58 12420.5 59 11022.0 60 10227.0 61 9432.0 62 8264.5 63 7097.0 64 5891.5 65 4686.0 66 3898.5 67 3111.0 68 2619.5 69 2128.0 70 1764.5 71 1401.0 72 1238.0 73 1075.0 74 916.0 75 592.0 76 427.0 77 326.0 78 225.0 79 172.5 80 120.0 81 95.0 82 70.0 83 45.0 84 20.0 85 14.5 86 9.0 87 6.5 88 4.0 89 3.5 90 3.0 91 5.0 92 7.0 93 5.0 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 337935.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.62541439870141 #Duplication Level Percentage of deduplicated Percentage of total 1 69.3098724667568 31.622916532169242 2 10.195738864464822 9.303696215843058 3 5.422025235507596 7.421464447507521 4 3.711040399638606 6.772710243353354 5 2.7391930773320796 6.248840963566514 6 1.9873051094581036 5.440297149340958 7 1.5978541413162592 5.103193014236487 8 1.157097615841319 4.223444657800767 9 0.8745730555362676 3.5912482272702646 >10 2.980934008776481 18.307766924162035 >50 0.01843915433527834 0.573281807895776 >100 0.004609788583819585 0.4401770694797231 >500 0.0 0.0 >1k 0.0013170824525198814 0.9509627473742824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.6101765132347937 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1103 0.32639412904848564 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.1284270643762854 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013020255374554278 0.0 2 0.0 0.0 0.0 0.05622383002648439 0.0 3 0.0 0.0 0.0 0.08167251098584047 0.0 4 0.0 0.0 0.0 0.11155991536834006 0.0 5 0.0 0.0 0.0 0.20270170299022 0.0 6 0.0 0.0 0.0 0.3178125970970749 0.0 7 0.0 0.0 0.0 0.4184236613549943 0.0 8 0.0 0.0 0.0 0.677645109266575 0.0 9 0.0 0.0 0.0 0.7989702161658307 0.0 10 0.0 0.0 0.0 1.0333348129078077 0.0 11 0.0 0.0 0.0 1.2964031544527794 0.0 12 0.0 0.0 0.0 1.5127169426073062 0.0 13 0.0 0.0 0.0 1.6118484323908444 0.0 14 0.0 0.0 0.0 1.6728069007353485 0.0 15 0.0 0.0 0.0 1.7509284329826742 0.0 16 0.0 0.0 0.0 1.902732774054182 0.0 17 0.0 0.0 0.0 2.09774068977762 0.0 18 0.0 0.0 0.0 2.2820956692855137 0.0 19 0.0 0.0 0.0 2.405196265554027 0.0 20 0.0 0.0 0.0 2.535102904404693 0.0 21 0.0 0.0 0.0 2.7079172030124137 0.0 22 0.0 0.0 0.0 2.899670054892213 0.0 23 0.0 0.0 0.0 3.121902140944264 0.0 24 0.0 0.0 0.0 3.2840635033364403 0.0 25 0.0 0.0 0.0 3.4488880997825024 0.0 26 0.0 0.0 0.0 3.593886398271857 0.0 27 0.0 0.0 0.0 3.7628538032461862 0.0 28 0.0 0.0 0.0 3.94484146359507 0.0 29 0.0 0.0 0.0 4.124461804784944 0.0 30 0.0 0.0 0.0 4.340479678044594 0.0 31 0.0 0.0 0.0 4.545548700193824 0.0 32 2.959148948762336E-4 0.0 0.0 4.7420361903916435 0.0 33 2.959148948762336E-4 0.0 0.0 4.945329723171616 0.0 34 2.959148948762336E-4 0.0 0.0 5.150990575110598 0.0 35 2.959148948762336E-4 0.0 0.0 5.379436873955051 0.0 36 2.959148948762336E-4 0.0 0.0 5.572077470519479 0.0 37 2.959148948762336E-4 0.0 0.0 5.793717726781778 0.0 38 2.959148948762336E-4 0.0 0.0 6.010623344726056 0.0 39 5.918297897524672E-4 0.0 0.0 6.261263260686227 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCGTA 20 7.0262095E-4 45.000004 34 TACATCG 20 7.0262095E-4 45.000004 19 CGGGCTA 20 7.0262095E-4 45.000004 6 GTGTACG 20 7.0262095E-4 45.000004 1 TTAACGG 20 7.0262095E-4 45.000004 2 TGTACGG 40 6.7902874E-9 45.000004 2 CCTGCGC 20 7.0262095E-4 45.000004 42 CGAACGG 25 3.8849415E-5 45.0 2 TATGCGT 25 3.8849415E-5 45.0 11 GCGTACG 25 3.8849415E-5 45.0 1 GCCCGCT 25 3.8849415E-5 45.0 23 GCGCGAA 30 2.1609376E-6 44.999996 1 CGTTTTA 390 0.0 43.846153 1 CGTTTTT 995 0.0 42.51256 1 AGGCGAT 170 0.0 42.35294 7 CGAAGGA 80 0.0 42.187504 4 TTTGCGG 60 3.6379788E-12 41.249996 2 GCGAGAC 115 0.0 41.086956 21 TAGGCGA 55 6.002665E-11 40.909092 6 CGGTTTT 130 0.0 39.80769 1 >>END_MODULE