FastQCFastQC Report
Sat 18 Jun 2016
SRR3549812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469917
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.5030675629951673No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.28792318643505127No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.10661457236065093No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAG207.029026E-445.02
CGACAAT253.8872735E-545.020
CGGATTA207.029026E-445.019
GAGCGAT253.8872735E-545.06
ACAGCCG253.8872735E-545.023
TTACGAG207.029026E-445.02
CGCACGG351.2098826E-745.02
CGTTTAG253.8872735E-545.02
ATCGCCA253.8872735E-545.024
ATAACGC302.162753E-644.99999611
CGTTTTA5550.043.7837831
CGTTATT2000.041.6251
AGGCGAT3150.041.4285747
CGTTTTT10000.040.951
AAGGTAT900.040.0000046
TAGGCGA900.040.0000046
GGACCGA1800.040.0000048
ACGAAAG451.924127E-840.0000042
TATGGGC850.039.7058834
GACCGAT1700.039.7058839
Produced by FastQC (version 0.11.5)