##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549812_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469917
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.967794312612654	33.0	31.0	34.0	30.0	34.0
2	32.136566670284324	33.0	31.0	34.0	30.0	34.0
3	31.936586673816866	33.0	31.0	34.0	30.0	34.0
4	35.75744227172032	37.0	35.0	37.0	35.0	37.0
5	32.165175977885454	37.0	35.0	37.0	10.0	37.0
6	34.01786485698538	37.0	35.0	37.0	19.0	37.0
7	20.71086808947112	32.0	0.0	37.0	0.0	37.0
8	28.12226414451914	35.0	17.0	37.0	17.0	37.0
9	34.972029103011806	37.0	32.0	39.0	32.0	39.0
10	36.29216436094033	37.0	35.0	39.0	32.0	39.0
11	36.65375161996693	38.0	35.0	39.0	33.0	39.0
12	37.02263804033478	39.0	37.0	39.0	34.0	39.0
13	36.83109357609961	39.0	37.0	39.0	33.0	39.0
14	38.22355862843864	40.0	38.0	41.0	34.0	41.0
15	38.38142480480595	40.0	38.0	41.0	34.0	41.0
16	38.456103950272066	40.0	38.0	41.0	34.0	41.0
17	38.432127375685496	40.0	38.0	41.0	34.0	41.0
18	38.34450551905975	40.0	37.0	41.0	34.0	41.0
19	38.25992675302234	40.0	37.0	41.0	34.0	41.0
20	38.229102160594316	40.0	37.0	41.0	34.0	41.0
21	38.142129354758396	40.0	37.0	41.0	34.0	41.0
22	38.10781691234835	40.0	37.0	41.0	34.0	41.0
23	38.02172511315828	40.0	36.0	41.0	34.0	41.0
24	37.89336627532096	40.0	36.0	41.0	33.0	41.0
25	37.75623354762649	40.0	36.0	41.0	33.0	41.0
26	37.618113411517356	40.0	36.0	41.0	33.0	41.0
27	37.60041241325596	40.0	36.0	41.0	33.0	41.0
28	37.52606524130857	40.0	36.0	41.0	33.0	41.0
29	37.50079269317773	40.0	36.0	41.0	33.0	41.0
30	37.351044971771614	39.0	35.0	41.0	33.0	41.0
31	37.22604630179372	39.0	35.0	41.0	32.0	41.0
32	37.100102784108685	39.0	35.0	41.0	32.0	41.0
33	36.9183494106406	39.0	35.0	41.0	31.0	41.0
34	36.735517123236654	39.0	35.0	41.0	31.0	41.0
35	36.58443937972025	39.0	35.0	41.0	30.0	41.0
36	36.402368928981076	39.0	35.0	41.0	30.0	41.0
37	36.324921209490185	39.0	35.0	41.0	30.0	41.0
38	36.1809830246618	39.0	35.0	41.0	30.0	41.0
39	36.10661244432527	39.0	35.0	41.0	29.0	41.0
40	36.002713245104985	39.0	35.0	40.0	29.0	41.0
41	35.89987806357293	39.0	35.0	40.0	28.0	41.0
42	35.78467686846826	39.0	35.0	40.0	27.0	41.0
43	35.697274199486294	39.0	35.0	40.0	27.0	41.0
44	35.573614063760196	38.0	35.0	40.0	27.0	41.0
45	35.47497536799052	38.0	35.0	40.0	26.0	41.0
46	35.343307435142805	38.0	34.0	40.0	26.0	41.0
47	35.270175371395375	38.0	34.0	40.0	26.0	41.0
48	35.172217646946166	38.0	34.0	40.0	25.0	41.0
49	35.05087281370965	38.0	34.0	40.0	24.0	41.0
50	34.891934107512604	38.0	34.0	40.0	24.0	41.0
51	33.049761979243144	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	15.0
11	5.0
12	3.0
13	10.0
14	10.0
15	18.0
16	38.0
17	68.0
18	129.0
19	261.0
20	427.0
21	841.0
22	1418.0
23	2366.0
24	3513.0
25	4843.0
26	5868.0
27	6342.0
28	6804.0
29	7695.0
30	9274.0
31	11801.0
32	16006.0
33	23597.0
34	33115.0
35	42067.0
36	53539.0
37	88387.0
38	92225.0
39	59208.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.560105295190425	18.196830504110302	23.101526439775537	17.141537760923736
2	32.14823043218271	24.436443031428954	26.370827188631186	17.04449934775716
3	31.473004807231913	23.299859336861616	29.34156457416948	15.885571281736988
4	26.78983735425618	26.147809081178163	28.412889935882298	18.649463628683364
5	21.981967028219877	37.530670309863225	24.10404390562589	16.383318756291004
6	27.279498294379646	35.70162390379578	24.586469525469393	12.432408276355186
7	50.72917132174405	42.39450796630043	5.21368667232724	1.6626340396282746
8	87.82082793344357	2.968183743086545	7.48727966853721	1.7237086549326794
9	82.38518717135153	5.793150705337326	8.969456308241668	2.8522058150694694
10	40.31137413628364	33.23203884941383	15.232690028239029	11.223896986063496
11	28.580153516472055	25.46130486873214	29.094286863424816	16.864254751370986
12	27.869815307809674	23.18664785483394	30.437928400121724	18.50560843723466
13	23.094716726570862	26.288046612486887	32.00799290087398	18.609243760068267
14	20.16270958488414	27.033710208398503	31.5349306366869	21.26864957003045
15	19.214244217595873	26.165684578340432	34.87807421310572	19.74199699095798
16	22.710606341119817	24.62222051979392	33.66637086974934	19.000802269336926
17	22.650808547041287	24.625625376396258	31.002708989034232	21.720857087528223
18	22.94490303606807	24.557315440811887	32.078856478910104	20.418925044209935
19	22.529936137658353	26.889216606336863	29.95337474490176	20.627472511103022
20	24.288118965689687	26.313583037004406	30.737981388202595	18.660316609103308
21	23.74121387393944	26.798775102837308	30.929504572084003	18.530506451139246
22	23.98912999529704	24.804805955094196	30.301946939565923	20.90411711004284
23	21.817257090081867	26.59873977745006	30.68946218161931	20.894540950848768
24	21.108408506183007	25.23083863746151	34.086657856600205	19.574094999755275
25	20.874750221847687	26.401258094514564	31.492157125620057	21.23183455801769
26	21.00307075504823	28.246051962367822	29.92932794514776	20.821549337436185
27	20.85602351053484	27.81214554910761	30.769050704698913	20.562780235658636
28	19.421089256187795	27.636795434087297	32.21334831470239	20.728766995022525
29	20.30720318694578	25.315960052519916	32.12865250671928	22.248184253815033
30	21.207787758263695	27.06371550720659	31.2310471849284	20.497449549601313
31	22.88042356416133	26.784091658739733	29.37305949774109	20.962425279357845
32	22.69422046872107	27.198845753611806	30.068288655230607	20.038645122436517
33	22.88042356416133	26.725145078811792	29.79206966336608	20.602361693660797
34	20.844532119501956	26.78707090826678	30.615406550518497	21.75299042171277
35	22.122204559528598	25.887550354637096	30.890135917619492	21.10010916821481
36	22.973418710112636	27.75192214795379	28.928087300523288	20.34657184141029
37	22.274359088945495	28.260096995852457	29.688434340532478	19.77710957466957
38	22.044744071825452	27.378877546460334	29.627572528765718	20.9488058529485
39	22.107308311893377	27.991113324267904	29.237078037185288	20.66450032665343
40	23.97487215827476	26.60299584820298	29.241546911475858	20.180585082046402
41	20.423606722038148	25.71241304315443	30.90332973695355	22.960650497853877
42	20.903053092354607	26.041194508817515	30.08041845687643	22.97533394195145
43	21.02179746636108	26.66066560690505	30.037857749347225	22.279679177386644
44	20.737917547141304	27.940253278770506	29.16664006622446	22.15518910786373
45	21.343130808206556	27.71085106518811	28.50460826060772	22.441409865997613
46	21.76107695614332	28.241157481001967	27.658714198464835	22.33905136438988
47	20.759197900905903	27.18692875550363	30.40302861994778	21.650844723642688
48	21.868968349729844	26.33273535539255	30.308969456308244	21.489326838569365
49	22.002396167833894	26.605975097730024	29.789941627989624	21.60168710644646
50	21.393139639553368	27.122874890672183	29.73674074357812	21.747244726196328
51	21.28439703181626	26.47190886901304	28.810619747742688	23.43307435142802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	776.5
2	1279.0
3	4104.5
4	6930.0
5	4834.0
6	2738.0
7	2430.5
8	2123.0
9	2140.0
10	2157.0
11	2114.0
12	2071.0
13	2094.0
14	2117.0
15	2110.0
16	2103.0
17	2120.5
18	2138.0
19	2236.5
20	2335.0
21	2475.0
22	2615.0
23	2909.5
24	3204.0
25	3956.5
26	5565.0
27	6421.0
28	7418.0
29	8415.0
30	9878.5
31	11342.0
32	12750.0
33	14158.0
34	15618.5
35	17079.0
36	18265.5
37	19452.0
38	20830.5
39	22209.0
40	24107.5
41	26006.0
42	27547.0
43	29088.0
44	30395.5
45	31703.0
46	31905.5
47	32108.0
48	32361.0
49	32614.0
50	32157.0
51	31700.0
52	30467.5
53	29235.0
54	27039.0
55	24843.0
56	22792.0
57	20741.0
58	19644.0
59	18547.0
60	17508.0
61	16469.0
62	14614.5
63	12760.0
64	11051.0
65	9342.0
66	7887.0
67	6432.0
68	5292.0
69	4152.0
70	3572.5
71	2993.0
72	2565.0
73	2137.0
74	1743.5
75	1094.0
76	838.0
77	656.5
78	475.0
79	363.5
80	252.0
81	192.5
82	133.0
83	97.0
84	61.0
85	48.0
86	35.0
87	25.5
88	16.0
89	13.5
90	11.0
91	7.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.61406063236667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04582332134535	32.142567299672784
2	8.83078937718041	7.879547454103726
3	4.461687939633566	5.97162048784533
4	2.962426240228725	5.286634556019135
5	2.2363196838938046	4.988565098529662
6	1.826517930473327	4.889302901774514
7	1.4762451269670926	4.6102902721921994
8	1.2122541126851762	4.326686278813785
9	1.0017062643674268	4.022116561287891
>10	3.921306985024516	24.05004826188678
>50	0.01881942190661054	0.5738853484023175
>100	0.004577697220520608	0.30157913326448266
>500	5.086330245022897E-4	0.11368784481979369
>1k	0.0010172660490045794	0.843468501387571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2364	0.5030675629951673	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1353	0.28792318643505127	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	501	0.10661457236065093	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022769978528123052	0.0
2	0.0	0.0	0.0	0.09618719901599644	0.0
3	0.0	0.0	0.0	0.1278949261252519	0.0
4	0.0	0.0	0.0	0.16300750983684353	0.0
5	0.0	0.0	0.0	0.2857951510585912	0.0
6	0.0	0.0	0.0	0.4222022186896835	0.0
7	0.0	0.0	0.0	0.5486075200514133	0.0
8	0.0	0.0	0.0	0.8529165788852073	0.0
9	0.0	0.0	0.0	0.9899620571292377	0.0
10	0.0	0.0	0.0	1.3515152675898083	0.0
11	0.0	0.0	0.0	1.9209775343305306	0.0
12	0.0	0.0	0.0	2.3272194876967633	0.0
13	0.0	0.0	0.0	2.5387462041168973	0.0
14	0.0	0.0	0.0	2.6330181712940797	0.0
15	0.0	0.0	0.0	2.7851727007109766	0.0
16	0.0	0.0	0.0	3.0524539439943648	0.0
17	0.0	0.0	0.0	3.4146455650678735	0.0
18	0.0	0.0	0.0	3.781944471044887	0.0
19	0.0	0.0	0.0	4.016879576606081	0.0
20	0.0	0.0	0.0	4.253942717543737	0.0
21	0.0	0.0	0.0	4.553782902086964	0.0
22	0.0	0.0	0.0	4.884692403126509	0.0
23	0.0	0.0	0.0	5.240074310995346	0.0
24	0.0	0.0	0.0	5.524379837290415	0.0
25	0.0	0.0	0.0	5.779744082465627	0.0
26	0.0	0.0	0.0	6.01531759863976	0.0
27	0.0	0.0	0.0	6.267915397825574	0.0
28	0.0	0.0	0.0	6.531153373893687	0.0
29	0.0	0.0	0.0	6.80354190208058	0.0
30	0.0	0.0	0.0	7.128705707603683	0.0
31	0.0	0.0	0.0	7.433865980588061	0.0
32	0.0	0.0	0.0	7.746687180927696	0.0
33	0.0	0.0	0.0	8.029928689534534	0.0
34	0.0	0.0	0.0	8.335727373131851	0.0
35	0.0	0.0	0.0	8.645782127482088	0.0
36	0.0	0.0	0.0	8.940940634197103	0.0
37	0.0	0.0	0.0	9.251420995622631	0.0
38	0.0	0.0	0.0	9.566370231338725	0.0
39	0.0	0.0	0.0	9.893023661625351	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAG	20	7.029026E-4	45.0	2
CGACAAT	25	3.8872735E-5	45.0	20
CGGATTA	20	7.029026E-4	45.0	19
GAGCGAT	25	3.8872735E-5	45.0	6
ACAGCCG	25	3.8872735E-5	45.0	23
TTACGAG	20	7.029026E-4	45.0	2
CGCACGG	35	1.2098826E-7	45.0	2
CGTTTAG	25	3.8872735E-5	45.0	2
ATCGCCA	25	3.8872735E-5	45.0	24
ATAACGC	30	2.162753E-6	44.999996	11
CGTTTTA	555	0.0	43.783783	1
CGTTATT	200	0.0	41.625	1
AGGCGAT	315	0.0	41.428574	7
CGTTTTT	1000	0.0	40.95	1
AAGGTAT	90	0.0	40.000004	6
TAGGCGA	90	0.0	40.000004	6
GGACCGA	180	0.0	40.000004	8
ACGAAAG	45	1.924127E-8	40.000004	2
TATGGGC	85	0.0	39.705883	4
GACCGAT	170	0.0	39.705883	9
>>END_MODULE