FastQCFastQC Report
Sat 18 Jun 2016
SRR3549811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499152
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.35199698688976505No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.19793569894541144No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.029448E-445.021
CGCGATA207.029448E-445.017
CGCGAAG253.8876242E-545.02
TACGATA207.029448E-445.031
TACGAAT600.044.99999612
CGTTTTA3950.042.1518971
TAGGCGA650.041.538466
GCGCAAA556.002665E-1140.9090921
AGGCGAT3100.040.645167
AAGGCGA2050.040.6097566
CGTTATT1450.040.3448261
CGATAAG403.4529876E-739.3752
ACACGAC2850.038.6842126
ACGACCA2850.038.6842128
AGGACCG1050.038.571437
CTACGAA700.038.57142611
CGAATAT700.038.57142614
TGTAGCG356.241189E-638.5714261
CGCAATC700.038.57142620
GCGAGAC2950.038.13559321
Produced by FastQC (version 0.11.5)