##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549811_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499152
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.984309388723275	33.0	31.0	34.0	30.0	34.0
2	32.165949450267654	33.0	31.0	34.0	30.0	34.0
3	32.03242499278777	33.0	31.0	34.0	30.0	34.0
4	35.80859938455621	37.0	35.0	37.0	35.0	37.0
5	32.07966511203	37.0	35.0	37.0	0.0	37.0
6	33.96806383626631	37.0	35.0	37.0	19.0	37.0
7	20.855494919383275	32.0	0.0	37.0	0.0	37.0
8	28.168509792608262	35.0	17.0	37.0	17.0	37.0
9	34.95289410840786	37.0	32.0	39.0	32.0	39.0
10	36.24760794307145	37.0	35.0	39.0	32.0	39.0
11	36.611046334583456	38.0	35.0	39.0	32.0	39.0
12	36.981065887745615	39.0	37.0	39.0	33.0	39.0
13	36.779986857710675	39.0	37.0	39.0	33.0	39.0
14	38.170693496169505	40.0	37.0	41.0	33.0	41.0
15	38.346447575087346	40.0	38.0	41.0	34.0	41.0
16	38.41183647466102	40.0	38.0	41.0	34.0	41.0
17	38.375911545981985	40.0	38.0	41.0	34.0	41.0
18	38.27334960092317	40.0	37.0	41.0	34.0	41.0
19	38.19195154982851	40.0	37.0	41.0	34.0	41.0
20	38.1675862262397	40.0	37.0	41.0	34.0	41.0
21	38.08974620957143	40.0	37.0	41.0	34.0	41.0
22	38.054446340994325	40.0	37.0	41.0	34.0	41.0
23	37.967018062634224	40.0	36.0	41.0	33.0	41.0
24	37.83674311632529	40.0	36.0	41.0	33.0	41.0
25	37.69098991890246	40.0	36.0	41.0	33.0	41.0
26	37.55359289354745	39.0	36.0	41.0	33.0	41.0
27	37.52937381799532	40.0	36.0	41.0	33.0	41.0
28	37.455099849344485	40.0	36.0	41.0	33.0	41.0
29	37.44502876879187	40.0	36.0	41.0	33.0	41.0
30	37.26860154822579	39.0	35.0	41.0	32.0	41.0
31	37.15661161329615	39.0	35.0	41.0	32.0	41.0
32	37.0346788152707	39.0	35.0	41.0	31.0	41.0
33	36.907278744751096	39.0	35.0	41.0	31.0	41.0
34	36.738843077860054	39.0	35.0	41.0	31.0	41.0
35	36.57646167900759	39.0	35.0	41.0	30.0	41.0
36	36.397586306375615	39.0	35.0	41.0	30.0	41.0
37	36.31155038946053	39.0	35.0	41.0	30.0	41.0
38	36.175008814950154	39.0	35.0	40.0	30.0	41.0
39	36.090824037567714	39.0	35.0	40.0	29.0	41.0
40	35.993757412571725	39.0	35.0	40.0	29.0	41.0
41	35.88990928614931	39.0	35.0	40.0	28.0	41.0
42	35.792053322434846	39.0	35.0	40.0	28.0	41.0
43	35.67688199185819	38.0	35.0	40.0	27.0	41.0
44	35.5168225310126	38.0	35.0	40.0	27.0	41.0
45	35.41112126165977	38.0	34.0	40.0	26.0	41.0
46	35.25681355579062	38.0	34.0	40.0	26.0	41.0
47	35.19917580216046	38.0	34.0	40.0	26.0	41.0
48	35.11100426323044	38.0	34.0	40.0	26.0	41.0
49	34.990984710068275	38.0	34.0	40.0	26.0	41.0
50	34.8381515049524	38.0	34.0	40.0	24.0	41.0
51	32.982009488091805	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	11.0
11	9.0
12	11.0
13	5.0
14	14.0
15	23.0
16	49.0
17	103.0
18	192.0
19	314.0
20	571.0
21	883.0
22	1433.0
23	2354.0
24	3499.0
25	4700.0
26	5715.0
27	6420.0
28	7358.0
29	8732.0
30	10385.0
31	13178.0
32	17627.0
33	25856.0
34	35900.0
35	45747.0
36	57949.0
37	94724.0
38	95385.0
39	59981.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13575824598519	17.555774593710936	22.181820367343015	17.126646792960862
2	31.490808411065167	24.166786870532423	26.07562425874283	18.266780459659582
3	32.25891111324807	23.72303426611533	27.429520466711544	16.588534153925057
4	27.27986665384492	26.321240824438245	27.445547648812386	18.953344872904445
5	22.233708369394492	38.16552873673751	23.12281629643876	16.47794659742924
6	27.28207039138379	36.28493925697984	23.91335705356284	12.519633298073533
7	51.907835689329104	41.99121710420874	4.431916530435618	1.6690306760265412
8	88.62430682437414	3.301399172997404	6.467568997018944	1.6067250056095137
9	83.28084431195308	5.712488380292976	8.007180177581178	2.999487130172773
10	41.999030355482894	30.15975093759015	14.952359201205246	12.888859505721703
11	30.692454402666925	26.596106997467707	25.774713914799502	16.936724685065872
12	28.44904958810142	23.42412731993461	29.115179344167707	19.01164374779626
13	23.891520017950445	26.163172740968683	30.531581562329713	19.413725678751163
14	21.38246466006347	26.457471872295415	30.746345802481006	21.41371766516011
15	21.006026220469916	25.717016059236464	32.9923550341379	20.28460268615572
16	24.459082604096547	24.368929704779305	31.502027438535755	19.66996025258839
17	24.75398275475206	24.244719043497774	28.98596018847966	22.015338013270508
18	24.779225566560886	23.952222970157386	29.33475173894926	21.933799724332466
19	24.440851684456838	26.339872423630478	27.98746674359714	21.23180914831554
20	26.11428983556111	26.107077603615732	28.373922172003716	19.40471038881944
21	25.89732185787095	25.98667339808315	28.32263518928102	19.79336955476488
22	25.41129756066288	24.38535756643267	28.069806391640224	22.13353848126422
23	23.440755521364235	26.522181620027567	28.95370548450171	21.083357374106484
24	22.645406609609896	25.08875052088342	31.77529089335513	20.490551976151554
25	23.010626021732858	25.830809052152446	29.603808058467163	21.55475686764753
26	22.743573099977564	27.404277654902714	28.21785748629676	21.63429175882296
27	22.298618456902908	27.165272301823894	28.956510241369358	21.579598999903837
28	21.008430297785043	27.602213353848125	29.63345834535372	21.75589800301311
29	21.723442959258904	25.497644004231173	29.77910536269513	22.99980767381479
30	22.797063820239124	26.261940250665127	29.65609674007116	21.284899189024586
31	23.91676282975927	26.264745007532774	27.948200147450073	21.870292015257878
32	24.119707023111197	26.475302112382597	28.54741641824534	20.85757444626086
33	23.709611501105876	26.279570150976056	27.62765650543321	22.383161842484856
34	22.108896688784178	26.89902073917364	28.559036125268456	22.433046446773727
35	22.770218290220214	26.98516684296567	28.65299548033465	21.59161938647947
36	23.941604962015578	28.33826169182934	26.66141776452864	21.058715581626437
37	22.863576625957624	28.469484245279997	27.64869218194057	21.018246946821808
38	23.03707087219925	27.965429368208483	27.77410488187967	21.2233948777126
39	23.065919799980765	27.54812161425778	26.889604769689395	22.49635381607206
40	23.986881751450458	26.625156265025485	27.782318812706354	21.605643170817707
41	20.792263679199923	26.233492002436133	29.064092701221274	23.910151617142674
42	22.53041157803635	26.008911113248068	27.62805718498574	23.832620123729846
43	22.435250184312594	26.33025611436997	28.038152706991053	23.196340994326377
44	21.70901849536814	28.307008686732697	27.63807417379876	22.345898644100394
45	22.417620284001664	26.989574318043402	27.25642690002244	23.336378497932493
46	22.241922300221177	27.496033272430044	26.98937397826714	23.27267044908164
47	21.62928326441645	26.958721992499278	28.916241946340993	22.495752796743275
48	22.752187710356765	26.425016828541203	28.57846908356573	22.244326377536304
49	22.312241561688623	27.295493156393242	28.292784562618202	22.099480719299933
50	21.853463474051992	27.188712055646373	28.58287655864346	22.37494791165817
51	21.898740263486875	27.087540468634803	27.457568035388018	23.5561512324903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	1006.5
2	1774.0
3	3600.5
4	5427.0
5	3841.0
6	2255.0
7	2027.5
8	1800.0
9	1794.5
10	1789.0
11	1809.5
12	1830.0
13	1853.5
14	1877.0
15	1843.5
16	1810.0
17	1842.0
18	1874.0
19	1954.5
20	2035.0
21	1994.5
22	1954.0
23	2268.5
24	2583.0
25	3284.5
26	4713.5
27	5441.0
28	6444.0
29	7447.0
30	8724.0
31	10001.0
32	11677.5
33	13354.0
34	14647.5
35	15941.0
36	17233.5
37	18526.0
38	19969.5
39	21413.0
40	23913.5
41	26414.0
42	27917.0
43	29420.0
44	30361.5
45	31303.0
46	31695.5
47	32088.0
48	32818.5
49	33549.0
50	33780.0
51	34011.0
52	32789.5
53	31568.0
54	30095.5
55	28623.0
56	27422.0
57	26221.0
58	24830.5
59	23440.0
60	21807.0
61	20174.0
62	18311.5
63	16449.0
64	14524.5
65	12600.0
66	11019.5
67	9439.0
68	8070.0
69	6701.0
70	5696.0
71	4691.0
72	4034.5
73	3378.0
74	2909.0
75	1947.0
76	1454.0
77	1157.0
78	860.0
79	660.0
80	460.0
81	362.0
82	264.0
83	185.0
84	106.0
85	92.0
86	78.0
87	48.5
88	19.0
89	18.5
90	18.0
91	16.0
92	14.0
93	13.0
94	12.0
95	7.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	499152.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.48083537137016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93270851705212	28.309344605528032
2	8.766337768072512	7.097373519983359
3	4.587243057713996	5.570862930833428
4	3.1548958713982627	5.108512815355539
5	2.4171793803581307	4.8924720279674005
6	1.9486609938464783	4.733005493190591
7	1.5747961237690997	4.462434384083815
8	1.2660346700625758	4.100011284260005
9	1.114073039627375	4.0588746577953945
>10	5.212904952342295	30.150056144558548
>50	0.0193985031878065	0.5405612467158675
>100	0.0047185548294604775	0.3939072139394149
>500	5.24283869940053E-4	0.20968767638585234
>1k	5.24283869940053E-4	0.3728959994027759
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1757	0.35199698688976505	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	988	0.19793569894541144	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0033977626053787E-4	0.0	0.0	0.013823444561977113	0.0
2	2.0033977626053787E-4	0.0	0.0	0.052489021380260924	0.0
3	2.0033977626053787E-4	0.0	0.0	0.07372503766387793	0.0
4	2.0033977626053787E-4	0.0	0.0	0.0973651312626214	0.0
5	2.0033977626053787E-4	0.0	0.0	0.17690002243805494	0.0
6	2.0033977626053787E-4	0.0	0.0	0.2766692310158028	0.0
7	2.0033977626053787E-4	0.0	0.0	0.35780684040132066	0.0
8	2.0033977626053787E-4	0.0	0.0	0.5815863704843415	0.0
9	2.0033977626053787E-4	0.0	0.0	0.6737426675641889	0.0
10	2.0033977626053787E-4	0.0	0.0	0.8987242363047729	0.0
11	2.0033977626053787E-4	0.0	0.0	1.182605699265955	0.0
12	2.0033977626053787E-4	0.0	0.0	1.4007757156136809	0.0
13	2.0033977626053787E-4	0.0	0.0	1.5157707471872295	0.0
14	2.0033977626053787E-4	0.0	0.0	1.5804804949193834	0.0
15	2.0033977626053787E-4	0.0	0.0	1.663020482738725	0.0
16	2.0033977626053787E-4	0.0	0.0	1.8236929832996762	0.0
17	2.0033977626053787E-4	0.0	0.0	2.0240327595602143	0.0
18	2.0033977626053787E-4	0.0	0.0	2.2406000576978555	0.0
19	2.0033977626053787E-4	0.0	0.0	2.3928582876558644	0.0
20	2.0033977626053787E-4	0.0	0.0	2.5367022470109304	0.0
21	2.0033977626053787E-4	0.0	0.0	2.729429111773568	0.0
22	2.0033977626053787E-4	0.0	0.0	2.9451950508061673	0.0
23	2.0033977626053787E-4	0.0	0.0	3.1743837548482228	0.0
24	2.0033977626053787E-4	0.0	0.0	3.3669102798345993	0.0
25	2.0033977626053787E-4	0.0	0.0	3.552224572875597	0.0
26	2.0033977626053787E-4	0.0	0.0	3.724917460012181	0.0
27	2.0033977626053787E-4	0.0	0.0	3.890398115203385	0.0
28	2.0033977626053787E-4	0.0	0.0	4.083124979966023	0.0
29	2.0033977626053787E-4	0.0	0.0	4.293481745039587	0.0
30	2.0033977626053787E-4	0.0	0.0	4.545509183575343	0.0
31	2.0033977626053787E-4	0.0	0.0	4.758270025964035	0.0
32	2.0033977626053787E-4	0.0	0.0	4.976640382088021	0.0
33	2.0033977626053787E-4	0.0	0.0	5.208633842997724	0.0
34	2.0033977626053787E-4	0.0	0.0	5.416386190979902	0.0
35	2.0033977626053787E-4	0.0	0.0	5.658596980478892	0.0
36	2.0033977626053787E-4	0.0	0.0	5.870556463762541	0.0
37	2.0033977626053787E-4	0.0	0.0	6.102750264448504	0.0
38	2.0033977626053787E-4	0.0	0.0	6.366197070231112	0.0
39	2.0033977626053787E-4	0.0	0.0	6.65608872648011	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.029448E-4	45.0	21
CGCGATA	20	7.029448E-4	45.0	17
CGCGAAG	25	3.8876242E-5	45.0	2
TACGATA	20	7.029448E-4	45.0	31
TACGAAT	60	0.0	44.999996	12
CGTTTTA	395	0.0	42.151897	1
TAGGCGA	65	0.0	41.53846	6
GCGCAAA	55	6.002665E-11	40.909092	1
AGGCGAT	310	0.0	40.64516	7
AAGGCGA	205	0.0	40.609756	6
CGTTATT	145	0.0	40.344826	1
CGATAAG	40	3.4529876E-7	39.375	2
ACACGAC	285	0.0	38.68421	26
ACGACCA	285	0.0	38.68421	28
AGGACCG	105	0.0	38.57143	7
CTACGAA	70	0.0	38.571426	11
CGAATAT	70	0.0	38.571426	14
TGTAGCG	35	6.241189E-6	38.571426	1
CGCAATC	70	0.0	38.571426	20
GCGAGAC	295	0.0	38.135593	21
>>END_MODULE