SRR3549810_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371948
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	673	0.1809392710809038	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	604	0.16238829083635348	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	495	0.13308311914568702	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	409	0.10996160753653737	Illumina PCR Primer Index 9 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG	396	0.10646649531654963	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	373	0.10028283523503284	Illumina PCR Primer Index 9 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	20	7.027126E-4	45.0	12
ACAAACG	20	7.027126E-4	45.0	33
GGCACGT	20	7.027126E-4	45.0	34
ACTTCGA	20	7.027126E-4	45.0	10
GACTTCG	20	7.027126E-4	45.0	9
GTTGTCC	25	3.8857004E-5	45.0	9
CTACGAA	80	0.0	45.0	11
GTACTTG	20	7.027126E-4	45.0	38
CTCGCTA	20	7.027126E-4	45.0	14
GCGATAT	20	7.027126E-4	45.0	9
ACGTCCA	20	7.027126E-4	45.0	37
ACCACGC	20	7.027126E-4	45.0	25
TATAGCG	20	7.027126E-4	45.0	1
CGATCAC	25	3.8857004E-5	45.0	12
ACGACGA	25	3.8857004E-5	45.0	33
TGCAACG	25	3.8857004E-5	45.0	1
ACACGAC	30	2.161527E-6	44.999996	26
ACCTATG	65	0.0	44.999996	34
CGTTTAA	30	2.161527E-6	44.999996	15
TACGAAT	85	0.0	42.35294	12