SRR3549808_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	663497
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	5234	0.7888505901307769	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	4941	0.7446906316079802	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	3879	0.584629621535591	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2093	0.3154498061031173	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1702	0.25651962254539207	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	936	0.14107072074176671	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	720	0.10851593903212825	Illumina PCR Primer Index 5 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	698	0.10520017422836878	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	688	0.10369300840847812	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	25	3.8890168E-5	45.0	43
GGTCGAT	20	7.0311275E-4	45.0	1
CCGTGAC	25	3.8890168E-5	45.0	31
TGACCGA	20	7.0311275E-4	45.0	26
CTCGCGG	25	3.8890168E-5	45.0	2
GCGATAT	55	1.8189894E-12	45.0	9
TTTGCGC	20	7.0311275E-4	45.0	13
AATAGCG	25	3.8890168E-5	45.0	1
TAGTCCG	20	7.0311275E-4	45.0	1
ATTTCGG	20	7.0311275E-4	45.0	2
TACGGCT	610	0.0	42.786884	7
TACGGGT	90	0.0	42.500004	4
ACGGCTG	695	0.0	41.43885	8
TATACGG	60	3.6379788E-12	41.249996	2
GCGTATG	55	6.002665E-11	40.909092	1
CGTTTTT	1320	0.0	40.56818	1
TGCGGGT	95	0.0	40.263157	4
TCGATCA	45	1.9261279E-8	40.000004	17
TTGTCGC	40	3.4552795E-7	39.375	38
CAAACGG	35	6.244296E-6	38.571426	2